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. 1989 Jun;86(12):4649–4653. doi: 10.1073/pnas.86.12.4649

Relative contribution of "determinant selection" and "holes in the T-cell repertoire" to T-cell responses.

E B Schaeffer 1, A Sette 1, D L Johnson 1, M C Bekoff 1, J A Smith 1, H M Grey 1, S Buus 1
PMCID: PMC287328  PMID: 2471972

Abstract

Using BALB/c and CBA/J mice, the I-region associated (Ia) binding capacity and T-cell immunogenicity of a panel of 14 overlapping peptides that span the entire sequence of the protein staphylococcal nuclease (Nase) was examined to evaluate major histocompatibility gene complex (MHC) control of T-cell responses. Ia binding and Ia-restricted T-cell immunogenicity could be determined for a total of 54 peptide-MHC combinations. Only 30% of the 54 instances examined involved detectable Ia binding, but they represented almost all (12 of 13) of the immune responses found. However, binding to Ia was not sufficient to ensure T-cell immunogenicity, since only 70% of the binding events were productive--i.e., were associated with an immune response. Thus, Ia molecules have the expected characteristics of a highly permissive capacity for antigen interaction that allows them to function as restriction elements for a large universe of antigens. On the other hand, since the Ia molecules cannot distinguish between self and non-self, not all antigen-Ia interactions would be permitted to elicit a T-cell response. It appears that both Ia binding ("determinant selection") and T-cell repertoire act in concert to define the immune response status of an individual toward any particular T-cell epitope.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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