Abstract
Insertion sequence IS1 has two coding frames, insA and insB, which are essential for its transposition. Here, we show that a frameshifting event in the -1 direction from the 3' end region of the insA frame to an open reading frame (B' frame), extending from the 5' end of the insB frame, is involved in production of the InsA-B'-InsB fusion protein that has IS1 transposase activity. The frameshifting event is likely to have occurred at the sequence AAAAAC where the insA frame overlaps the B' frame. Interestingly, this sequence is also present in one of the two sequences identified in retroviruses as frameshift signals for production of transframe polyproteins from the overlapping genes gag-pro or gag-pro-pol.
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Selected References
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- Armstrong K. A., Ohtsubo H., Bauer W. R., Yoshioka Y., Miyazaki C., Maeda Y., Ohtsubo E. Characterization of the gene products produced in minicells by pSM1, a derivative of R100. Mol Gen Genet. 1986 Oct;205(1):56–65. doi: 10.1007/BF02428032. [DOI] [PubMed] [Google Scholar]
- Atkins J. F., Gesteland R. F., Reid B. R., Anderson C. W. Normal tRNAs promote ribosomal frameshifting. Cell. 1979 Dec;18(4):1119–1131. doi: 10.1016/0092-8674(79)90225-3. [DOI] [PubMed] [Google Scholar]
- Dayhuff T. J., Atkins J. F., Gesteland R. F. Characterization of ribosomal frameshift events by protein sequence analysis. J Biol Chem. 1986 Jun 5;261(16):7491–7500. [PubMed] [Google Scholar]
- Dunn J. J., Studier F. W. Complete nucleotide sequence of bacteriophage T7 DNA and the locations of T7 genetic elements. J Mol Biol. 1983 Jun 5;166(4):477–535. doi: 10.1016/s0022-2836(83)80282-4. [DOI] [PubMed] [Google Scholar]
- Fiandt M., Szybalski W., Malamy M. H. Polar mutations in lac, gal and phage lambda consist of a few IS-DNA sequences inserted with either orientation. Mol Gen Genet. 1972;119(3):223–231. doi: 10.1007/BF00333860. [DOI] [PubMed] [Google Scholar]
- Galas D. J., Calos M. P., Miller J. H. Sequence analysis of Tn9 insertions in the lacZ gene. J Mol Biol. 1980 Nov 25;144(1):19–41. doi: 10.1016/0022-2836(80)90213-2. [DOI] [PubMed] [Google Scholar]
- Gamas P., Galas D., Chandler M. DNA sequence at the end of IS1 required for transposition. Nature. 1985 Oct 3;317(6036):458–460. doi: 10.1038/317458a0. [DOI] [PubMed] [Google Scholar]
- Hashimoto-Gotoh T., Sekiguchi M. Mutations of temperature sensitivity in R plasmid pSC101. J Bacteriol. 1977 Aug;131(2):405–412. doi: 10.1128/jb.131.2.405-412.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hirsch H. J., Starlinger P., Brachet P. Two kinds of insertions in bacterial genes. Mol Gen Genet. 1972;119(3):191–206. doi: 10.1007/BF00333858. [DOI] [PubMed] [Google Scholar]
- Hizi A., Henderson L. E., Copeland T. D., Sowder R. C., Hixson C. V., Oroszlan S. Characterization of mouse mammary tumor virus gag-pro gene products and the ribosomal frameshift site by protein sequencing. Proc Natl Acad Sci U S A. 1987 Oct;84(20):7041–7045. doi: 10.1073/pnas.84.20.7041. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Iida S., Arber W. On the role of IS1 in the formation of hybrids between the bacteriophage P1 and the R plasmid NR1. Mol Gen Genet. 1980 Jan;177(2):261–270. doi: 10.1007/BF00267437. [DOI] [PubMed] [Google Scholar]
- Jacks T., Madhani H. D., Masiarz F. R., Varmus H. E. Signals for ribosomal frameshifting in the Rous sarcoma virus gag-pol region. Cell. 1988 Nov 4;55(3):447–458. doi: 10.1016/0092-8674(88)90031-1. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jacks T., Townsley K., Varmus H. E., Majors J. Two efficient ribosomal frameshifting events are required for synthesis of mouse mammary tumor virus gag-related polyproteins. Proc Natl Acad Sci U S A. 1987 Jun;84(12):4298–4302. doi: 10.1073/pnas.84.12.4298. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Johnsrud L. DNA sequence of the transposable element IS1. Mol Gen Genet. 1979 Jan 31;169(2):213–218. doi: 10.1007/BF00271673. [DOI] [PubMed] [Google Scholar]
- Luria S. E., Delbrück M. Mutations of Bacteria from Virus Sensitivity to Virus Resistance. Genetics. 1943 Nov;28(6):491–511. doi: 10.1093/genetics/28.6.491. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Machida C., Machida Y., Ohtsubo E. Both inverted repeat sequences located at the ends of IS1 provide promoter functions. J Mol Biol. 1984 Aug 5;177(2):247–267. doi: 10.1016/0022-2836(84)90455-8. [DOI] [PubMed] [Google Scholar]
- Machida C., Machida Y., Wang H. C., Ishizaki K., Ohtsubo E. Repression of cointegration ability of insertion element IS1 by transcriptional readthrough from flanking regions. Cell. 1983 Aug;34(1):135–142. doi: 10.1016/0092-8674(83)90143-5. [DOI] [PubMed] [Google Scholar]
- Machida Y., Machida C., Ohtsubo E. A novel type of transposon generated by insertion element IS102 present in a pSC101 derivative. Cell. 1982 Aug;30(1):29–36. doi: 10.1016/0092-8674(82)90008-3. [DOI] [PubMed] [Google Scholar]
