Abstract
The bacteriophage T4-induced alt and mod gene products covalently add ADP-ribose to the Escherichia coli RNA polymerase alpha polypeptides; phage carrying either an alt or a mod mutation are viable. A genetic cross between T4alt and T4mod phages yielded alt mod recombinant progeny which could not ADP ribosylate RNA polymerase at all, yet grew apparently normally. Thus, ADP ribosylation of RNA polymerase appeared to be nonessential for T4 development (at least in E. coli B/r and E. coli CR63), even though the phage has evolved two distinct enzymes to catalyze this reaction.
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- Goff C. G. Bacteriophage T4 alt gene maps between genes 30 and 54. J Virol. 1979 Mar;29(3):1232–1234. doi: 10.1128/jvi.29.3.1232-1234.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Goff C. G. Chemical structure of a modification of the Escherichia coli ribonucleic acid polymerase alpha polypeptides induced by bacteriophage T4 infection. J Biol Chem. 1974 Oct 10;249(19):6181–6190. [PubMed] [Google Scholar]
- Horvitz H. R. Bacteriophage T4 mutants deficient in alteration and modification of the Escherichia coli RNA polymerase. J Mol Biol. 1974 Dec 25;90(4):739–750. doi: 10.1016/0022-2836(74)90537-3. [DOI] [PubMed] [Google Scholar]
- Horvitz H. R. Control by bacteriophage T4 of two sequential phosphorylations of the alpha subunit of Escherichia coli RNA polymerase. J Mol Biol. 1974 Dec 25;90(4):727–738. doi: 10.1016/0022-2836(74)90536-1. [DOI] [PubMed] [Google Scholar]
- Rohrer H., Zillig W., Mailhammer R. ADP-ribosylation of DNA-dependent RNA polymerase of Escherichia coli by an NAD+: protein ADP-ribosyltransferase from bacteriophage T4. Eur J Biochem. 1975 Dec 1;60(1):227–238. doi: 10.1111/j.1432-1033.1975.tb20995.x. [DOI] [PubMed] [Google Scholar]
- Skórko R., Zillig W., Rohrer H., Fujiki H., Mailhammer R. Purification and properties of the NAD+: protein ADP-ribosyltransferase responsible for the T4-phage-induced modification of the alpha subunit of DNA-dependent RNA polymerase of Escherichia coli. Eur J Biochem. 1977 Sep 15;79(1):55–66. doi: 10.1111/j.1432-1033.1977.tb11783.x. [DOI] [PubMed] [Google Scholar]
- Walter G., Seifert W., Zillig W. Modified DNA-dependent RNA polymerase from E. coli infected with bacteriophage T4. Biochem Biophys Res Commun. 1968 Feb 15;30(3):240–247. doi: 10.1016/0006-291x(68)90441-5. [DOI] [PubMed] [Google Scholar]
- Wilson J. H. Function of the bacteriophage T4 transfer RNA's. J Mol Biol. 1973 Mar 15;74(4):753–757. doi: 10.1016/0022-2836(73)90065-x. [DOI] [PubMed] [Google Scholar]