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. Author manuscript; available in PMC: 2010 May 23.
Published in final edited form as: AIDS. 2010 Mar 13;24(5):659–667. doi: 10.1097/QAD.0b013e328336781d

Figure 1. ATP-mediated AZT-MP excision activity of WT, K70R, Y181C, K70R/Y181C and K70R/Y181C/N348I HIV-1 RT on RNA/DNA and DNA/DNA T/Ps.

Figure 1

A) Schematic representation of the ATP-mediated excision assay used in this study. Experimental details are provided in the Materials and Methods. AZT-MP is designated as “Z”

B) Representative autoradiogram of AZT-MP excision reactions carried out by WT and mutant RT on RNA/DNA and DNA/DNA T/Ps. The reaction times were 3, 5, 10, 15, 30, 60 and 90 min.

C) Isotherms of ATP-mediated AZT-MP excision reactions carried out by WT and mutant HIV-1 RT on a DNA/DNA T/P (left-hand curve) and on an RNA/DNA T/P (right-hand curve). Data are the mean ± standard deviation from at least three independent experiments.