Skip to main content
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1989 May;86(9):3232–3236. doi: 10.1073/pnas.86.9.3232

The Tc2 transposon in Caenorhabditis elegans.

A Levitt 1, S W Emmons 1
PMCID: PMC287104  PMID: 2541437

Abstract

A second family of transposons, named Tc2 elements, has been identified in the nematode Caenorhabditis elegans. Tc2 elements are polymorphic in sequence and are present in different numbers in different strains. Like the transposon Tc1, Tc2 is active in the germ line of some C. elegans strains. A high rate of transposition has been observed in the progeny of certain interstrain crosses, where transposition events are frequent enough to be detected in blot hybridization experiments, without the use of a genetic screen. Our data suggest that transposition of Tc1 and Tc2 may be regulated by the same genomic factors.

Full text

PDF
3232

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bucheton A., Paro R., Sang H. M., Pelisson A., Finnegan D. J. The molecular basis of I-R hybrid dysgenesis in Drosophila melanogaster: identification, cloning, and properties of the I factor. Cell. 1984 Aug;38(1):153–163. doi: 10.1016/0092-8674(84)90536-1. [DOI] [PubMed] [Google Scholar]
  2. Collins J., Saari B., Anderson P. Activation of a transposable element in the germ line but not the soma of Caenorhabditis elegans. Nature. 1987 Aug 20;328(6132):726–728. doi: 10.1038/328726a0. [DOI] [PubMed] [Google Scholar]
  3. Döring H. P., Starlinger P. Barbara McClintock's controlling elements: now at the DNA level. Cell. 1984 Dec;39(2 Pt 1):253–259. doi: 10.1016/0092-8674(84)90002-3. [DOI] [PubMed] [Google Scholar]
  4. Eide D., Anderson P. The gene structures of spontaneous mutations affecting a Caenorhabditis elegans myosin heavy chain gene. Genetics. 1985 Jan;109(1):67–79. doi: 10.1093/genetics/109.1.67. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Eide D., Anderson P. Transposition of Tc1 in the nematode Caenorhabditis elegans. Proc Natl Acad Sci U S A. 1985 Mar;82(6):1756–1760. doi: 10.1073/pnas.82.6.1756. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Emmons S. W., Yesner L. High-frequency excision of transposable element Tc 1 in the nematode Caenorhabditis elegans is limited to somatic cells. Cell. 1984 Mar;36(3):599–605. doi: 10.1016/0092-8674(84)90339-8. [DOI] [PubMed] [Google Scholar]
  7. Emmons S. W., Yesner L., Ruan K. S., Katzenberg D. Evidence for a transposon in Caenorhabditis elegans. Cell. 1983 Jan;32(1):55–65. doi: 10.1016/0092-8674(83)90496-8. [DOI] [PubMed] [Google Scholar]
  8. Greenwald I. lin-12, a nematode homeotic gene, is homologous to a set of mammalian proteins that includes epidermal growth factor. Cell. 1985 Dec;43(3 Pt 2):583–590. doi: 10.1016/0092-8674(85)90230-2. [DOI] [PubMed] [Google Scholar]
  9. Krieg P. A., Melton D. A. Functional messenger RNAs are produced by SP6 in vitro transcription of cloned cDNAs. Nucleic Acids Res. 1984 Sep 25;12(18):7057–7070. doi: 10.1093/nar/12.18.7057. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Liao L. W., Rosenzweig B., Hirsh D. Analysis of a transposable element in Caenorhabditis elegans. Proc Natl Acad Sci U S A. 1983 Jun;80(12):3585–3589. doi: 10.1073/pnas.80.12.3585. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. McCLINTOCK B. Chromosome organization and genic expression. Cold Spring Harb Symp Quant Biol. 1951;16:13–47. doi: 10.1101/sqb.1951.016.01.004. [DOI] [PubMed] [Google Scholar]
  12. McClintock B. The significance of responses of the genome to challenge. Science. 1984 Nov 16;226(4676):792–801. doi: 10.1126/science.15739260. [DOI] [PubMed] [Google Scholar]
  13. Moerman D. G., Benian G. M., Waterston R. H. Molecular cloning of the muscle gene unc-22 in Caenorhabditis elegans by Tc1 transposon tagging. Proc Natl Acad Sci U S A. 1986 Apr;83(8):2579–2583. doi: 10.1073/pnas.83.8.2579. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Moerman D. G., Waterston R. H. Spontaneous unstable unc-22 IV mutations in C. elegans var. Bergerac. Genetics. 1984 Dec;108(4):859–877. doi: 10.1093/genetics/108.4.859. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Mori I., Moerman D. G., Waterston R. H. Analysis of a mutator activity necessary for germline transposition and excision of Tc1 transposable elements in Caenorhabditis elegans. Genetics. 1988 Oct;120(2):397–407. doi: 10.1093/genetics/120.2.397. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Rose A. M., Harris L. J., Mawji N. R., Morris W. J. Tc1(Hin): a form of the transposable element Tc1 in Caenorhabditis elegans. Can J Biochem Cell Biol. 1985 Jul;63(7):752–756. doi: 10.1139/o85-094. [DOI] [PubMed] [Google Scholar]
  17. Rose A. M., Snutch T. P. Isolation of the closed circular form of the transposable element Tc1 in Caenorhabditis elegans. Nature. 1984 Oct 4;311(5985):485–486. doi: 10.1038/311485a0. [DOI] [PubMed] [Google Scholar]
  18. Rosenzweig B., Liao L. W., Hirsh D. Sequence of the C. elegans transposable element Tc1. Nucleic Acids Res. 1983 Jun 25;11(12):4201–4209. doi: 10.1093/nar/11.12.4201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Ruan K., Emmons S. W. Extrachromosomal copies of transposon Tc1 in the nematode Caenorhabditis elegans. Proc Natl Acad Sci U S A. 1984 Jul;81(13):4018–4022. doi: 10.1073/pnas.81.13.4018. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Rubin G. M., Kidwell M. G., Bingham P. M. The molecular basis of P-M hybrid dysgenesis: the nature of induced mutations. Cell. 1982 Jul;29(3):987–994. doi: 10.1016/0092-8674(82)90462-7. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES