Table 2.

Effect of invariant natural killer T-cell blockade on the proportion of splenic dendritic cells and macrophages

	CD11c+ CD11b−	CD11c+ CD11b+	Macrophages (CD14+)
Non-immunized	2·03 ± 0·32	4·64 ± 0·33	0·87 ± 0·14
CII/CFA	0·72 ± 0·23****	2·04 ± 0·36****	0·47 ± 0·04***
CII/CFA+ anti-CD1d	0·64 ± 0·17‡‡‡‡	1·82 ± 0·26‡‡‡‡	0·41 ± 0·05‡‡‡

Three groups of mice were used: mice immunized on day 0 with collagen II in complete Freund’s adjuvant (CII/CFA, n = 6), mice immunized with CII/CFA and given 200 μg of anti-CD1d intraperitoneally on days 0 and 3 (n = 6), and non-immunized mice as controls (n = 6). Spleen cells were collected from all mice (on day 6) and labelled with fluorochrome-conjugated anti-CD11c, anti-CD11b and anti-CD14 for analysis by flow cytometry. The percentage of CD11c⁺ CD11b⁻ and CD11c⁺CD11b⁺ dendritic cells and of CD14⁺ macrophages are given as mean values ± SEM. Results are expressed as % (mean ± SEM) among all splenocytes and mean fluorescence intensity (MFI ± SEM).

Non-immunized versus CII/CFA:

^***P< 0·01;

^****P< 0·001. CII/CFA + anti-CD1d versus non-immunized:

^‡‡‡P< 0·01;

^‡‡‡‡P< 0·001. (Student’s t-test).