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. 1993 Nov;92(5):2339–2345. doi: 10.1172/JCI116838

Different localization and regulation of two types of vasopressin receptor messenger RNA in microdissected rat nephron segments using reverse transcription polymerase chain reaction.

Y Terada 1, K Tomita 1, H Nonoguchi 1, T Yang 1, F Marumo 1
PMCID: PMC288415  PMID: 8227349

Abstract

Recent studies have revealed that arginine vasopressin (AVP) has at least two types of receptors in the kidney: V1a receptor and V2 receptor. In this study, microlocalization of mRNA coding for V1a and V2 receptors was carried out in the rat kidney using a reverse transcription and polymerase chain reaction. Large signals for V1a receptor PCR product were detected in the glomerulus, initial cortical collecting duct, cortical collecting duct, outer medullary collecting duct, inner medullary collecting duct, and arcuate artery. Small but detectable signals were found in proximal convoluted and straight tubules, inner medullary thin limbs, and medullary thick ascending limbs. Large signals for V2 receptor mRNA were detected in the cortical collecting duct, outer medullary collecting duct, and inner medullary collecting duct. Small signals for V2 receptor were found in the inner medullary thick limbs, medullary thick ascending limbs, and initial cortical collecting duct. Next, we investigated V1a and V2 receptor mRNA regulation in the dehydrated state. During a 72-h water restriction state, the plasma AVP level increased and V2 receptor mRNA decreased in collecting ducts. In contrast, V1a receptor mRNA did not change significantly. Thus, the two AVP receptor subtypes are distributed differently along the nephron, and these mRNAs are regulated differently in the dehydrated state.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ammar A., Roseau S., Butlen D. Pharmacological characterization of V1a vasopressin receptors in the rat cortical collecting duct. Am J Physiol. 1992 Apr;262(4 Pt 2):F546–F553. doi: 10.1152/ajprenal.1992.262.4.F546. [DOI] [PubMed] [Google Scholar]
  2. Ammar A., Schmidt A., Semmekrot B., Roseau S., Butlen D. Receptors for neurohypophyseal hormones along the rat nephron: 125I-labelled d(CH2)5[Tyr(Me)2, Thr4, Orn8, Tyr-NH(2)9] vasotocin binding in microdissected tubules. Pflugers Arch. 1991 Apr;418(3):220–227. doi: 10.1007/BF00370519. [DOI] [PubMed] [Google Scholar]
  3. Ando Y., Tabei K., Asano Y. Luminal vasopressin modulates transport in the rabbit cortical collecting duct. J Clin Invest. 1991 Sep;88(3):952–959. doi: 10.1172/JCI115398. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Baddouri K., Butlen D., Imbert-Teboul M., Le Bouffant F., Marchetti J., Chabardès D., Morel F. Plasma antidiuretic hormone levels and kidney responsiveness to vasopressin in the jerboa, Jaculus orientalis. Gen Comp Endocrinol. 1984 May;54(2):203–215. doi: 10.1016/0016-6480(84)90174-6. [DOI] [PubMed] [Google Scholar]
  5. Birnbaumer M., Antaramian A., Themmen A. P., Gilbert S. Desensitization of the human V2 vasopressin receptor. Homologous effects in the absence of heterologous desensitization. J Biol Chem. 1992 Jun 15;267(17):11783–11788. [PubMed] [Google Scholar]
  6. Birnbaumer M., Seibold A., Gilbert S., Ishido M., Barberis C., Antaramian A., Brabet P., Rosenthal W. Molecular cloning of the receptor for human antidiuretic hormone. Nature. 1992 May 28;357(6376):333–335. doi: 10.1038/357333a0. [DOI] [PubMed] [Google Scholar]
