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. 1989 Mar;86(6):2036–2039. doi: 10.1073/pnas.86.6.2036

Anti-idiotypic antibodies that protect cells against the action of diphtheria toxin.

J M Rolf 1, H M Gaudin 1, S M Tirrell 1, A B MacDonald 1, L Eidels 1
PMCID: PMC286841  PMID: 2467297

Abstract

An anti-idiotypic serum prepared against the combining site (idiotype) of specific anti-diphtheria toxoid antibodies was characterized with respect to its interaction with highly diphtheria toxin-sensitive Vero cells. Although the anti-idiotypic serum protected Vero cells against the cytotoxic action of diphtheria toxin, it did not prevent the binding of 125I-labeled diphtheria toxin to the cells but did inhibit the internalization and degradation of 125I-labeled toxin. This anti-idiotypic serum immunoprecipitated a cell-surface protein from radiolabeled Vero cells with an apparent Mr of approximately 15,000. These results are consistent with the hypothesis that the anti-idiotypic serum contains antibodies that carry an internal image of an internalization site on the toxin and that a cell-surface protein involved in toxin internalization possesses a complementary site recognized by both the toxin and the anti-idiotypic antibodies.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cieplak W., Gaudin H. M., Eidels L. Diphtheria toxin receptor. Identification of specific diphtheria toxin-binding proteins on the surface of Vero and BS-C-1 cells. J Biol Chem. 1987 Sep 25;262(27):13246–13253. [PubMed] [Google Scholar]
  2. Collier R. J. Diphtheria toxin: mode of action and structure. Bacteriol Rev. 1975 Mar;39(1):54–85. doi: 10.1128/br.39.1.54-85.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Didsbury J. R., Moehring J. M., Moehring T. J. Binding and uptake of diphtheria toxin by toxin-resistant Chinese hamster ovary and mouse cells. Mol Cell Biol. 1983 Jul;3(7):1283–1294. doi: 10.1128/mcb.3.7.1283. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dorland R. B., Middlebrook J. L., Leppla S. H. Receptor-mediated internalization and degradation of diphtheria toxin by monkey kidney cells. J Biol Chem. 1979 Nov 25;254(22):11337–11342. [PubMed] [Google Scholar]
  5. Eidels L., Hart D. A. Effect of polymers of L-lysine on the cytotoxic action of diphtheria toxin. Infect Immun. 1982 Sep;37(3):1054–1058. doi: 10.1128/iai.37.3.1054-1058.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Eidels L., Proia R. L., Hart D. A. Membrane receptors for bacterial toxins. Microbiol Rev. 1983 Dec;47(4):596–620. doi: 10.1128/mr.47.4.596-620.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Eidels L., Ross L. L., Hart D. A. Diphtheria toxin-receptor interaction: a polyphosphate-insensitive diphtheria toxin-binding domain. Biochem Biophys Res Commun. 1982 Nov 30;109(2):493–499. doi: 10.1016/0006-291x(82)91748-x. [DOI] [PubMed] [Google Scholar]
  8. Falmagne P., Capiau C., Lambotte P., Zanen J., Cabiaux V., Ruysschaert J. M. The complete amino acid sequence of diphtheria toxin fragment B. Correlation with its lipid-binding properties. Biochim Biophys Acta. 1985 Jan 21;827(1):45–50. doi: 10.1016/0167-4838(85)90099-8. [DOI] [PubMed] [Google Scholar]
  9. Hayakawa S., Uchida T., Mekada E., Moynihan M. R., Okada Y. Monoclonal antibody against diphtheria toxin. Effect on toxin binding and entry into cells. J Biol Chem. 1983 Apr 10;258(7):4311–4317. [PubMed] [Google Scholar]
  10. Islam M. N., Pepper B. M., Briones-Urbina R., Farid N. R. Biological activity of anti-thyrotropin anti-idiotypic antibody. Eur J Immunol. 1983 Jan;13(1):57–63. doi: 10.1002/eji.1830130113. [DOI] [PubMed] [Google Scholar]
