Abstract
We have established a persistent infection of BHK cells with a preparation of Sindbis virus heavily enriched in defective interfering (DI) particles. The small fraction of cells that survived the initial infection grew out to form a stable population of cells [BHK(Sin-1) cells], most of which synthesized viral RNA and viral antigens. The presence of DI particles in this virus stock was required to establish this persistent state. BHK(Sin-1) cells released a small-plaque, temperature-sensitive virus (Sin-1 virus) as well as DI particles containing DI RNAs larger than those present in the original stock used to establish the persistent state. A cloned stock of Sin-1 virus, free of detectable DI particles, was able to initiate a persistent infection more quickly and with greater cell survival than the original stock of Sindbis virus containing DI particles. About 2 weeks after the Sin-1 virus-infected cells were cultured, DI RNAs arose and soon became the dominant viral RNA species produced by these cells.
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Selected References
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- CHAMBERS V. C. The prolonged persistence of western equine encephalomyelitis virus in cultures of strain L cells. Virology. 1957 Feb;3(1):62–75. doi: 10.1016/0042-6822(57)90023-5. [DOI] [PubMed] [Google Scholar]
- Eaton B. T. Evidence for the synthesis of defection interfering particles by Aedes albopictus cells persistently infected with Sindbis virus. Virology. 1977 Apr;77(2):843–848. doi: 10.1016/0042-6822(77)90503-7. [DOI] [PubMed] [Google Scholar]
- Fan D. P., Sefton B. M. The entry into host cells of Sindbis virus, vesicular stomatitis virus and Sendai virus. Cell. 1978 Nov;15(3):985–992. doi: 10.1016/0092-8674(78)90282-9. [DOI] [PubMed] [Google Scholar]
- Holland J. J., Grabau E. A., Jones C. L., Semler B. L. Evolution of multiple genome mutations during long-term persistent infection by vesicular stomatitis virus. Cell. 1979 Mar;16(3):495–504. doi: 10.1016/0092-8674(79)90024-2. [DOI] [PubMed] [Google Scholar]
- Holland J. J., Villarreal L. P., Welsh R. M., Oldstone M. B., Kohne D., Lazzarini R., Scolnick E. Long-term persistent vesicular stomatitis virus and rabies virus infection of cells in vitro. J Gen Virol. 1976 Nov;33(2):193–211. doi: 10.1099/0022-1317-33-2-193. [DOI] [PubMed] [Google Scholar]
- Huang A. S., Baltimore D. Defective viral particles and viral disease processes. Nature. 1970 Apr 25;226(5243):325–327. doi: 10.1038/226325a0. [DOI] [PubMed] [Google Scholar]
- Inglot A. D., Albin M., Chudzio T. Persistent infection of mouse cells with Sindbis virus: role of virulence of strains, auto-interfering particles and interferon. J Gen Virol. 1973 Jul;20(1):105–110. doi: 10.1099/0022-1317-20-1-105. [DOI] [PubMed] [Google Scholar]
- Jacobson S., Dutko F. J., Pfau C. J. Determinants of spontaneous recovery and persistance in MDCK cells infected with lymphocytic choriomeningitis virus. J Gen Virol. 1979 Jul;44(1):113–122. doi: 10.1099/0022-1317-44-1-113. [DOI] [PubMed] [Google Scholar]
- Kennedy S. I., Bruton C. J., Weiss B., Schlesinger S. Defective interfering passages of Sindbis virus: nature of the defective virion RNA. J Virol. 1976 Sep;19(3):1034–1043. doi: 10.1128/jvi.19.3.1034-1043.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Logan K. B. Generation of defective interfering particles of Semliki Forest virus in a clone of Aedes albopictus (mosquito) cells. J Virol. 1979 Apr;30(1):38–44. doi: 10.1128/jvi.30.1.38-44.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McMaster G. K., Carmichael G. G. Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange. Proc Natl Acad Sci U S A. 1977 Nov;74(11):4835–4838. doi: 10.1073/pnas.74.11.4835. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nishiyama Y., Ito Y., Shimokata K. Properties of the viruses selected during persistent infection of L cells with VSV. J Gen Virol. 1978 Aug;40(2):481–484. doi: 10.1099/0022-1317-40-2-481. [DOI] [PubMed] [Google Scholar]
