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. 1989 Apr;86(8):2789–2793. doi: 10.1073/pnas.86.8.2789

Gene-controlled cytosine demethylation in the promoter region of the Ac transposable element in maize

Drew Schwartz 1
PMCID: PMC287004  PMID: 16594028

Abstract

The transposase product of the Ac transposable element causes demethylation of the BamHI site in the promoter region of Ac. This site remains methylated in Ds9, the deletional derivative of Ac that fails to make an active transposase. In the presence of an active Ac element the BamHI site of Ds9 is demethylated. Transcription of the element is correlated with the demethylation of the promoter region. Evidence is presented that supports the model that the trans-acting protein causes site-specific demethylation by inducing a configuration change in chromatin structure making the site inaccessible to the methylase. A chromosome alteration that involves the insertion of the 4.5-kilobase Ac element results in the methylation of Pvu I target sites about 1 kilobase removed from the point of insertion.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Busslinger M., Hurst J., Flavell R. A. DNA methylation and the regulation of globin gene expression. Cell. 1983 Aug;34(1):197–206. doi: 10.1016/0092-8674(83)90150-2. [DOI] [PubMed] [Google Scholar]
  2. Cedar H., Stein R., Gruenbaum Y., Naveh-Many T., Sciaky-Gallili N., Razin A. Effect of DNA methylation on gene expression. Cold Spring Harb Symp Quant Biol. 1983;47(Pt 2):605–609. doi: 10.1101/sqb.1983.047.01.071. [DOI] [PubMed] [Google Scholar]
  3. Chandler V. L., Walbot V. DNA modification of a maize transposable element correlates with loss of activity. Proc Natl Acad Sci U S A. 1986 Mar;83(6):1767–1771. doi: 10.1073/pnas.83.6.1767. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chomet P. S., Wessler S., Dellaporta S. L. Inactivation of the maize transposable element Activator (Ac) is associated with its DNA modification. EMBO J. 1987 Feb;6(2):295–302. doi: 10.1002/j.1460-2075.1987.tb04753.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Fedoroff N., Wessler S., Shure M. Isolation of the transposable maize controlling elements Ac and Ds. Cell. 1983 Nov;35(1):235–242. doi: 10.1016/0092-8674(83)90226-x. [DOI] [PubMed] [Google Scholar]
  6. Keshet I., Yisraeli J., Cedar H. Effect of regional DNA methylation on gene expression. Proc Natl Acad Sci U S A. 1985 May;82(9):2560–2564. doi: 10.1073/pnas.82.9.2560. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kessler C., Höltke H. J. Specificity of restriction endonucleases and methylases--a review. Gene. 1986;47(1):1–153. doi: 10.1016/0378-1119(86)90245-3. [DOI] [PubMed] [Google Scholar]
  8. Kunze R., Stochaj U., Laufs J., Starlinger P. Transcription of transposable element Activator (Ac) of Zea mays L. EMBO J. 1987 Jun;6(6):1555–1563. doi: 10.1002/j.1460-2075.1987.tb02400.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Osterman J. C., Schwartz D. Analysis of a controlling-element mutation at the adh locus of maize. Genetics. 1981 Oct;99(2):267–273. doi: 10.1093/genetics/99.2.267. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Pohlman R. F., Fedoroff N. V., Messing J. The nucleotide sequence of the maize controlling element Activator. Cell. 1984 Jun;37(2):635–643. doi: 10.1016/0092-8674(84)90395-7. [DOI] [PubMed] [Google Scholar]
  11. Razin A., Riggs A. D. DNA methylation and gene function. Science. 1980 Nov 7;210(4470):604–610. doi: 10.1126/science.6254144. [DOI] [PubMed] [Google Scholar]
  12. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  13. Sutton W. D., Gerlach W. L., Peacock W. J., Schwartz D. Molecular analysis of ds controlling element mutations at the adh1 locus of maize. Science. 1984 Mar 23;223(4642):1265–1268. doi: 10.1126/science.223.4642.1265. [DOI] [PubMed] [Google Scholar]
  14. Wessler S. R., Varagona M. J. Molecular basis of mutations at the waxy locus of maize: correlation with the fine structure genetic map. Proc Natl Acad Sci U S A. 1985 Jun;82(12):4177–4181. doi: 10.1073/pnas.82.12.4177. [DOI] [PMC free article] [PubMed] [Google Scholar]

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