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. 1993 Oct;92(4):1819–1823. doi: 10.1172/JCI116772

Effects of psychosocial stress on endothelium-mediated dilation of atherosclerotic arteries in cynomolgus monkeys.

J K Williams 1, J R Kaplan 1, S B Manuck 1
PMCID: PMC288345  PMID: 8408634

Abstract

The objectives of this study were to determine if psychosocial stress impairs dilation through endothelium-derived relaxing factor (EDRF)-mediated mechanisms and if this effect is long lasting. Monkeys were fed an atherogenic diet for 36 mo while in one of three experimental conditions: (a) stable social groups ("unstressed," n = 6); (b) unstable social groups for the first half of the experiment and stable groups for the second half ("early stress," n = 8); and (c) stable groups for the first half of the experiment and unstable groups for the second half ("late stress," n = 6). Iliac arteries were studied in organ chambers containing Krebs' buffer and 10(-6) M indomethacin. Arteries from the late stress group had impaired dilation (shift of the dose-response curve down and to the right) to acetylcholine and the calcium ionophore A23187 (for both, P < 0.05), but not to nitroprusside (P > 0.05), compared with unstressed or early stress monkeys. NG-methyl-L-arginine reduced the dose-response curve to both acetylcholine and A23187 in the unstressed group and resulted in similar vascular responses among all three groups (P > 0.05). We conclude that current, but not previous, exposure to chronic stress impairs endothelium-mediated dilation of atherosclerotic iliac arteries of cynomolgus monkeys through an EDRF-mediated mechanism.

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Selected References

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  1. Allain C. C., Poon L. S., Chan C. S., Richmond W., Fu P. C. Enzymatic determination of total serum cholesterol. Clin Chem. 1974 Apr;20(4):470–475. [PubMed] [Google Scholar]
  2. Cooke J. P., Rossitch E., Jr, Andon N. A., Loscalzo J., Dzau V. J. Flow activates an endothelial potassium channel to release an endogenous nitrovasodilator. J Clin Invest. 1991 Nov;88(5):1663–1671. doi: 10.1172/JCI115481. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Corbett W. T., Schey H. M., Lehner N. D., Greene A. W. Standardized method for recording blood pressure in anaesthetized Macaca fascicularis. Lab Anim. 1981 Jan;15(1):37–40. doi: 10.1258/002367781780958621. [DOI] [PubMed] [Google Scholar]
  4. Dimsdale J. E., Moss J. Plasma catecholamines in stress and exercise. JAMA. 1980 Jan 25;243(4):340–342. [PubMed] [Google Scholar]
  5. Dimsdale J. E., Ziegler M., Mills P. Renin correlates with blood pressure reactivity to stressors. Neuropsychopharmacology. 1990 Aug;3(4):237–242. [PubMed] [Google Scholar]
  6. Furchgott R. F., Zawadzki J. V. The obligatory role of endothelial cells in the relaxation of arterial smooth muscle by acetylcholine. Nature. 1980 Nov 27;288(5789):373–376. doi: 10.1038/288373a0. [DOI] [PubMed] [Google Scholar]
  7. Heistad D. D., Armstrong M. L., Marcus M. L., Piegors D. J., Mark A. L. Augmented responses to vasoconstrictor stimuli in hypercholesterolemic and atherosclerotic monkeys. Circ Res. 1984 Jun;54(6):711–718. doi: 10.1161/01.res.54.6.711. [DOI] [PubMed] [Google Scholar]
  8. Kaplan J. R., Manuck S. B., Clarkson T. B., Lusso F. M., Taub D. M. Social status, environment, and atherosclerosis in cynomolgus monkeys. Arteriosclerosis. 1982 Sep-Oct;2(5):359–368. doi: 10.1161/01.atv.2.5.359. [DOI] [PubMed] [Google Scholar]
  9. Ludmer P. L., Selwyn A. P., Shook T. L., Wayne R. R., Mudge G. H., Alexander R. W., Ganz P. Paradoxical vasoconstriction induced by acetylcholine in atherosclerotic coronary arteries. N Engl J Med. 1986 Oct 23;315(17):1046–1051. doi: 10.1056/NEJM198610233151702. [DOI] [PubMed] [Google Scholar]
  10. Manuck S. B., Kaplan J. R., Clarkson T. B. Behaviorally induced heart rate reactivity and atherosclerosis in cynomolgus monkeys. Psychosom Med. 1983 May;45(2):95–108. doi: 10.1097/00006842-198305000-00002. [DOI] [PubMed] [Google Scholar]
  11. Manuck S. B., Kaplan J. R., Matthews K. A. Behavioral antecedents of coronary heart disease and atherosclerosis. Arteriosclerosis. 1986 Jan-Feb;6(1):2–14. doi: 10.1161/01.atv.6.1.2. [DOI] [PubMed] [Google Scholar]
  12. Maseri A., L'Abbate A., Baroldi G., Chierchia S., Marzilli M., Ballestra A. M., Severi S., Parodi O., Biagini A., Distante A. Coronary vasospasm as a possible cause of myocardial infarction. A conclusion derived from the study of "preinfarction" angina. N Engl J Med. 1978 Dec 7;299(23):1271–1277. doi: 10.1056/NEJM197812072992303. [DOI] [PubMed] [Google Scholar]
  13. Maseri A., Severi S., Nes M. D., L'Abbate A., Chierchia S., Marzilli M., Ballestra A. M., Parodi O., Biagini A., Distante A. "Variant" angina: one aspect of a continuous spectrum of vasospastic myocardial ischemia. Pathogenetic mechanisms, estimated incidence and clinical and coronary arteriographic findings in 138 patients. Am J Cardiol. 1978 Dec;42(6):1019–1035. doi: 10.1016/0002-9149(78)90691-4. [DOI] [PubMed] [Google Scholar]
  14. Moncada S., Higgs E. A. Endogenous nitric oxide: physiology, pathology and clinical relevance. Eur J Clin Invest. 1991 Aug;21(4):361–374. doi: 10.1111/j.1365-2362.1991.tb01383.x. [DOI] [PubMed] [Google Scholar]
  15. Myers P. R., Minor R. L., Jr, Guerra R., Jr, Bates J. N., Harrison D. G. Vasorelaxant properties of the endothelium-derived relaxing factor more closely resemble S-nitrosocysteine than nitric oxide. Nature. 1990 May 10;345(6271):161–163. doi: 10.1038/345161a0. [DOI] [PubMed] [Google Scholar]
  16. Omar H. A., Cherry P. D., Mortelliti M. P., Burke-Wolin T., Wolin M. S. Inhibition of coronary artery superoxide dismutase attenuates endothelium-dependent and -independent nitrovasodilator relaxation. Circ Res. 1991 Sep;69(3):601–608. doi: 10.1161/01.res.69.3.601. [DOI] [PubMed] [Google Scholar]
  17. Rozanski A., Bairey C. N., Krantz D. S., Friedman J., Resser K. J., Morell M., Hilton-Chalfen S., Hestrin L., Bietendorf J., Berman D. S. Mental stress and the induction of silent myocardial ischemia in patients with coronary artery disease. N Engl J Med. 1988 Apr 21;318(16):1005–1012. doi: 10.1056/NEJM198804213181601. [DOI] [PubMed] [Google Scholar]
  18. Sherman C. T., Litvack F., Grundfest W., Lee M., Hickey A., Chaux A., Kass R., Blanche C., Matloff J., Morgenstern L. Coronary angioscopy in patients with unstable angina pectoris. N Engl J Med. 1986 Oct 9;315(15):913–919. doi: 10.1056/NEJM198610093151501. [DOI] [PubMed] [Google Scholar]
  19. Strawn W. B., Bondjers G., Kaplan J. R., Manuck S. B., Schwenke D. C., Hansson G. K., Shively C. A., Clarkson T. B. Endothelial dysfunction in response to psychosocial stress in monkeys. Circ Res. 1991 May;68(5):1270–1279. doi: 10.1161/01.res.68.5.1270. [DOI] [PubMed] [Google Scholar]
  20. Williams J. K., Vita J. A., Manuck S. B., Selwyn A. P., Kaplan J. R. Psychosocial factors impair vascular responses of coronary arteries. Circulation. 1991 Nov;84(5):2146–2153. doi: 10.1161/01.cir.84.5.2146. [DOI] [PubMed] [Google Scholar]
  21. Yeung A. C., Vekshtein V. I., Krantz D. S., Vita J. A., Ryan T. J., Jr, Ganz P., Selwyn A. P. The effect of atherosclerosis on the vasomotor response of coronary arteries to mental stress. N Engl J Med. 1991 Nov 28;325(22):1551–1556. doi: 10.1056/NEJM199111283252205. [DOI] [PubMed] [Google Scholar]
  22. de Waal F. B. The organization of agonistic relations within two captive groups of Java-monkeys (Macaca fascicularis). Z Tierpsychol. 1977 Jul;44(3):225–282. doi: 10.1111/j.1439-0310.1977.tb00995.x. [DOI] [PubMed] [Google Scholar]

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