- Nishiyama Y. Studies of L cells persistently infected with VSV: factors involved in the regulation of persistent infection. J Gen Virol. 1977 May;35(2):265–279. doi: 10.1099/0022-1317-35-2-265. [DOI] [PubMed] [Google Scholar]
- Preble O. T., Youngner J. S. Temperature-sensitive viruses and the etiology of chronic and inapparent infections. J Infect Dis. 1975 Apr;131(4):467–473. doi: 10.1093/infdis/131.4.467. [DOI] [PubMed] [Google Scholar]
- Ramseur J. M., Friedman R. M. Prolonged infection of L cells with vesicular stomatitis virus. Defective interfering forms and temperature-sensitive mutants as factors in the infection. Virology. 1978 Mar;85(1):253–261. doi: 10.1016/0042-6822(78)90429-4. [DOI] [PubMed] [Google Scholar]
- Riedel B., Brown D. T. Novel antiviral activity found in the media of Sindbis virus-persistently infected mosquito (Aedes albopictus) cell cultures. J Virol. 1979 Jan;29(1):51–60. doi: 10.1128/jvi.29.1.51-60.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Roux L., Holland J. J. Role of defective interfering particles of Sendai virus in persistent infections. Virology. 1979 Feb;93(1):91–103. doi: 10.1016/0042-6822(79)90278-2. [DOI] [PubMed] [Google Scholar]
- Schwöbel W., Ahl R. Peristence of sindbis virus in BHK-21 cell cultures. Arch Gesamte Virusforsch. 1972;38(1):1–10. doi: 10.1007/BF01241350. [DOI] [PubMed] [Google Scholar]
- Shenk T. E., Koshelnyk K. A., Stollar V. Temperature-sensitive virus from Aedes albopictus cells chronically infected with Sindbis virus. J Virol. 1974 Feb;13(2):439–447. doi: 10.1128/jvi.13.2.439-447.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Spandidos D. A., Graham A. F. Generation of defective virus after infection of newborn rats with reovirus. J Virol. 1976 Oct;20(1):234–247. doi: 10.1128/jvi.20.1.234-247.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WALKER D. L. THE VIRAL CARRIER STATE IN ANIMAL CELL CULTURES. Prog Med Virol. 1964;6:111–148. [PubMed] [Google Scholar]
- Weiss B., Goran D., Cancedda R., Schlesinger S. Defective interfering passages of Sindbis virus: nature of the intracellular defective viral RNA. J Virol. 1974 Nov;14(5):1189–1198. doi: 10.1128/jvi.14.5.1189-1198.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Weiss B., Schlesinger S. Defective interfering passages of Sindbis virus: chemical composition, biological activity, and mode of interference. J Virol. 1973 Oct;12(4):862–871. doi: 10.1128/jvi.12.4.862-871.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Welsh R. M., Oldstone M. B. Inhibition of immunologic injury of cultured cells infected with lymphocytic choriomeningitis virus: role of defective interfering virus in regulating viral antigenic expression. J Exp Med. 1977 Jun 1;145(6):1449–1468. doi: 10.1084/jem.145.6.1449. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Youngner J. S., Dubovi E. J., Quagliana D. O., Kelly M., Preble O. T. Role of temperature-sensitive mutants in persistent infections initiated with vesicular stomatitis virus. J Virol. 1976 Jul;19(1):90–101. doi: 10.1128/jvi.19.1.90-101.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Youngner J. S., Preble O. T., Jones E. V. Persistent infection of L cells with vesicular stomatitis virus: evolution of virus populations. J Virol. 1978 Oct;28(1):6–12. doi: 10.1128/jvi.28.1.6-13.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Youngner J. S., Quagliana D. O. Temperature-sensitive mutants isolated from hamster and canine cell lines persistently infected with Newcastle disease virus. J Virol. 1975 Nov;16(5):1332–1336. doi: 10.1128/jvi.16.5.1332-1336.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]