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. Author manuscript; available in PMC: 2010 Dec 3.
Published in final edited form as: Oncogene. 2010 Apr 12;29(22):3208–3216. doi: 10.1038/onc.2010.103

Figure 2. EGF, heregulin, Gas6, bombesin, and IL-6 stimulate proliferation of androgen-dependent prostate cancer cell lines in the absence of androgen.

Figure 2

Cells were seeded in serum-free medium in triplicate wells of a 96-well plate and treated with EGF (100 ng/ml), heregulin (10 ng/ml), Gas6 (100 ng/ml), IL-6 (10 ng/ml), or bombesin (1 nM) for 72 hours. Relative cell proliferation was determined by addition of colorimetric dye WST-8 and measuring absorbance at 450 nM. (A) LNCaP cells. (B) LAPC-4 cells. Data shown are representative of three similar independent experiments. All treatment conditions are significantly different from untreated control with p <0.05 by t-test.