Table 3.
Ontologies indentified by GeneSifter that change with loss of Meg3
| Ontology | No. of genes with increased expression in KO embryos | No. of genes with decreased expression in KO embryos | Z-score (up) | Z-score (down) |
|---|---|---|---|---|
| VEGF signaling | 3 | 0 | 4.19 | <2 |
| Neuropeptide signaling | 3 | 2 | 3.95 | <2 |
| Blood vessel patterning | 3 | 1 | 3.33 | 3.88 |
| Axon extension | 3 | 0 | 3.21 | <2 |
| Circadian rhythm | 3 | 0 | 2.92 | <2 |
| Homophilic cell adhesion | 6 | 0 | 2.75 | <2 |
| Voltage-gated channel activity | 10 | 0 | 2.68 | <2 |
| GTPase activator activity | 10 | 0 | 2.60 | <2 |
| Transcription | 67 | 5 | 2.54 | <2 |
| Calcium binding | 30 | 1 | 2.40 | <2 |
| Gated channel activity | 13 | 0 | 2.40 | <2 |
| Kinase activity | 32 | 3 | 2.31 | <2 |
| DNA repair | 10 | 2 | 2.25 | <2 |
| Regulation of neuronal action potential | 3 | 0 | 2.25 | <2 |
| Actin cytoskeletal organization and biogenesis | 9 | 0 | 2.24 | <2 |
| Embryonic pattern specification | 3 | 1 | <2 | 2.50 |
| Postembryonic development | 2 | 1 | <2 | 2.30 |
| Cellular lipid metabolic process | 14 | 5 | <2 | 3.08 |
Biological process or molecular function ontologies with a Z-score >2 and three or more genes that change with loss of Meg3 were considered significant. A subset of these ontologies that are relevant for development, tumorigenesis, and/or physiological functions are listed here. KO, Knockout.