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. 1989 Mar;86(5):1703–1707. doi: 10.1073/pnas.86.5.1703

Cell cultures of neuroblasts from rat olfactory epithelium that show odorant responses.

H G Coon 1, F Curcio 1, K Sakaguchi 1, M L Brandi 1, R D Swerdlow 1
PMCID: PMC286769  PMID: 2537981

Abstract

We have developed procedures that permit isolation and propagation of clonal cell cultures from the olfactory epithelium of the 5- to 7-day-old rat that appear to represent the neuroblasts that repopulate the sensory neurons in the olfactory epithelium throughout life. The cell lines we report here synthesize neuron-specific enolase, which is a neuron marker, 43-kDa growth-associated protein, a protein associated with neuronal growth cones, and carnosine, a possible olfactory neurotransmitter. In two of the cell lines we have found dose-dependent cAMP accumulation following exposure to submicromolar concentrations of chemical odorants in the medium. These two cell lines show different patterns of odorant specificity when tested against a panel of six chemicals commonly used as test odorants. We anticipate that these and similarly derived cell lines will prove valuable in studying aspects of neurogenesis and olfaction.

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Selected References

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