Table 1.
Identification of the human AKR1C1 basal proximal promoter.
| Fold increase in relative luciferase activity (RLA) | |||||
|---|---|---|---|---|---|
| 5’ Deletion Constructs | HepG2 | 2008 | 2008/C13* | H23 | A549 |
| pGL3 Easic | 1 | 1 | 1 | 1 | 1 |
| pAKRICI −88/+59 | 1.1 ± 0.2 | 1.4 ± 0.4 | 1.3 ± 0.8 | 1 ± 0.3 | 2.8 ± 1 |
| pAKRICI −128/+59 | 4.5 ± 2.8 | 8.2 ± 0.6 | 3.3 ± 0.6 | 7.7 ± 1.0 | 22.0 ± 9.8 |
| pAKRICI −152/+59 | 6.6 ± 2.0 | - | - | - | - |
| pAKRICI −180/+59 | 7.1 ± 2.5 | 10.5 ± 3.6 | 7.3 ± 1.0 | 10.7 ± 1.5 | 17.8 ± 1.7 |
| pAKRICI −349/+59 | 8.8 ± 3.2 | - | - | - | - |
| pAKRICI −641/+59 | 11.2 ± 6.5 | 13.7 ± 2.6 | 11 ± 3.0 | 15.8 ± 3.0 | 33 ± 3.8 |
| pAKRICI −1648/+59 | 11.0 ± 3.0 | - | - | - | - |
| pAKRICI −2301/+59 | 6.7 ± 1.6 | - | - | - | - |
| pAKRICI −2567Z+59 | 6.5 ± 1.7 | - | - | - | - |
| pAKRICI −2954/459 | 5.8 ± 1.8 | 13.5 ± 8.0 | 22.9 ± 8.5 | 36.4 ± 19.2 | 29.2 ± 1.8 |
Human liver carcinoma cells (HepG2), ovarian carcinoma cells (2008, 2008/C13*) were transfected with various AKR1C1 promoters constructs shown in Fig 1B and assayed for luciferase activity after 48h as described in Materials and Methods. Fold increase in relative luciferase activity (RLA) was compared with that observed with pGL3 basic (set as 1). Normalization in transfection efficiency was performed by co-transfection with pRL-SV40 (Renilla expression vector). The mean ± S.D are from three different experiments, each performed in triplicate.