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. 1989 May;86(9):3394–3398. doi: 10.1073/pnas.86.9.3394

Atriopeptin inhibition of thrombin-mediated changes in the morphology and permeability of endothelial monolayers.

D A Baron 1, C E Lofton 1, W H Newman 1, M G Currie 1
PMCID: PMC287139  PMID: 2524068

Abstract

To determine the role of endothelial atriopeptin (AP) receptors, we examined the effect of AP-III on the morphology and macromolecular permeability of monolayer cultures of bovine aortic endothelial cells. AP-III alone (10(-9)-10(-6) M) had no observable effect on the morphology of the monolayers or their permeability to 125I-labeled albumin. However, incubation of the endothelial monolayers with AP-III (10(-8)-10(-6) M) antagonized thrombin-induced (1 unit/ml) cell-shape change and the formation of intercellular gaps. AP-III also opposed the effect of thrombin on the distribution of actin filaments in the endothelial cytoskeleton. Further, thrombin caused a 2-fold increase in monolayer permeability to 125I-labeled albumin, which was abolished by 10(-8)-10(-6) M AP-III pretreatment. Taken together with the findings that AP-III exhibited specific and saturable binding in these cells, these data suggest that AP regulates endothelial permeability through a receptor-mediated process.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Almeida F. A., Suzuki M., Maack T. Atrial natriuretic factor increases hematocrit and decreases plasma volume in nephrectomized rats. Life Sci. 1986 Sep 29;39(13):1193–1199. doi: 10.1016/0024-3205(86)90351-6. [DOI] [PubMed] [Google Scholar]
  2. Bianchi C., Gutkowska J., Thibault G., Garcia R., Genest J., Cantin M. Radioautographic localization of 125I-atrial natriuretic factor (ANF) in rat tissues. Histochemistry. 1985;82(5):441–452. doi: 10.1007/BF02450479. [DOI] [PubMed] [Google Scholar]
  3. Budzik G. P., Firestone S. L., Bush E. N., Connolly P. J., Rockway T. W., Sarin V. K., Holleman W. H. Divergence of ANF analogs in smooth muscle cell cGMP response and aorta vasorelaxation: evidence for receptor subtypes. Biochem Biophys Res Commun. 1987 Apr 14;144(1):422–431. doi: 10.1016/s0006-291x(87)80527-2. [DOI] [PubMed] [Google Scholar]
  4. Currie M. G., Geller D. M., Cole B. R., Siegel N. R., Fok K. F., Adams S. P., Eubanks S. R., Galluppi G. R., Needleman P. Purification and sequence analysis of bioactive atrial peptides (atriopeptins). Science. 1984 Jan 6;223(4631):67–69. doi: 10.1126/science.6419347. [DOI] [PubMed] [Google Scholar]
  5. Currie M. G., Oehlenschlager W. F., Kurtz D. T. Profound elevation of ventricular and pulmonary atriopeptin in a model of heart failure. Biochem Biophys Res Commun. 1987 Nov 13;148(3):1158–1164. doi: 10.1016/s0006-291x(87)80254-1. [DOI] [PubMed] [Google Scholar]
  6. Eskin S. G., Sybers H. D., Trevino L., Lie J. T., Chimoskey J. E. Comparison of tissue-cultured bovine endothelial cells from aorta and saphenous vein. In Vitro. 1978 Nov;14(11):903–910. doi: 10.1007/BF02616119. [DOI] [PubMed] [Google Scholar]
  7. Fraker P. J., Speck J. C., Jr Protein and cell membrane iodinations with a sparingly soluble chloroamide, 1,3,4,6-tetrachloro-3a,6a-diphrenylglycoluril. Biochem Biophys Res Commun. 1978 Feb 28;80(4):849–857. doi: 10.1016/0006-291x(78)91322-0. [DOI] [PubMed] [Google Scholar]
  8. Garcia J. G., Siflinger-Birnboim A., Bizios R., Del Vecchio P. J., Fenton J. W., 2nd, Malik A. B. Thrombin-induced increase in albumin permeability across the endothelium. J Cell Physiol. 1986 Jul;128(1):96–104. doi: 10.1002/jcp.1041280115. [DOI] [PubMed] [Google Scholar]
  9. Haynes L. W., Kay A. R., Yau K. W. Single cyclic GMP-activated channel activity in excised patches of rod outer segment membrane. Nature. 1986 May 1;321(6065):66–70. doi: 10.1038/321066a0. [DOI] [PubMed] [Google Scholar]
  10. Hoyer L. W., De los Santos R. P., Hoyer J. R. Antihemophilic factor antigen. Localization in endothelial cells by immunofluorescent microscopy. J Clin Invest. 1973 Nov;52(11):2737–2744. doi: 10.1172/JCI107469. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Imamura T., Ohnuma N., Iwasa F., Furuya M., Hayashi Y., Inomata N., Ishihara T., Noguchi T. Protective effect of alpha-human atrial natriuretic polypeptide (alpha-hANP) on chemical-induced pulmonary edema. Life Sci. 1988;42(4):403–414. doi: 10.1016/0024-3205(88)90078-1. [DOI] [PubMed] [Google Scholar]
