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. 1980 Jan;33(1):535–538. doi: 10.1128/jvi.33.1.535-538.1980

Early events after infection of Escherichia coli by bacteriophage T5. III. Inhibition of uracil-DNA glycosylase activity.

H R Warner, L K Johnson, D P Snustad
PMCID: PMC288568  PMID: 6245250

Abstract

The activity of uracil-DNA glycosylase in Escherichia coli decreases dramatically to less than 10% of its original level after infection of the cells by phage T5. Phage-induced protein synthesis is required for this inhibition to occur, and the inhibition is induced by a mutant capable of injecting only the first 8% of its DNA. The inhibitor activity in extracts of infected cells is heat labile and nondialyzable, and will inhibit enzyme activity present in extracts of uninfected cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Berget S. M., Mozer T. J., Warner H. R. Early events after infection of Escherichia coli by bacteriophage T5. II. Control of the bacteriophage-induced 5'-nucleotidase activity. J Virol. 1976 Apr;18(1):71–79. doi: 10.1128/jvi.18.1.71-79.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Berget S. M., Warner H. R., McCorquodale D. J. Isolation and partial characterization of bacteriophage T5 mutants deficient in the ability to induce deoxynucleoside monophosphate kinase. J Virol. 1974 Jul;14(1):78–85. doi: 10.1128/jvi.14.1.78-85.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bujard H. Location of single-strand interruptions in the DNA of bacteriophage T5. Proc Natl Acad Sci U S A. 1969 Apr;62(4):1167–1174. doi: 10.1073/pnas.62.4.1167. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Duncan B. K., Rockstroh P. A., Warner H. R. Escherichia coli K-12 mutants deficient in uracil-DNA glycosylase. J Bacteriol. 1978 Jun;134(3):1039–1045. doi: 10.1128/jb.134.3.1039-1045.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Duncan B. K., Warner H. R. Metabolism of uracil-containing DNA: degradation of bacteriophage PBS2 DNA in Bacillus subtilis. J Virol. 1977 Jun;22(3):835–838. doi: 10.1128/jvi.22.3.835-838.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Duncan J., Hamilton L., Friedberg E. C. Enzymatic degradation of uracil-containing DNA. II. Evidence for N-glycosidase and nuclease activities in unfractionated extracts of Bacillus subtilis. J Virol. 1976 Aug;19(2):338–345. doi: 10.1128/jvi.19.2.338-345.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Friedberg E. C., Ganesan A. K., Minton K. N-Glycosidase activity in extracts of Bacillus subtilis and its inhibition after infection with bacteriophage PBS2. J Virol. 1975 Aug;16(2):315–321. doi: 10.1128/jvi.16.2.315-321.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hayward G. S., Smith M. G. The chromosome of bacteriophage T5. I. Analysis of the single-stranded DNA fragments by agarose gel electrophoresis. J Mol Biol. 1972 Feb 14;63(3):383–395. doi: 10.1016/0022-2836(72)90435-4. [DOI] [PubMed] [Google Scholar]
  9. Hendrickson H. E., McCorquodale D. J. Genetic and physiological studies of bacteriophage t5 I. An expanded genetic map of t5. J Virol. 1971 May;7(5):612–618. doi: 10.1128/jvi.7.5.612-618.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hochhauser S. J., Weiss B. Escherichia coli mutants deficient in deoxyuridine triphosphatase. J Bacteriol. 1978 Apr;134(1):157–166. doi: 10.1128/jb.134.1.157-166.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Katz G. E., Price A. R., Pomerantz M. J. Bacteriophage PBS2-induced inhibition of uracil-containing DNA degradation. J Virol. 1976 Nov;20(2):535–538. doi: 10.1128/jvi.20.2.535-538.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  13. Lanni Y. Functions of two genes in the first-step-transfer DNA of bacteriophage T5. J Mol Biol. 1969 Aug 28;44(1):173–183. doi: 10.1016/0022-2836(69)90412-4. [DOI] [PubMed] [Google Scholar]
  14. Lindahl T., Ljungquist S., Siegert W., Nyberg B., Sperens B. DNA N-glycosidases: properties of uracil-DNA glycosidase from Escherichia coli. J Biol Chem. 1977 May 25;252(10):3286–3294. [PubMed] [Google Scholar]
  15. McCorquodale D. J., Shaw A. R., Shaw P. K., Chinnadurai G. Pre-early polypeptides of bacteriophages T5 and BF23. J Virol. 1977 May;22(2):480–488. doi: 10.1128/jvi.22.2.480-488.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Rogers S. G., Godwin E. A., Shinosky E. S., Rhoades M. Interruption-deficient mutants of bacteriophage T5 I. Isolation and general properties. J Virol. 1979 Feb;29(2):716–725. doi: 10.1128/jvi.29.2.716-725.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Shlomai J., Kornberg A. Deoxyuridine triphosphatase of Escherichia coli. Purification, properties, and use as a reagent to reduce uracil incorporation into DNA. J Biol Chem. 1978 May 10;253(9):3305–3312. [PubMed] [Google Scholar]
  18. Warner H. R., Drong R. F., Berget S. M. Early events after infection of Escherichia coli by bacteriophage T5. Induction of a 5'-nucleotidase activity and excretion of free bases. J Virol. 1975 Feb;15(2):273–280. doi: 10.1128/jvi.15.2.273-280.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Warner H. R., Thompson R. B., Mozer T. J., Duncan B. K. The properties of a bacteriophage T5 mutant unable to induce deoxyuridine 5'-triphosphate nucleotidohydrolase. Synthesis of uracil-containing T5 deoxyribonucleic acid. J Biol Chem. 1979 Aug 25;254(16):7534–7539. [PubMed] [Google Scholar]

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