Abstract
The bacterial transposon Tn7 encodes two distinct but overlapping transposition pathways. tnsABC + tnsD promote transposition to a specific site, attTn7, while tnsABC + tnsE promote transposition to many other sites unrelated to attTn7. We have identified a tnsD-dependent DNA binding activity that specifically recognizes attTn7. We have localized the recognition sequences for this activity to a 28-base-pair region and have shown that this same region can provide specific properties of an attTn7 target in vivo. Interestingly, these sequences are positioned more than 25 base pairs from the specific point of Tn7 insertion.
Full text
PDFImages in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Barth P. T., Datta N., Hedges R. W., Grinter N. J. Transposition of a deoxyribonucleic acid sequence encoding trimethoprim and streptomycin resistances from R483 to other replicons. J Bacteriol. 1976 Mar;125(3):800–810. doi: 10.1128/jb.125.3.800-810.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bastos M. C., Murphy E. Transposon Tn554 encodes three products required for transposition. EMBO J. 1988 Sep;7(9):2935–2941. doi: 10.1002/j.1460-2075.1988.tb03152.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1006/abio.1976.9999. [DOI] [PubMed] [Google Scholar]
- Craigie R., Arndt-Jovin D. J., Mizuuchi K. A defined system for the DNA strand-transfer reaction at the initiation of bacteriophage Mu transposition: protein and DNA substrate requirements. Proc Natl Acad Sci U S A. 1985 Nov;82(22):7570–7574. doi: 10.1073/pnas.82.22.7570. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fried M., Crothers D. M. Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis. Nucleic Acids Res. 1981 Dec 11;9(23):6505–6525. doi: 10.1093/nar/9.23.6505. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Garner M. M., Revzin A. A gel electrophoresis method for quantifying the binding of proteins to specific DNA regions: application to components of the Escherichia coli lactose operon regulatory system. Nucleic Acids Res. 1981 Jul 10;9(13):3047–3060. doi: 10.1093/nar/9.13.3047. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gay N. J., Tybulewicz V. L., Walker J. E. Insertion of transposon Tn7 into the Escherichia coli glmS transcriptional terminator. Biochem J. 1986 Feb 15;234(1):111–117. doi: 10.1042/bj2340111. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gringauz E., Orle K. A., Waddell C. S., Craig N. L. Recognition of Escherichia coli attTn7 by transposon Tn7: lack of specific sequence requirements at the point of Tn7 insertion. J Bacteriol. 1988 Jun;170(6):2832–2840. doi: 10.1128/jb.170.6.2832-2840.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hanahan D. Studies on transformation of Escherichia coli with plasmids. J Mol Biol. 1983 Jun 5;166(4):557–580. doi: 10.1016/s0022-2836(83)80284-8. [DOI] [PubMed] [Google Scholar]
- Lichtenstein C., Brenner S. Site-specific properties of Tn7 transposition into the E. coli chromosome. Mol Gen Genet. 1981;183(2):380–387. doi: 10.1007/BF00270644. [DOI] [PubMed] [Google Scholar]
- Lichtenstein C., Brenner S. Unique insertion site of Tn7 in the E. coli chromosome. Nature. 1982 Jun 17;297(5867):601–603. doi: 10.1038/297601a0. [DOI] [PubMed] [Google Scholar]
- McKown R. L., Orle K. A., Chen T., Craig N. L. Sequence requirements of Escherichia coli attTn7, a specific site of transposon Tn7 insertion. J Bacteriol. 1988 Jan;170(1):352–358. doi: 10.1128/jb.170.1.352-358.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McKown R. L., Waddell C. S., Arciszewska L. K., Craig N. L. Identification of a transposon Tn7-dependent DNA-binding activity that recognizes the ends of Tn7. Proc Natl Acad Sci U S A. 1987 Nov;84(22):7807–7811. doi: 10.1073/pnas.84.22.7807. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mizuuchi M., Mizuuchi K. The extent of DNA sequence required for a functional bacterial attachment site of phage lambda. Nucleic Acids Res. 1985 Feb 25;13(4):1193–1208. doi: 10.1093/nar/13.4.1193. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Morisato D., Kleckner N. Tn10 transposition and circle formation in vitro. Cell. 1987 Oct 9;51(1):101–111. doi: 10.1016/0092-8674(87)90014-6. [DOI] [PubMed] [Google Scholar]
- Murphy E., Löfdahl S. Transposition of Tn554 does not generate a target duplication. Nature. 1984 Jan 19;307(5948):292–294. doi: 10.1038/307292a0. [DOI] [PubMed] [Google Scholar]
- Pabo C. O., Sauer R. T. Protein-DNA recognition. Annu Rev Biochem. 1984;53:293–321. doi: 10.1146/annurev.bi.53.070184.001453. [DOI] [PubMed] [Google Scholar]
- Rogers M., Ekaterinaki N., Nimmo E., Sherratt D. Analysis of Tn7 transposition. Mol Gen Genet. 1986 Dec;205(3):550–556. doi: 10.1007/BF00338097. [DOI] [PubMed] [Google Scholar]
- Singh H., Sen R., Baltimore D., Sharp P. A. A nuclear factor that binds to a conserved sequence motif in transcriptional control elements of immunoglobulin genes. Nature. 1986 Jan 9;319(6049):154–158. doi: 10.1038/319154a0. [DOI] [PubMed] [Google Scholar]
- Struhl K. Direct selection for gene replacement events in yeast. Gene. 1983 Dec;26(2-3):231–241. doi: 10.1016/0378-1119(83)90193-2. [DOI] [PubMed] [Google Scholar]
- Waddell C. S., Craig N. L. Tn7 transposition: two transposition pathways directed by five Tn7-encoded genes. Genes Dev. 1988 Feb;2(2):137–149. doi: 10.1101/gad.2.2.137. [DOI] [PubMed] [Google Scholar]
- Walker J. E., Gay N. J., Saraste M., Eberle A. N. DNA sequence around the Escherichia coli unc operon. Completion of the sequence of a 17 kilobase segment containing asnA, oriC, unc, glmS and phoS. Biochem J. 1984 Dec 15;224(3):799–815. doi: 10.1042/bj2240799. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yuan R. Structure and mechanism of multifunctional restriction endonucleases. Annu Rev Biochem. 1981;50:285–319. doi: 10.1146/annurev.bi.50.070181.001441. [DOI] [PubMed] [Google Scholar]