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. 1989 Jun;86(11):4302–4305. doi: 10.1073/pnas.86.11.4302

Rapid increase in enzyme and peptide mRNA in sympathetic ganglia after electrical stimulation in humans.

M Schalling 1, P E Stieg 1, C Lindquist 1, M Goldstein 1, T Hökfelt 1
PMCID: PMC287440  PMID: 2567003

Abstract

Thoracic ganglia in humans were studied after electrical, preganglionic stimulation using in situ hybridization with synthetic oligonucleotide probes against the catecholamine-synthesizing enzymes tyrosine hydroxylase (EC 1.14.16.2) and dopamine beta-hydroxylase (EC 1.14.17.1) and neuropeptide tyrosine. Immunohistochemical analysis was also performed. Following short peroperative stimulation a severalfold increase in all three mRNAs was found in principal ganglion cells, whereas no definite changes could be detected in enzyme or peptide levels with immunohistochemistry. The results suggest a very rapid and sensitive regulation of genes involved in signal transmission in the sympathetic nervous system of humans. Moreover, they indicate that electrical stimulation of neurons and/or pathways combined with in situ hybridization may be used as a method to define neuronal projections by visualizing increases in mRNAs for transmitter enzymes and/or peptide in target cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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