Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1980 Jan;33(1):553–556. doi: 10.1128/jvi.33.1.553-556.1980

Fluorescence changes of a membrane-bound dye during bacteriophage T5 infection of Escherichia coli.

J Glenn, D H Duckworth
PMCID: PMC288573  PMID: 6988607

Abstract

The fluorescence intensity of membrane-bound N-phenyl-1-naphthylamine increases dramatically when T5 bacteriophage infect colicin Ib plasmid-containing hosts. This dramatic increase is not seen during normal infections or in infections wherein either the plasmid or the phage contain mutations which allow productive infection to occur. Two smaller increases in fluorescence intensity are seen, however, in all T5 infections in which the characteristic two-step injection of DNA can proceed.

Full text

PDF
553

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beckman L. D., Anderson G. C., McCorquodale D. J. Arrangement on the chromosome of the known pre-early genes of bacteriophages T5 and BF23. J Virol. 1973 Nov;12(5):1191–1194. doi: 10.1128/jvi.12.5.1191-1194.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Berger E. A. Different mechanisms of energy coupling for the active transport of proline and glutamine in Escherichia coli. Proc Natl Acad Sci U S A. 1973 May;70(5):1514–1518. doi: 10.1073/pnas.70.5.1514. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Berger E. A., Heppel L. A. Different mechanisms of energy coupling for the shock-sensitive and shock-resistant amino acid permeases of Escherichia coli. J Biol Chem. 1974 Dec 25;249(24):7747–7755. [PubMed] [Google Scholar]
  4. Brewer G. J. The state of energization of the membrane of Escherichia coli as affected by physiological conditions and colicin K. Biochemistry. 1976 Apr 6;15(7):1387–1392. doi: 10.1021/bi00652a006. [DOI] [PubMed] [Google Scholar]
  5. Cheung A. K., Duckworth D. H. Membrane damage in abortive infections of colicin Ib-containing Escherichia coli by bacteriophage T5. J Virol. 1977 Jul;23(1):98–105. doi: 10.1128/jvi.23.1.98-105.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Condit R. C. F factor-mediated inhibition of bacteriophage T7 growth: increased membrane permeability and decreased ATP levels following T7 infection of male Escherichia coli. J Mol Biol. 1975 Oct 15;98(1):45–59. doi: 10.1016/s0022-2836(75)80100-8. [DOI] [PubMed] [Google Scholar]
  7. Cramer W. A., Postma P. W., Helgerson S. L. An evaluation of N-phenyl-1-naphthylamine as a probe of membrane energy state in Escherichia coli. Biochim Biophys Acta. 1976 Dec 6;449(3):401–411. doi: 10.1016/0005-2728(76)90151-1. [DOI] [PubMed] [Google Scholar]
  8. Fields K. L. Comparison of the action of colicins E1 and K on Escherichia coli with the effects of abortive infection by virulent bacteriophages. J Bacteriol. 1969 Jan;97(1):78–82. doi: 10.1128/jb.97.1.78-82.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Fields K. L., Luria S. E. Effects of colicins E1 and K on transport systems. J Bacteriol. 1969 Jan;97(1):57–63. doi: 10.1128/jb.97.1.57-63.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. GAREN A. Physiological effects of rII mutations in bacteriophage T4. Virology. 1961 Jun;14:151–163. doi: 10.1016/0042-6822(61)90190-8. [DOI] [PubMed] [Google Scholar]
  11. Glenn J., Cheung A. K., Duckworth D. H. Are class I (pre-early) proteins of bacteriophage T5 sufficient to induce abortive infection of ColIb+ Escherichia coli? J Virol. 1979 May;30(2):431–437. doi: 10.1128/jvi.30.2.431-437.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Glenn J., Duckworth D. H. Amino acid and sugar transport in Escherichia coli (ColIb) during abortive infection by bacteriophage T5. J Virol. 1979 May;30(2):421–430. doi: 10.1128/jvi.30.2.421-430.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. HOFFEE P., ENGLESBERG E., LAMY F. THE GLUCOSE PERMEASE SYSTEM IN BACTERIA. Biochim Biophys Acta. 1964 Mar 30;79:337–350. [PubMed] [Google Scholar]
