Table 2.
Gene | Forward | Reverse | Annealing temperature |
---|---|---|---|
GAPDH | 5’-acccactcctccacctttg-3’ | 5’-ctcttgtgctcttgctggg-3’ | 55°C |
AKR1C1 | 5’-gtaaagctttagaggccac-3’ | 5’-ataaggtagaggtcaacataa-3’ | 55°C |
NFYA | 5’-gtccagaccctccaggtagt-3’ | 5’-gggaccaactgtatttgctg-3’ | 58°C |
GAPDH- Glyceraldehyde-6-phosphate dehydrogenase; AKR1C1- Aldo keto-reductase 1C1; NF-YA- Nuclear factor Y alpha. The PCR was performed with an initial denaturation at 95°C for 15 mins, followed by 45 cycles of 94°C for 15 s, annealing for 30s and extension at 72°C for 30s.