Table 4.

Changes in microvessel density visualized by Pecam1 staining with loss of Meg3

Litter	Average microvessel density (WT)	Average microvessel density (KO)	Percent change
1	72.10	123.95	41.83
2	75.52	121.90	38.05
3	78.92	113.06	30.20
4	67.27	81.73	17.69
5	72.91	86.09	15.31
All	73.34	105.35	30.38

Microvessels were visualized in 5-μm cortical sections from seven Meg3-null embryos and nine wild-type (WT) litter mate controls from five litters by Pecam staining. Microvessel number in three cortical fields per embryo was counted blindly and independently before sample identification codes were released. KO, Knockout.