  14. Hantke K., Braun V. Fluorescence studies on first steps of phage-host interactions. Virology. 1974 Mar;58(1):310–312. doi: 10.1016/0042-6822(74)90167-6. [DOI] [PubMed] [Google Scholar]
  15. Helgerson S. L., Cramer W. A. Changes in E. coli cell envelope structure caused by uncouplers of active transport and colicin E1. J Supramol Struct. 1976;5(3):291–308. doi: 10.1002/jss.400050304. [DOI] [PubMed] [Google Scholar]
  16. Hendrickson H. E., McCorquodale D. J. Genetic and physiological studies of bacteriophage t5 I. An expanded genetic map of t5. J Virol. 1971 May;7(5):612–618. doi: 10.1128/jvi.7.5.612-618.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. KUNDIG W., GHOSH S., ROSEMAN S. PHOSPHATE BOUND TO HISTIDINE IN A PROTEIN AS AN INTERMEDIATE IN A NOVEL PHOSPHO-TRANSFERASE SYSTEM. Proc Natl Acad Sci U S A. 1964 Oct;52:1067–1074. doi: 10.1073/pnas.52.4.1067. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. LURIA S. E. ON THE MECHANISMS OF ACTION OF COLICINS. Ann Inst Pasteur (Paris) 1964 Nov;107:SUPPL–SUPPL:73. [PubMed] [Google Scholar]
  19. Lanni Y. T. First-step-transfer deoxyribonucleic acid of bacteriophage T5. Bacteriol Rev. 1968 Sep;32(3):227–242. doi: 10.1128/br.32.3.227-242.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Luria S. E. Colicins and the energetics of cell membranes. Sci Am. 1975 Dec;233(6):30–37. doi: 10.1038/scientificamerican1275-30. [DOI] [PubMed] [Google Scholar]
  21. McCorquodale D. J., Shaw A. R., Moody E. E., Hull R. A., Morgan A. F. Is abortive infection by bacteriophage BF23 of Escherichia coli harboring ColIb plasmids due to cell killing by internally liberated colicin Ib? J Virol. 1979 Jul;31(1):31–41. doi: 10.1128/jvi.31.1.31-41.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Moyer R. W., Fu A. S., Szabo C. Regulation of bacteriophage T5 development by ColI factors. J Virol. 1972 May;9(5):804–812. doi: 10.1128/jvi.9.5.804-812.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Nieva-Gomez D., Konisky J. Membrane changes in Escherichia coli induced by colicin Ia and agents known to disrupt energy transduction. Biochemistry. 1976 Jun 29;15(13):2747–2753. doi: 10.1021/bi00658a006. [DOI] [PubMed] [Google Scholar]
  24. Nisioka T., Ozeki H. Early abortive lysis by phage BF23 in Escherichia coli K-12 carrying the colicin Ib factor. J Virol. 1968 Nov;2(11):1249–1254. doi: 10.1128/jvi.2.11.1249-1254.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Oldmixon E., Braun V. Changes in fluorescence of 8-anilino-1-naphthalene sulfonate after bacteriophage T5 infection of Escherichia coli. Initial fluorescence rise coincides with onset of rubidium efflux. Biochim Biophys Acta. 1978 Jan 4;506(1):111–118. doi: 10.1016/0005-2736(78)90438-8. [DOI] [PubMed] [Google Scholar]
  26. Phillips S. K., Cramer W. A. Properties of the fluorescence probe response associated with the transmission mechanism of colicin E1. Biochemistry. 1973 Mar 13;12(6):1170–1176. doi: 10.1021/bi00730a024. [DOI] [PubMed] [Google Scholar]
  27. Plate C. A., Suit J. L., Jetten A. M., Luria S. E. Effects of colicin K on a mutant of Escherichia coli deficient in Ca 2+, Mg 2+-activated adenosine triphosphatase. J Biol Chem. 1974 Oct 10;249(19):6138–6143. [PubMed] [Google Scholar]
  28. Schein S. J., Kagan B. L., Finkelstein A. Colicin K acts by forming voltage-dependent channels in phospholipid bilayer membranes. Nature. 1978 Nov 9;276(5684):159–163. doi: 10.1038/276159a0. [DOI] [PubMed] [Google Scholar]
  29. Strobel M., Nomura M. Restriction of the growth of bacteriophage BF23 by a colicine I (Col I-P9) factor. Virology. 1966 Apr;28(4):763–765. doi: 10.1016/0042-6822(66)90263-7. [DOI] [PubMed] [Google Scholar]
  30. Susskind M. M., Botstein D. Molecular genetics of bacteriophage P22. Microbiol Rev. 1978 Jun;42(2):385–413. doi: 10.1128/mr.42.2.385-413.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES