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. Author manuscript; available in PMC: 2010 Jun 8.
Published in final edited form as: Biochemistry. 2010 May 18;49(19):4116–4125. doi: 10.1021/bi100102s

Figure 3.

Figure 3

Kinetics of dAMP insertion opposite 8-oxoG by the Y567A mutant (A and B) and the wt (C and D). A. Progress curves with various [dATP], 10, 40, 100, 250, 700, and 1500 µM (bottom to top), fit to single exponential equations. 1 mg/ml Heparin was used to prevent rebinding of the enzyme to the P/T. B. Plot of kobs versus [dATP] fit to Equation 2 to obtain kpol and Kd,app. C. Same as A but with the wt. Progress curves were obtained without a heparin trap. D. Same as B using the results of C.