. 2010 May;51(1):56–65. doi: 10.1016/j.ymeth.2010.01.035

Table 2.

Primers for cloning genomic fragments for in vitro transcription of RNA used as standard for qRT-PCR.

Fragment	Direction	Sequence (5′–3′)^a	Amplicon (kb)
Nanog	Forward	CTCCTAATACGACTCACTATAGGGTGCCTCTTCAAGGCAGCCCTGATTCT	2
Nanog	Reverse	GTAGAATTAACCCTCACTAAAGGGTTGTGGGGTGCTAAAATGCGCATGGC	2
Oct4	Forward	AGGATAATACGACTCACTATAGGGAGCTAGAACAGTTTGCCAAGCTGCTG	1.4
Oct4	Reverse	CTGGTGAATTAACCCTCACTAAAGGGAGAGCCCAGAGCAGTGACGGGAAC	1.4
Sox2	Forward	GCCCGCTAATACGACTCACTATAGGGATGATGGAGACGGAGCTGAAGCC	2.1
Sox2	Reverse	TGTCAATTAACCCTCACTAAAGGGATCTCAAACTGTGCATAATGGAGTAA	2.1
G3PDH	Forward	CAGCTCTAATACGACTCACTATAGGGATTTGGCCGTATTGGGCGCCTGG	1.4
G3PDH	Reverse	CTTGAATTAACCCTCACTAAAGGGTTTCTTACTCCTTGGAGGCCATGTAG	1.4
c-Jun	Forward	CGCTAATACGACTCACTATAGGGATGACTGCAAAGATGGAAACGACCTTC	1.1
c-Jun	Reverse	TTGCAATTAACCCTCACTAAAGGGTTCTCAAAACGTTTGCAACTGCTGCG	1.1
VegT	Forward	GTGATATAATACGACTCACTATAGGGAATGAGAAACTGCTGTCGGGAATG	1.4
VegT	Reverse	GTGGATAATTAACCCTCACTAAAGGGTTACCAACAGCTGTAGGGGAAGAG	1.4

T7 and T3 RNA promoter sequences are shown in bold. T7 is used on the forward primer for synthesis of in vitro RNA that mimics a portion of the corresponding mouse primary transcript.