Table 2.

List of primers utilized for RT-PCR analysis

Gene	Forward	Reverse	Annealing temperature
GAPDH	5’-acccactcctccacctttg-3’	5’-ctcttgtgctcttgctggg-3’	55°C
AKR1C1	5’-gtaaagctttagaggccac-3’	5’-ataaggtagaggtcaacataa-3’	55°C
NFYA	5’-gtccagaccctccaggtagt-3’	5’-gggaccaactgtatttgctg-3’	58°C

GAPDH- Glyceraldehyde-6-phosphate dehydrogenase; AKR1C1- Aldo keto-reductase 1C1; NF-YA- Nuclear factor Y alpha. The PCR was performed with an initial denaturation at 95°C for 15 mins, followed by 45 cycles of 94°C for 15 s, annealing for 30s and extension at 72°C for 30s.