  7. Burnatowska-Hledin M. A., Spielman W. S. Vasopressin V1 receptors on the principal cells of the rabbit cortical collecting tubule. Stimulation of cytosolic free calcium and inositol phosphate production via coupling to a pertussis toxin substrate. J Clin Invest. 1989 Jan;83(1):84–89. doi: 10.1172/JCI113888. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Catt K. J., Harwood J. P., Aguilera G., Dufau M. L. Hormonal regulation of peptide receptors and target cell responses. Nature. 1979 Jul 12;280(5718):109–116. doi: 10.1038/280109a0. [DOI] [PubMed] [Google Scholar]
  9. Chabardès D., Gagnan-Brunette M., Imbert-Teboul M., Gontcharevskaia O., Montégut M., Clique A., Morel F. Adenylate cyclase responsiveness to hormones in various portions of the human nephron. J Clin Invest. 1980 Feb;65(2):439–448. doi: 10.1172/JCI109687. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Collins S., Bouvier M., Bolanowski M. A., Caron M. G., Lefkowitz R. J. cAMP stimulates transcription of the beta 2-adrenergic receptor gene in response to short-term agonist exposure. Proc Natl Acad Sci U S A. 1989 Jul;86(13):4853–4857. doi: 10.1073/pnas.86.13.4853. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Dorsa D. M., Majumdar L. A., Petracca F. M., Baskin D. G., Cornett L. E. Characterization and localization of 3H-arginine8-vasopressin binding to rat kidney and brain tissue. Peptides. 1983 Sep-Oct;4(5):699–706. doi: 10.1016/0196-9781(83)90021-9. [DOI] [PubMed] [Google Scholar]
  12. Dworkin L. D., Ichikawa I., Brenner B. M. Hormonal modulation of glomerular function. Am J Physiol. 1983 Feb;244(2):F95–104. doi: 10.1152/ajprenal.1983.244.2.F95. [DOI] [PubMed] [Google Scholar]
  13. Elalouf J. M., Di Stefano A., de Rouffignac C. Sensitivities of rat kidney thick ascending limbs and collecting ducts to vasopressin in vivo. Proc Natl Acad Sci U S A. 1986 Apr;83(7):2276–2280. doi: 10.1073/pnas.83.7.2276. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Fort P., Marty L., Piechaczyk M., el Sabrouty S., Dani C., Jeanteur P., Blanchard J. M. Various rat adult tissues express only one major mRNA species from the glyceraldehyde-3-phosphate-dehydrogenase multigenic family. Nucleic Acids Res. 1985 Mar 11;13(5):1431–1442. doi: 10.1093/nar/13.5.1431. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Hebert S. C., Schafer J. A., Andreoli T. E. The effects of antidiuretic hormone (ADH) on solute and water transport in the mammalian nephron. J Membr Biol. 1981 Jan 30;58(1):1–19. doi: 10.1007/BF01871030. [DOI] [PubMed] [Google Scholar]
  16. Hirono A., Beutler E. Alternative splicing of human glucose-6-phosphate dehydrogenase messenger RNA in different tissues. J Clin Invest. 1989 Jan;83(1):343–346. doi: 10.1172/JCI113881. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Imbert M., Chabardès D., Montegut M., Clique A., Morel F. Vasopressin dependent adenylate cyclase in single segments of rabbit kidney tubule. Pflugers Arch. 1975 Jun 26;357(3-4):173–186. doi: 10.1007/BF00585973. [DOI] [PubMed] [Google Scholar]
  18. Ishikawa S., Okada K., Saito T. Arginine vasopressin increases cellular free calcium concentration and adenosine 3',5'-monophosphate production in rat renal papillary collecting tubule cells in culture. Endocrinology. 1988 Sep;123(3):1376–1384. doi: 10.1210/endo-123-3-1376. [DOI] [PubMed] [Google Scholar]
  19. Jaisser F., Bugeon L., Blot-Chabaud M., Bonvalet J. P., Farman N. Effects of AVP and dDAVP on PGE2 synthesis in superfused cortical collecting tubules. Am J Physiol. 1989 Jun;256(6 Pt 2):F1044–F1050. doi: 10.1152/ajprenal.1989.256.6.F1044. [DOI] [PubMed] [Google Scholar]
  20. Jard S., Lombard C., Marie J., Devilliers G. Vasopressin receptors from cultured mesangial cells resemble V1a type. Am J Physiol. 1987 Jul;253(1 Pt 2):F41–F49. doi: 10.1152/ajprenal.1987.253.1.F41. [DOI] [PubMed] [Google Scholar]
  21. Knepper M., Burg M. Organization of nephron function. Am J Physiol. 1983 Jun;244(6):F579–F589. doi: 10.1152/ajprenal.1983.244.6.F579. [DOI] [PubMed] [Google Scholar]
  22. Landgraf R., Szot P., Dorsa D. M. Vasopressin receptors in the brain, liver and kidney of rats following osmotic stimulation. Brain Res. 1991 Mar 29;544(2):287–290. doi: 10.1016/0006-8993(91)90066-5. [DOI] [PubMed] [Google Scholar]
  23. Lolait S. J., O'Carroll A. M., McBride O. W., Konig M., Morel A., Brownstein M. J. Cloning and characterization of a vasopressin V2 receptor and possible link to nephrogenic diabetes insipidus. Nature. 1992 May 28;357(6376):336–339. doi: 10.1038/357336a0. [DOI] [PubMed] [Google Scholar]
  24. Maeda Y., Han J. S., Gibson C. C., Knepper M. A. Vasopressin and oxytocin receptors coupled to Ca2+ mobilization in rat inner medullary collecting duct. Am J Physiol. 1993 Jul;265(1 Pt 2):F15–F25. doi: 10.1152/ajprenal.1993.265.1.F15. [DOI] [PubMed] [Google Scholar]
  25. Michell R. H., Kirk C. J., Billah M. M. Hormonal stimulation of phosphatidylinositol breakdown with particular reference to the hepatic effects of vasopressin. Biochem Soc Trans. 1979 Oct;7(5):861–865. doi: 10.1042/bst0070861. [DOI] [PubMed] [Google Scholar]
  26. Morel A., O'Carroll A. M., Brownstein M. J., Lolait S. J. Molecular cloning and expression of a rat V1a arginine vasopressin receptor. Nature. 1992 Apr 9;356(6369):523–526. doi: 10.1038/356523a0. [DOI] [PubMed] [Google Scholar]
  27. Morel F. Sites of hormone action in the mammalian nephron. Am J Physiol. 1981 Mar;240(3):F159–F164. doi: 10.1152/ajprenal.1981.240.3.F159. [DOI] [PubMed] [Google Scholar]
  28. Moriyama T., Murphy H. R., Martin B. M., Garcia-Perez A. Detection of specific mRNAs in single nephron segments by use of the polymerase chain reaction. Am J Physiol. 1990 May;258(5 Pt 2):F1470–F1474. doi: 10.1152/ajprenal.1990.258.5.F1470. [DOI] [PubMed] [Google Scholar]
  29. Nitschke R., Fröbe U., Greger R. Antidiuretic hormone acts via V1 receptors on intracellular calcium in the isolated perfused rabbit cortical thick ascending limb. Pflugers Arch. 1991 Feb;417(6):622–632. doi: 10.1007/BF00372961. [DOI] [PubMed] [Google Scholar]
  30. Nonoguchi H., Tomita K., Marumo F. Effects of atrial natriuretic peptide and vasopressin on chloride transport in long- and short-looped medullary thick ascending limbs. J Clin Invest. 1992 Aug;90(2):349–357. doi: 10.1172/JCI115869. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Ostrowski N. L., Lolait S. J., Bradley D. J., O'Carroll A. M., Brownstein M. J., Young W. S., 3rd Distribution of V1a and V2 vasopressin receptor messenger ribonucleic acids in rat liver, kidney, pituitary and brain. Endocrinology. 1992 Jul;131(1):533–535. doi: 10.1210/endo.131.1.1535312. [DOI] [PubMed] [Google Scholar]
  32. Phillips P. A., Abrahams J. M., Kelly J. M., Mooser V., Trinder D., Johnston C. I. Localization of vasopressin binding sites in rat tissues using specific V1 and V2 selective ligands. Endocrinology. 1990 Mar;126(3):1478–1484. doi: 10.1210/endo-126-3-1478. [DOI] [PubMed] [Google Scholar]
  33. Ruggles B. T., Murayama N., Werness J. L., Gapstur S. M., Bentley M. D., Dousa T. P. The vasopressin-sensitive adenylate cyclase in collecting tubules and in thick ascending limb of Henle's loop of human and canine kidney. J Clin Endocrinol Metab. 1985 May;60(5):914–921. doi: 10.1210/jcem-60-5-914. [DOI] [PubMed] [Google Scholar]
  34. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Schlondorff D., Yoo P., Alpert B. E. Stimulation of adenylate cyclase in isolated rat glomeruli by prostaglandins. Am J Physiol. 1978 Nov;235(5):F458–F464. doi: 10.1152/ajprenal.1978.235.5.F458. [DOI] [PubMed] [Google Scholar]
  36. Schor N., Ichikawa I., Brenner B. M. Mechanisms of action of various hormones and vasoactive substances on glomerular ultrafiltration in the rat. Kidney Int. 1981 Oct;20(4):442–451. doi: 10.1038/ki.1981.160. [DOI] [PubMed] [Google Scholar]
  37. Star R. A., Nonoguchi H., Balaban R., Knepper M. A. Calcium and cyclic adenosine monophosphate as second messengers for vasopressin in the rat inner medullary collecting duct. J Clin Invest. 1988 Jun;81(6):1879–1888. doi: 10.1172/JCI113534. [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. Steiner M., Phillips M. I. Renal tubular vasopressin receptors downregulated by dehydration. Am J Physiol. 1988 Mar;254(3 Pt 1):C404–C410. doi: 10.1152/ajpcell.1988.254.3.C404. [DOI] [PubMed] [Google Scholar]
  39. Teitelbaum I. Vasopressin-stimulated phosphoinositide hydrolysis in cultured rat inner medullary collecting duct cells is mediated by the oxytocin receptor. J Clin Invest. 1991 Jun;87(6):2122–2126. doi: 10.1172/JCI115243. [DOI] [PMC free article] [PubMed] [Google Scholar]
  40. Terada Y., Moriyama T., Martin B. M., Knepper M. A., Garcia-Perez A. RT-PCR microlocalization of mRNA for guanylyl cyclase-coupled ANF receptor in rat kidney. Am J Physiol. 1991 Dec;261(6 Pt 2):F1080–F1087. doi: 10.1152/ajprenal.1991.261.6.F1080. [DOI] [PubMed] [Google Scholar]
  41. Torikai S., Kurokawa K. Effect of PGE2 on vasopressin-dependent cell cAMP in isolated single nephron segments. Am J Physiol. 1983 Jul;245(1):F58–F66. doi: 10.1152/ajprenal.1983.245.1.F58. [DOI] [PubMed] [Google Scholar]
  42. Ujiie K., Nonoguchi H., Tomita K., Marumo F. Effects of ANF on cGMP synthesis in inner medullary collecting duct subsegments of rats. Am J Physiol. 1990 Sep;259(3 Pt 2):F535–F538. doi: 10.1152/ajprenal.1990.259.3.F535. [DOI] [PubMed] [Google Scholar]
  43. Wittner M., di Stefano A., Wangemann P., Nitschke R., Greger R., Bailly C., Amiel C., Roinel N., de Rouffignac C. Differential effects of ADH on sodium, chloride, potassium, calcium and magnesium transport in cortical and medullary thick ascending limbs of mouse nephron. Pflugers Arch. 1988 Oct;412(5):516–523. doi: 10.1007/BF00582541. [DOI] [PubMed] [Google Scholar]
  44. Wuthrich R. P., Vallotton M. B. Prostaglandin E2 and cyclic AMP response to vasopressin in renal medullary tubular cells. Am J Physiol. 1986 Sep;251(3 Pt 2):F499–F505. doi: 10.1152/ajprenal.1986.251.3.F499. [DOI] [PubMed] [Google Scholar]
  45. Ysewijn-Van Brussel K. A., De Leenheer A. P. Development and evaluation of a radioimmunoassay for Arg8-vasopressin, after extraction with Sep-Pak C18. Clin Chem. 1985 Jun;31(6):861–863. [PubMed] [Google Scholar]
  46. van Leeuwen F. W., van der Beek E. M., van Heerikhuize J. J., Wolters P., van der Meulen G., Wan Y. P. Quantitative light microscopic autoradiographic localization of binding sites labelled with [3H]vasopressin antagonist d(CH2)5Tyr(Me)VP in the rat brain, pituitary and kidney. Neurosci Lett. 1987 Sep 23;80(2):121–126. doi: 10.1016/0304-3940(87)90640-9. [DOI] [PubMed] [Google Scholar]

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