- Machida Y., Machida C., Ohtsubo E. Insertion element IS1 encodes two structural genes required for its transposition. J Mol Biol. 1984 Aug 5;177(2):229–245. doi: 10.1016/0022-2836(84)90454-6. [DOI] [PubMed] [Google Scholar]
- Machida Y., Machida C., Ohtsubo H., Ohtsubo E. Factors determining frequency of plasmid cointegration mediated by insertion sequence IS1. Proc Natl Acad Sci U S A. 1982 Jan;79(2):277–281. doi: 10.1073/pnas.79.2.277. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Malamy M. H., Fiandt M., Szybalski W. Electron microscopy of polar insertions in the lac operon of Escherichia coli. Mol Gen Genet. 1972;119(3):207–222. doi: 10.1007/BF00333859. [DOI] [PubMed] [Google Scholar]
- Matsutani S., Ohtsubo H., Maeda Y., Ohtsubo E. Isolation and characterization of IS elements repeated in the bacterial chromosome. J Mol Biol. 1987 Aug 5;196(3):445–455. doi: 10.1016/0022-2836(87)90023-4. [DOI] [PubMed] [Google Scholar]
- Messing J. New M13 vectors for cloning. Methods Enzymol. 1983;101:20–78. doi: 10.1016/0076-6879(83)01005-8. [DOI] [PubMed] [Google Scholar]
- Moore R., Dixon M., Smith R., Peters G., Dickson C. Complete nucleotide sequence of a milk-transmitted mouse mammary tumor virus: two frameshift suppression events are required for translation of gag and pol. J Virol. 1987 Feb;61(2):480–490. doi: 10.1128/jvi.61.2.480-490.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nyman K., Nakamura K., Ohtsubo H., Ohtsubo E. Distribution of the insertion sequence IS1 in gram-negative bacteria. Nature. 1981 Feb 12;289(5798):609–612. doi: 10.1038/289609a0. [DOI] [PubMed] [Google Scholar]
- Nyman K., Ohtsubo H., Davison D., Ohtsubo E. Distribution of insertion element IS1 in natural isolates of Escherichia coli. Mol Gen Genet. 1983;189(3):516–518. doi: 10.1007/BF00325920. [DOI] [PubMed] [Google Scholar]
- Ohtsubo E., Zenilman M., Ohtsubo H., McCormick M., Machida C., Machida Y. Mechanism of insertion and cointegration mediated by IS1 and Tn3. Cold Spring Harb Symp Quant Biol. 1981;45(Pt 1):283–295. doi: 10.1101/sqb.1981.045.01.041. [DOI] [PubMed] [Google Scholar]
- Ohtsubo E., Zenilman M., Ohtsubo H. Plasmids containing insertion elements are potential transposons. Proc Natl Acad Sci U S A. 1980 Feb;77(2):750–754. doi: 10.1073/pnas.77.2.750. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ohtsubo H., Nyman K., Doroszkiewicz W., Ohtsubo E. Multiple copies of iso-insertion sequences of IS1 in Shigella dysenteriae chromosome. Nature. 1981 Aug 13;292(5824):640–643. doi: 10.1038/292640a0. [DOI] [PubMed] [Google Scholar]
- Ohtsubo H., Ohtsubo E. Nucleotide sequence of an insertion element, IS1. Proc Natl Acad Sci U S A. 1978 Feb;75(2):615–619. doi: 10.1073/pnas.75.2.615. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pabo C. O., Sauer R. T. Protein-DNA recognition. Annu Rev Biochem. 1984;53:293–321. doi: 10.1146/annurev.bi.53.070184.001453. [DOI] [PubMed] [Google Scholar]
- Rice N. R., Stephens R. M., Burny A., Gilden R. V. The gag and pol genes of bovine leukemia virus: nucleotide sequence and analysis. Virology. 1985 Apr 30;142(2):357–377. doi: 10.1016/0042-6822(85)90344-7. [DOI] [PubMed] [Google Scholar]
- Saedler H., Heiss B. Multiple copies of the insertion-DNA sequences IS1 and IS2 in the chromosome of E. coli K-12. Mol Gen Genet. 1973 May 9;122(3):267–277. doi: 10.1007/BF00278602. [DOI] [PubMed] [Google Scholar]
- Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shimotohno K., Takahashi Y., Shimizu N., Gojobori T., Golde D. W., Chen I. S., Miwa M., Sugimura T. Complete nucleotide sequence of an infectious clone of human T-cell leukemia virus type II: an open reading frame for the protease gene. Proc Natl Acad Sci U S A. 1985 May;82(10):3101–3105. doi: 10.1073/pnas.82.10.3101. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yanisch-Perron C., Vieira J., Messing J. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 1985;33(1):103–119. doi: 10.1016/0378-1119(85)90120-9. [DOI] [PubMed] [Google Scholar]
- Yoshinaka Y., Katoh I., Copeland T. D., Smythers G. W., Oroszlan S. Bovine leukemia virus protease: purification, chemical analysis, and in vitro processing of gag precursor polyproteins. J Virol. 1986 Mar;57(3):826–832. doi: 10.1128/jvi.57.3.826-832.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yoshioka Y., Ohtsubo H., Ohtsubo E. Repressor gene finO in plasmids R100 and F: constitutive transfer of plasmid F is caused by insertion of IS3 into F finO. J Bacteriol. 1987 Feb;169(2):619–623. doi: 10.1128/jb.169.2.619-623.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zerbib D., Jakowec M., Prentki P., Galas D. J., Chandler M. Expression of proteins essential for IS1 transposition: specific binding of InsA to the ends of IS1. EMBO J. 1987 Oct;6(10):3163–3169. doi: 10.1002/j.1460-2075.1987.tb02627.x. [DOI] [PMC free article] [PubMed] [Google Scholar]