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. 2010 Jun;130(2):243–253. doi: 10.1111/j.1365-2567.2009.03230.x

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Journal compilation © 2010 Blackwell Publishing Ltd

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Flow cytometric analyses of HSA/CD27 expression in the double-negative (DN) and double-positive (DP) fractions. Thymocytes were prepared from Lck-Cre-tg and distal Runx1-tg;Lck-Cre-tg mice, and processed for flow cytometric analyses of CD4, CD8 and HSA/CD27. The DN-gated and DP-gated fractions were analysed for their fluorescence intensities of HSA (a) and CD27 (b). In (a), the percentages of HSA^hi and HSA^lo sub-fractions and the mean fluorescence intensities of HSA are indicated, whereas the numbers seen in (b) indicate the percentages of CD27^med/hi sub-fractions. Note that the percentage of CD27^med/hi is assumed to be 100 for the DN fractions in (b).