Skip to main content
. Author manuscript; available in PMC: 2011 Jun 20.
Published in final edited form as: Virology. 2010 Apr 7;402(1):94–101. doi: 10.1016/j.virol.2010.03.001

Figure 1. Minimal change in helicase activity of Rep78 in the presence of E1.

Figure 1

Standard helicase assay was performed in absence or presence of indicted amount of MBP-Rep78 and/or GST-E1 proteins. The helicase substrate consisted of a radiolabeled 17-mer annealed to M13mp18 ssDNA. (A) is one representative gel of helicase assay. The reaction mixture for the lane marked “No protein, boil” was heated to 100°C for 5 min before loading. (B) Quantitative estimation of helicase activity of the proteins. Each column represents the mean of three experiments and error bars indicate the standard error of each mean. Figure 1B is provided with a split X axis to allow easier reading of the experimental activity.