  12. Inomata N., Ohnuma N., Furuya M., Hayashi Y., Kanai Y., Ishihara T., Noguchi T., Matsuo H. Alpha-human atrial natriuretic peptide (alpha-hANP) prevents pulmonary edema induced by arachidonic acid treatment in isolated perfused lung from guinea pig. Jpn J Pharmacol. 1987 Jun;44(2):211–214. doi: 10.1254/jjp.44.211. [DOI] [PubMed] [Google Scholar]
  13. Joyce N. C., DeCamilli P., Boyles J. Pericytes, like vascular smooth muscle cells, are immunocytochemically positive for cyclic GMP-dependent protein kinase. Microvasc Res. 1984 Sep;28(2):206–219. doi: 10.1016/0026-2862(84)90018-9. [DOI] [PubMed] [Google Scholar]
  14. Leitman D. C., Andresen J. W., Kuno T., Kamisaki Y., Chang J. K., Murad F. Identification of multiple binding sites for atrial natriuretic factor by affinity cross-linking in cultured endothelial cells. J Biol Chem. 1986 Sep 5;261(25):11650–11655. [PubMed] [Google Scholar]
  15. MAJNO G., PALADE G. E. Studies on inflammation. 1. The effect of histamine and serotonin on vascular permeability: an electron microscopic study. J Biophys Biochem Cytol. 1961 Dec;11:571–605. doi: 10.1083/jcb.11.3.571. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Mackie K., Lai Y., Nairn A. C., Greengard P., Pitt B. R., Lazo J. S. Protein phosphorylation in cultured endothelial cells. J Cell Physiol. 1986 Sep;128(3):367–374. doi: 10.1002/jcp.1041280304. [DOI] [PubMed] [Google Scholar]
  17. Miller F. N., Sims D. E. Contractile elements in the regulation of macromolecular permeability. Fed Proc. 1986 Feb;45(2):84–88. [PubMed] [Google Scholar]
  18. Murad F., Leitman D. C., Bennett B. M., Molina C., Waldman S. A. Regulation of guanylate cyclase by atrial natriuretic factor and the role of cyclic GMP in vasodilation. Am J Med Sci. 1987 Sep;294(3):139–143. doi: 10.1097/00000441-198709000-00003. [DOI] [PubMed] [Google Scholar]
  19. Needleman P., Greenwald J. E. Atriopeptin: a cardiac hormone intimately involved in fluid, electrolyte, and blood-pressure homeostasis. N Engl J Med. 1986 Mar 27;314(13):828–834. doi: 10.1056/NEJM198603273141306. [DOI] [PubMed] [Google Scholar]
  20. Rashatwar S. S., Cornwell T. L., Lincoln T. M. Effects of 8-bromo-cGMP on Ca2+ levels in vascular smooth muscle cells: possible regulation of Ca2+-ATPase by cGMP-dependent protein kinase. Proc Natl Acad Sci U S A. 1987 Aug;84(16):5685–5689. doi: 10.1073/pnas.84.16.5685. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Shasby D. M., Lind S. E., Shasby S. S., Goldsmith J. C., Hunninghake G. W. Reversible oxidant-induced increases in albumin transfer across cultured endothelium: alterations in cell shape and calcium homeostasis. Blood. 1985 Mar;65(3):605–614. [PubMed] [Google Scholar]
  22. Shepro D. The American Microcirculatory Society Landis Award lecture. Endothelial cells, inflammatory edema, and the microvascular barrier: comments by a "free radical". Microvasc Res. 1988 May;35(3):246–264. doi: 10.1016/0026-2862(88)90080-5. [DOI] [PubMed] [Google Scholar]
  23. Steiner A. L., Pagliara A. S., Chase L. R., Kipnis D. M. Radioimmunoassay for cyclic nucleotides. II. Adenosine 3',5'-monophosphate and guanosine 3',5'-monophosphate in mammalian tissues and body fluids. J Biol Chem. 1972 Feb 25;247(4):1114–1120. [PubMed] [Google Scholar]
  24. Stolz B., Bereiter-Hahn J. Increase of cytosolic calcium results in formation of F-actin aggregates in endothelial cells. Cell Biol Int Rep. 1988 Apr;12(4):321–329. doi: 10.1016/0309-1651(88)90077-x. [DOI] [PubMed] [Google Scholar]
  25. Trippodo N. C., Barbee R. W. Atrial natriuretic factor decreases whole-body capillary absorption in rats. Am J Physiol. 1987 May;252(5 Pt 2):R915–R920. doi: 10.1152/ajpregu.1987.252.5.R915. [DOI] [PubMed] [Google Scholar]
  26. Voyta J. C., Via D. P., Butterfield C. E., Zetter B. R. Identification and isolation of endothelial cells based on their increased uptake of acetylated-low density lipoprotein. J Cell Biol. 1984 Dec;99(6):2034–2040. doi: 10.1083/jcb.99.6.2034. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Welles S. L., Shepro D., Hechtman H. B. Vasoactive amines modulate actin cables (stress fibers) and surface area in cultured bovine endothelium. J Cell Physiol. 1985 Jun;123(3):337–342. doi: 10.1002/jcp.1041230307. [DOI] [PubMed] [Google Scholar]
  28. Zimmerman A. L., Baylor D. A. Cyclic GMP-sensitive conductance of retinal rods consists of aqueous pores. Nature. 1986 May 1;321(6065):70–72. doi: 10.1038/321070a0. [DOI] [PubMed] [Google Scholar]

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