. Author manuscript; available in PMC: 2010 Jun 8.

Published in final edited form as: Biochemistry. 2010 May 18;49(19):4116–4125. doi: 10.1021/bi100102s

Table 4.

Kinetic parameters for insertion of correct dNMPs past C:8oxoG and A:8oxoG terminal base-pairs.

		wt RB69pol			Y567A
dNTP	P/T	k_pol (s⁻¹)	K_d,app (µM)	k_pol/K_d,app (µM⁻¹ s⁻¹)	K_pol (s⁻¹)	K_d,app (µM)	k_pol/K_d,app (µ M⁻¹ s⁻¹)
dTTP	D_C:OG	ND^a	ND^a	0.0050^b	230	740	0.31
	D_A:OG	ND^a	ND^a	0.012 ^b	200	850	0.24

ND = Not Determined because K_d,app values were too high (> 2 mM) (see Fig. 6).

See (^d) from Table 2: data from Fig. 6 was fitted to a linear equation to obtain k_pol/K_d,app. Standard deviations were within the same range as the data presented in Table 2.