Abstract
Two cDNAs encoding two human receptors for retinoic acid (RA), RAR-alpha and RAR-beta, have been characterized recently. Synthetic peptides corresponding to the cDNA-deduced amino acid sequences unique to RAR-alpha and RAR-beta were used to generate anti-RAR-alpha antiserum (SP171) and anti-RAR-beta antisera (SP172 and SP248). The specificity of these antisera was confirmed both by immunocytochemical detection of these receptors in COS-1 cells transfected with RAR-alpha and RAR-beta expression vectors and by immunoblot analyses performed with whole extracts of these cells. We also demonstrate that these antisera recognize RAR-alpha and RAR-beta endogenously expressed in the RA-responsive human promyelocytic leukemia cell line HL-60.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Benbrook D., Lernhardt E., Pfahl M. A new retinoic acid receptor identified from a hepatocellular carcinoma. Nature. 1988 Jun 16;333(6174):669–672. doi: 10.1038/333669a0. [DOI] [PubMed] [Google Scholar]
- Brand N., Petkovich M., Krust A., Chambon P., de Thé H., Marchio A., Tiollais P., Dejean A. Identification of a second human retinoic acid receptor. Nature. 1988 Apr 28;332(6167):850–853. doi: 10.1038/332850a0. [DOI] [PubMed] [Google Scholar]
- Hashimoto Y., Kagechika H., Kawachi E., Shudo K. Specific uptake of retinoids into human promyelocytic leukemia cells HL-60 by retinoid-specific binding protein: possibly the true retinoid receptor. Jpn J Cancer Res. 1988 Apr;79(4):473–483. doi: 10.1111/j.1349-7006.1988.tb01616.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jetten A. M., Anderson K., Deas M. A., Kagechika H., Lotan R., Rearick J. I., Shudo K. New benzoic acid derivatives with retinoid activity: lack of direct correlation between biological activity and binding to cellular retinoic acid binding protein. Cancer Res. 1987 Jul 1;47(13):3523–3527. [PubMed] [Google Scholar]
- Ladoux A., Cragoe E. J., Jr, Geny B., Abita J. P., Frelin C. Differentiation of human promyelocytic HL 60 cells by retinoic acid is accompanied by an increase in the intracellular pH. The role of the Na+/H+ exchange system. J Biol Chem. 1987 Jan 15;262(2):811–816. [PubMed] [Google Scholar]
- Lotan R. Effects of vitamin A and its analogs (retinoids) on normal and neoplastic cells. Biochim Biophys Acta. 1980 Mar 12;605(1):33–91. doi: 10.1016/0304-419x(80)90021-9. [DOI] [PubMed] [Google Scholar]
- Matzner Y., Gavison R., Rachmilewitz E. A., Fibach E. Expression of granulocytic functions by leukemic promyelocytic HL-60 cells: differential induction by dimethylsulfoxide and retinoic acid. Cell Differ. 1987 Sep;21(4):261–269. doi: 10.1016/0045-6039(87)90481-7. [DOI] [PubMed] [Google Scholar]
- Ong D. E., Crow J. A., Chytil F. Radioimmunochemical determination of cellular retinol- and cellular retinoic acid-binding proteins in cytosols of rat tissues. J Biol Chem. 1982 Nov 25;257(22):13385–13389. [PubMed] [Google Scholar]
- Petkovich M., Brand N. J., Krust A., Chambon P. A human retinoic acid receptor which belongs to the family of nuclear receptors. Nature. 1987 Dec 3;330(6147):444–450. doi: 10.1038/330444a0. [DOI] [PubMed] [Google Scholar]
- Picard D., Yamamoto K. R. Two signals mediate hormone-dependent nuclear localization of the glucocorticoid receptor. EMBO J. 1987 Nov;6(11):3333–3340. doi: 10.1002/j.1460-2075.1987.tb02654.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Van Regenmortel M. H., Daney de Marcillac G. An assessment of prediction methods for locating continuous epitopes in proteins. Immunol Lett. 1988 Feb;17(2):95–107. doi: 10.1016/0165-2478(88)90076-4. [DOI] [PubMed] [Google Scholar]
- Yen A., Brown D., Fishbaugh J. Precommitment states induced during HL-60 myeloid differentiation: possible similarities of retinoic acid- and DMSO-induced early events. Exp Cell Res. 1987 Nov;173(1):80–84. doi: 10.1016/0014-4827(87)90333-8. [DOI] [PubMed] [Google Scholar]