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. Author manuscript; available in PMC: 2011 Jun 20.
Published in final edited form as: Virology. 2010 Apr 9;402(1):135–148. doi: 10.1016/j.virol.2010.03.009

Figure 6. Lentiviral over-expression of CCR5 increases the proportion of R5X4 entry mediated by CCR5 on CD4+ lymphocytes.

Figure 6

CD4+ lymphocytes were stimulated with PHA for 3 days, transduced with either control or CCR5-expressing lentiviruses, and maintained in the presence of IL-2. Two days post-transduction, cells were pretreated for 1 hour with CXCR4 blocker AMD3100 and infected with HIV-1 luciferase pseudotypes in the continued presence of blocker. Infection was determined by luciferase activity in cell lysates four days later. The results are shown as the proportion of CD4+ lymphocyte infection mediated through CCR5 for R3A (A), DH12 (B) and 89.6 (C) on the Y-axis, plotted against the percentage of CD4+ lymphocytes that express CCR5 after transduction with a control or CCR5 expression vectors. Data are from three independent experiments using cells from different donors.