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. 1980 Jan;33(1):567–571. doi: 10.1128/jvi.33.1.567-571.1980

Characterization of RNA synthesized in isolated nuclei of herpes simplex virus type 1-infected KB cells.

M L Miovic, L I Pizer
PMCID: PMC288576  PMID: 6245253

Abstract

Nuclei isolated from herpes simplex virus (HSV)-infected KB cells actively synthesize HSV RNA in vitro; the RNA can be hybridized with HSV DNA or nuclear RNA from HSV-infected cells. Nascent RNA molecules labeled in vivo with 32PO4 were elongated, utilizing the nuclear system to incorporate Hg-CTP at their 3' ends, and then isolated on an affinity column. Hybridization of isolated nascent RNA molecules showed that greater than 50% of them were HSV specific and that more than 25% were self-complementary.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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