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. 1989 Jun;86(12):4492–4495. doi: 10.1073/pnas.86.12.4492

Blue-light regulation of transcription for nuclear genes in pea

Kathleen A Marrs 1, Lon S Kaufman 1,*
PMCID: PMC287296  PMID: 16578843

Abstract

The expression of many nuclear genes in plants is light regulated. Previous investigations have shown that the excitation of phytochrome can affect transcription rate and steady-state RNA levels for several of these genes. No direct demonstration of the effects of blue light has been reported. We have identified several nuclear genes in pea whose transcription rate and steady-state RNA levels are affected by a single pulse of blue light. Pea seedlings, grown in continuous red light to saturate any phytochrome response, were treated with a single pulse (103 μmol·m-2) of blue light 6 days after planting. The blue-light treatment resulted in an increase in the steady-state RNA level and rate of transcription for the Cab (chlorophyll a/b binding protein) gene family and a decrease in the steady-state RNA level and the rate of transcription for the gene families corresponding to cDNA clones pEA25 and pEA207. Changes in the rate of transcription for Cab and pEA207 are apparent within 3 hr of the blue-light treatment.

Keywords: photoregulation, blue-light receptor, light-regulated gene expression, Cab, Pisum sativum

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Broglie R., Bellemare G., Bartlett S. G., Chua N. H., Cashmore A. R. Cloned DNA sequences complementary to mRNAs encoding precursors to the small subunit of ribulose-1,5-bisphosphate carboxylase and a chlorophyll a/b binding polypeptide. Proc Natl Acad Sci U S A. 1981 Dec;78(12):7304–7308. doi: 10.1073/pnas.78.12.7304. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cosgrove D. J. Rapid Suppression of Growth by Blue Light: OCCURRENCE, TIME COURSE, AND GENERAL CHARACTERISTICS. Plant Physiol. 1981 Mar;67(3):584–590. doi: 10.1104/pp.67.3.584. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Dobres M. S., Thompson W. F. A developmentally regulated bud specific transcript in pea has sequence similarity to seed lectins. Plant Physiol. 1989 Mar;89(3):833–838. doi: 10.1104/pp.89.3.833. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Fluhr R., Chua N. H. Developmental regulation of two genes encoding ribulose-bisphosphate carboxylase small subunit in pea and transgenic petunia plants: Phytochrome response and blue-light induction. Proc Natl Acad Sci U S A. 1986 Apr;83(8):2358–2362. doi: 10.1073/pnas.83.8.2358. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Fluhr Robert, Moses Phyllis, Morelli Giorgio, Coruzzi Gloria, Chua Nam-Hai. Expression dynamics of the pea rbcS multigene family and organ distribution of the transcripts. EMBO J. 1986 Sep;5(9):2063–2071. doi: 10.1002/j.1460-2075.1986.tb04467.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gallagher T. F., Ellis R. J. Light-stimulated transcription of genes for two chloroplast polypeptides in isolated pea leaf nuclei. EMBO J. 1982;1(12):1493–1498. doi: 10.1002/j.1460-2075.1982.tb01345.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Iino M., Schäfer E. Phototropic response of the stage I Phycomyces sporangiophore to a pulse of blue light. Proc Natl Acad Sci U S A. 1984 Nov;81(22):7103–7107. doi: 10.1073/pnas.81.22.7103. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kaufman L. S., Briggs W. R., Thompson W. F. Phytochrome control of specific mRNA levels in developing pea buds : the presence of both very low fluence and low fluence responses. Plant Physiol. 1985 Jun;78(2):388–393. doi: 10.1104/pp.78.2.388. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kaufman L. S., Roberts L. L., Briggs W. R., Thompson W. F. Phytochrome Control of Specific mRNA levels in Developing Pea Buds : Kinetics of Accumulation, Reciprocity, and Escape Kinetics of the Low Fluence Response. Plant Physiol. 1986 Aug;81(4):1033–1038. doi: 10.1104/pp.81.4.1033. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kaufman L. S., Thompson W. F., Briggs W. R. Different Red Light Requirements for Phytochrome-Induced Accumulation of cab RNA and rbcS RNA. Science. 1984 Dec 21;226(4681):1447–1449. doi: 10.1126/science.226.4681.1447. [DOI] [PubMed] [Google Scholar]
  11. Kaufman L. S., Watson J. C., Thompson W. F. Light-regulated changes in DNase I hypersensitive sites in the rRNA genes of Pisum sativum. Proc Natl Acad Sci U S A. 1987 Mar;84(6):1550–1554. doi: 10.1073/pnas.84.6.1550. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Manley J. L., Fire A., Samuels M., Sharp P. A. In vitro transcription: whole-cell extract. Methods Enzymol. 1983;101:568–582. doi: 10.1016/0076-6879(83)01038-1. [DOI] [PubMed] [Google Scholar]
  13. Mösinger E., Batschauer A., Schäfer E., Apel K. Phytochrome control of in vitro transcription of specific genes in isolated nuclei from barley (Hordeum vulgare). Eur J Biochem. 1985 Feb 15;147(1):137–142. doi: 10.1111/j.1432-1033.1985.tb08729.x. [DOI] [PubMed] [Google Scholar]
  14. Silverthorne J., Tobin E. M. Demonstration of transcriptional regulation of specific genes by phytochrome action. Proc Natl Acad Sci U S A. 1984 Feb;81(4):1112–1116. doi: 10.1073/pnas.81.4.1112. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Warpeha K. M., Kaufman L. S. Blue-Light Regulation of Epicotyl Elongation in Pisum sativum. Plant Physiol. 1989 Feb;89(2):544–548. doi: 10.1104/pp.89.2.544. [DOI] [PMC free article] [PubMed] [Google Scholar]

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