Skip to main content
Journal of Virology logoLink to Journal of Virology
. 1980 Jan;33(1):557–560. doi: 10.1128/jvi.33.1.557-560.1980

Gene K of bacteriophage phi X 174 codes for a nonessential protein.

E S Tessman, I Tessman, T J Pollock
PMCID: PMC288574  PMID: 6445011

Abstract

Gene K of phi X 174, which overlaps genes A, B, and C, was found to be nonessential, although possibly beneficial for the growth of the phage. Viable mutants of gene K made less than 4% of the normal amount of K protein as judged by quantitative fluorography of sodium dodecyl sulfate-polyacrylamide gels; compared with the wild-type phi X, K mutants had an identical latent period but a two- to threefold reduction in burst size.

Full text

PDF
557

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Burgess A. B. Studies on the proteins of phi X174. II. The protein composition of the phi X coat. Proc Natl Acad Sci U S A. 1969 Oct;64(2):613–617. doi: 10.1073/pnas.64.2.613. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Godson G. N., Barrell B. G., Staden R., Fiddes J. C. Nucleotide sequence of bacteriophage G4 DNA. Nature. 1978 Nov 16;276(5685):236–247. doi: 10.1038/276236a0. [DOI] [PubMed] [Google Scholar]
  3. Hutchison C. A., 3rd, Sinsheimer R. L. The process of infection with bacteriophage phi-X174. X. Mutations in a phi-X Lysis gene. J Mol Biol. 1966 Jul;18(3):429–447. doi: 10.1016/s0022-2836(66)80035-9. [DOI] [PubMed] [Google Scholar]
  4. Pollock T. J. Gene D of bacteriophage phi X 174: absence of transcriptional and translational regulatory properties. J Virol. 1977 Feb;21(2):468–474. doi: 10.1128/jvi.21.2.468-474.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Pollock T. J., Tessman E. S., Tessman I. Identification of lysis protein E of bacteriophage phiX174. J Virol. 1978 Oct;28(1):408–410. doi: 10.1128/jvi.28.1.408-410.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Pollock T. J., Tessman I., Tessman E. S. Potential for variability through multiple gene products of bacteriophage phiX174. Nature. 1978 Jul 6;274(5666):34–37. doi: 10.1038/274034a0. [DOI] [PubMed] [Google Scholar]
  7. Pollock T. J., Tessman I. Units of functional stability in bacteriophage messenger RNA. J Mol Biol. 1976 Dec 25;108(4):651–664. doi: 10.1016/s0022-2836(76)80110-6. [DOI] [PubMed] [Google Scholar]
  8. Sanger F., Air G. M., Barrell B. G., Brown N. L., Coulson A. R., Fiddes C. A., Hutchison C. A., Slocombe P. M., Smith M. Nucleotide sequence of bacteriophage phi X174 DNA. Nature. 1977 Feb 24;265(5596):687–695. doi: 10.1038/265687a0. [DOI] [PubMed] [Google Scholar]
  9. Shaw D. C., Walker J. E., Northrop F. D., Barrell B. G., Godson G. N., Fiddes J. C. Gene K, a new overlapping gene in bacteriophage G4. Nature. 1978 Apr 6;272(5653):510–515. doi: 10.1038/272510a0. [DOI] [PubMed] [Google Scholar]
  10. Smith M., Brown N. L., Air G. M., Barrell B. G., Coulson A. R., Hutchison C. A., 3rd, Sanger F. DNA sequence at the C termini of the overlapping genes A and B in bacteriophage phi X174. Nature. 1977 Feb 24;265(5596):702–705. doi: 10.1038/265702a0. [DOI] [PubMed] [Google Scholar]
  11. Weisbeek P. J., Borrias W. E., Langeveld S. A., Baas P. D., Van Arkel G. A. Bacteriophage phiX174: gene A overlaps gene B. Proc Natl Acad Sci U S A. 1977 Jun;74(6):2504–2508. doi: 10.1073/pnas.74.6.2504. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES