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. 1980 Jan;33(1):69–77. doi: 10.1128/jvi.33.1.69-77.1980

Comparisons of nucleotide sequences in the genomes of the New Jersey and Indiana serotypes of vesicular stomatitis virus.

D J McGeoch, A Dolan, C R Pringle
PMCID: PMC288524  PMID: 6245255

Abstract

Nucleotide sequences of around 200 residues were determined adjacent to the 3' terminus of the genome RNA of vesicular stomatitis virus, New Jersey serotype, and adjacent to the 3'-terminal polyadenylic acid tract of the N protein mRNA of the same virus. These sequences were compared with the corresponding sequences previously determined for the Indiana serotype of vesicular stomatitis virus. The sequences obtained for the two strains were readily aligned, showing 70.8% homology overall. Examination of the sequences allowed identification of the translation initiation and termination codons for the N mRNA of each serotype. The deduced N-terminal and C-terminal amino acid sequences of the two N polypeptides were each similar, and most of the differences between them consisted of substitution by a clearly homologous amino acid. It was proposed that these nucleotide sequences, within limits imposed by their functions, comprise reasonably representative measures of the extent of sequence homology between the genomes of the two serotypes, and that this is higher than previously estimated, but with little exact homology over extended regions.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Banerjee A. D., Abraham G., Colonno R. J. Vesicular stomatitis virus: mode of transcription. J Gen Virol. 1977 Jan;34(1):1–8. doi: 10.1099/0022-1317-34-1-1. [DOI] [PubMed] [Google Scholar]
  2. Cartwright B., Brown F. Serological relationships between different strains of vesicular stomatis virus. J Gen Virol. 1972 Sep;16(3):391–398. doi: 10.1099/0022-1317-16-3-391. [DOI] [PubMed] [Google Scholar]
  3. Clewley J. P., Bishop D. H., Kang C. Y., Coffin J., Schnitzlein W. M., Reichmann M. E., Shope R. E. Oligonucleotide fingerprints of RNA species obtained from rhabdoviruses belonging to the vesicular stomatitis virus subgroup. J Virol. 1977 Jul;23(1):152–166. doi: 10.1128/jvi.23.1.152-166.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Colonno R. J., Banerjee A. K. Complete nucleotide sequence of the leader RNA synthesized in vitro by vesicular stomatitis virus. Cell. 1978 Sep;15(1):93–101. doi: 10.1016/0092-8674(78)90085-5. [DOI] [PubMed] [Google Scholar]
  5. Colonno R. J., Banerjee A. K. Nucleotide sequence of the leader RNA of the New Jersey serotype of vesicular stomatitis virus. Nucleic Acids Res. 1978 Nov;5(11):4165–4176. doi: 10.1093/nar/5.11.4165. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Doel T. R., Brown F. Tryptic peptide analysis of the structural proteins of vesicular stomatitis virus. J Gen Virol. 1978 Feb;38(2):351–361. doi: 10.1099/0022-1317-38-2-351. [DOI] [PubMed] [Google Scholar]
  7. Franze-Fernandez M. T., Banerjee A. K. In vitro RNA transcription by the New Jersey serotype of vesicular stomatitis virus. I. Characterization of the mRNA species. J Virol. 1978 Apr;26(1):179–187. doi: 10.1128/jvi.26.1.179-187.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Freeman G. J., Rao D. D., Huang A. S. RNA synthesis of vesicular stomatitis virus. VIII. Oligonucleotides of the structural genes and mRNA. Gene. 1979 Feb;5(2):141–157. doi: 10.1016/0378-1119(79)90099-4. [DOI] [PubMed] [Google Scholar]
  9. Kang C. Y., Prevec L. Proteins of vesicular stomatitis virus. II. Immunological comparisons of viral antigens. J Virol. 1970 Jul;6(1):20–27. doi: 10.1128/jvi.6.1.20-27.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Keene J. D., Schubert M., Lazzarini R. A., Rosenberg M. Nucleotide sequence homology at the 3' termini of RNA from vesicular stomatitis virus and its defective interfering particles. Proc Natl Acad Sci U S A. 1978 Jul;75(7):3225–3229. doi: 10.1073/pnas.75.7.3225. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Maxam A. M., Gilbert W. A new method for sequencing DNA. Proc Natl Acad Sci U S A. 1977 Feb;74(2):560–564. doi: 10.1073/pnas.74.2.560. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. McGeoch D. J., Dolan A. Sequence of 200 nucleotides at the 3'-terminus of the genome RNA of vesicular stomatitis virus. Nucleic Acids Res. 1979 Jul 25;6(10):3199–3211. doi: 10.1093/nar/6.10.3199. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. McGeoch D. J. Structure of the gene N:gene NS intercistronic junction in the genome of vesicular stomatitis virus. Cell. 1979 Jul;17(3):673–681. doi: 10.1016/0092-8674(79)90274-5. [DOI] [PubMed] [Google Scholar]
  14. McGeoch D. J., Turnbull N. T. Analysis of the 3'-terminal nucleotide sequence of vesicular stomatitis virus N protein mRNA. Nucleic Acids Res. 1978 Nov;5(11):4007–4024. doi: 10.1093/nar/5.11.4007. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Pringle C. R., Duncan I. B., Stevenson M. Isolation and characterization of temperature-sensitive mutants of vesicular stomatitis virus, New Jersey serotype. J Virol. 1971 Dec;8(6):836–841. doi: 10.1128/jvi.8.6.836-841.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Reichmann M. E., Schnitzlein W. M., Bishop D. H., Lazzerini R. A., Beatrice S. T., Wagner R. R. Classification of the New Jersey serotype of vesicular stomatitis virus into two subtypes. J Virol. 1978 Jan;25(1):446–449. doi: 10.1128/jvi.25.1.446-449.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Repik P., Flamand A., Clark H. F., Obijeski J. F., Roy P., Bishop D. H. Detection of homologous RNA sequences among six rhabdovirus genomes. J Virol. 1974 Jan;13(1):250–252. doi: 10.1128/jvi.13.1.250-252.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Rose J. K. Complete sequences of the ribosome recognition sites in vesicular stomatitis virus mRNAs: recognition by the 40S and 80S complexes. Cell. 1978 Jun;14(2):345–353. doi: 10.1016/0092-8674(78)90120-4. [DOI] [PubMed] [Google Scholar]
  19. Sadgopal A. The genetic code after the excitement. Adv Genet. 1968;14:325–404. [PubMed] [Google Scholar]

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