  11. Keen J. H., Maxfield F. R., Hardegree M. C., Habig W. H. Receptor-mediated endocytosis of diphtheria toxin by cells in culture. Proc Natl Acad Sci U S A. 1982 May;79(9):2912–2916. doi: 10.1073/pnas.79.9.2912. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lambris J. D., Ross G. D. Characterization of the lymphocyte membrane receptor for factor H (beta 1H-globulin) with an antibody to anti-factor H idiotype. J Exp Med. 1982 May 1;155(5):1400–1411. doi: 10.1084/jem.155.5.1400. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Marnell M. H., Shia S. P., Stookey M., Draper R. K. Evidence for penetration of diphtheria toxin to the cytosol through a prelysosomal membrane. Infect Immun. 1984 Apr;44(1):145–150. doi: 10.1128/iai.44.1.145-150.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Mekada E., Okada Y., Uchida T. Identification of diphtheria toxin receptor and a nonproteinous diphtheria toxin-binding molecule in Vero cell membrane. J Cell Biol. 1988 Aug;107(2):511–519. doi: 10.1083/jcb.107.2.511. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Middlebrook J. L., Dorland R. B. Bacterial toxins: cellular mechanisms of action. Microbiol Rev. 1984 Sep;48(3):199–221. doi: 10.1128/mr.48.3.199-221.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Middlebrook J. L., Dorland R. B., Leppla S. H. Association of diphtheria toxin with Vero cells. Demonstration of a receptor. J Biol Chem. 1978 Oct 25;253(20):7325–7330. [PubMed] [Google Scholar]
  17. Morris R. E., Gerstein A. S., Bonventre P. F., Saelinger C. B. Receptor-mediated entry of diphtheria toxin into monkey kidney (Vero) cells: electron microscopic evaluation. Infect Immun. 1985 Dec;50(3):721–727. doi: 10.1128/iai.50.3.721-727.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Pain D., Kanwar Y. S., Blobel G. Identification of a receptor for protein import into chloroplasts and its localization to envelope contact zones. Nature. 1988 Jan 21;331(6153):232–237. doi: 10.1038/331232a0. [DOI] [PubMed] [Google Scholar]
  19. Pappenheimer A. M., Jr Diphtheria toxin. Annu Rev Biochem. 1977;46:69–94. doi: 10.1146/annurev.bi.46.070177.000441. [DOI] [PubMed] [Google Scholar]
  20. Pappenheimer A. M., Jr, Uchida T., Harper A. A. An immunological study of the diphtheria toxin molecule. Immunochemistry. 1972 Sep;9(9):891–906. doi: 10.1016/0019-2791(72)90163-2. [DOI] [PubMed] [Google Scholar]
  21. Proia R. L., Eidels L., Hart D. A. Diphtheria toxin:receptor interaction. Characterization of the receptor interaction with the nucleotide-free toxin, the nucleotide-bound toxin, and the B-fragment of the toxin. J Biol Chem. 1981 May 25;256(10):4991–4997. [PubMed] [Google Scholar]
  22. Schreiber A. B., Couraud P. O., Andre C., Vray B., Strosberg A. D. Anti-alprenolol anti-idiotypic antibodies bind to beta-adrenergic receptors and modulate catecholamine-sensitive adenylate cyclase. Proc Natl Acad Sci U S A. 1980 Dec;77(12):7385–7389. doi: 10.1073/pnas.77.12.7385. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Sege K., Peterson P. A. Use of anti-idiotypic antibodies as cell-surface receptor probes. Proc Natl Acad Sci U S A. 1978 May;75(5):2443–2447. doi: 10.1073/pnas.75.5.2443. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Shechter Y., Maron R., Elias D., Cohen I. R. Autoantibodies to insulin receptor spontaneously develop as anti-idiotypes in mice immunized with insulin. Science. 1982 Apr 30;216(4545):542–545. doi: 10.1126/science.7041258. [DOI] [PubMed] [Google Scholar]

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