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. 1980 Feb;33(2):769–773. doi: 10.1128/jvi.33.2.769-773.1980

Semiconservative synthesis of single-stranded RNA by bacteriophage phi 6 RNA polymerase.

J L Van Etten, D E Burbank, D A Cuppels, L C Lane, A K Vidaver
PMCID: PMC288602  PMID: 7411690

Abstract

The RNA polymerase in the nucleocapsid of Pseudomonas phaseolicola bacteriophage phi 6 transcribed large, medium, and small single-stranded RNA from the viral double-stranded RNA genome by a semiconservative (displacement) mechanism. Approximately 23%, 63%, and 65% of the nucleocapsid particles in the assay mixture synthesized at least one round of large, medium, and small single-stranded RNA molecules, respectively. Some of these particles reinitiated synthesis such that an average of 1.5 large, 33 medium, and 24 small single-stranded RNAs were synthesized from each double-stranded RNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bishop D. H., Claybrook J. R., Spiegelman S. Electrophoretic separation of viral nucleic acids on polyacrylamide gels. J Mol Biol. 1967 Jun 28;26(3):373–387. doi: 10.1016/0022-2836(67)90310-5. [DOI] [PubMed] [Google Scholar]
  2. Bonner W. M., Laskey R. A. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. Eur J Biochem. 1974 Jul 1;46(1):83–88. doi: 10.1111/j.1432-1033.1974.tb03599.x. [DOI] [PubMed] [Google Scholar]
  3. Brakke M. K., Van Pelt N. Linear-log sucrose gradients for estimating sedimentation coefficients of plant viruses and nucleic acids. Anal Biochem. 1970 Nov;38(1):56–64. doi: 10.1016/0003-2697(70)90155-7. [DOI] [PubMed] [Google Scholar]
  4. Buck K. W. Semi-conservative replication of double-stranded RNA by a virion-associated RNA polymerase. Biochem Biophys Res Commun. 1978 Oct 16;84(3):639–645. doi: 10.1016/0006-291x(78)90753-2. [DOI] [PubMed] [Google Scholar]
  5. Coplin D. L., Van Etten J. L., Koski R. K., Vidaver A. K. Intermediates in the biosynthesis of double-stranded ribonucleic acids of bacteriophage phi 6. Proc Natl Acad Sci U S A. 1975 Mar;72(3):849–853. doi: 10.1073/pnas.72.3.849. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Coplin D. L., Van Etten J. L., Vidaver A. K. Synthesis of bacteriophage phi6 double-stranded ribonucleic acid. J Gen Virol. 1976 Dec;33(3):509–512. doi: 10.1099/0022-1317-33-3-509. [DOI] [PubMed] [Google Scholar]
  7. Dahlberg A. E., Dingman C. W., Peacock A. C. Electrophoretic characterization of bacterial polyribosomes in agarose-acrylamide composite gels. J Mol Biol. 1969 Apr 14;41(1):139–147. doi: 10.1016/0022-2836(69)90131-4. [DOI] [PubMed] [Google Scholar]
  8. Etten J. V., Lane L., Gonzalez C., Partridge J., Vidaver A. Comparative properties of bacteriophage phi6 and phi6 nucleocapsid. J Virol. 1976 May;18(2):652–658. doi: 10.1128/jvi.18.2.652-658.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Laskey R. A., Mills A. D. Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography. Eur J Biochem. 1975 Aug 15;56(2):335–341. doi: 10.1111/j.1432-1033.1975.tb02238.x. [DOI] [PubMed] [Google Scholar]
  10. Ratti G., Buck K. W. Semi-conservative transcription in particles of a double-stranded RNA mycovirus. Nucleic Acids Res. 1978 Oct;5(10):3843–3854. doi: 10.1093/nar/5.10.3843. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Rimon A., Haselkorn R. Transcription and replication of bacteriophage phi6 RNA. Virology. 1978 Aug;89(1):206–217. doi: 10.1016/0042-6822(78)90053-3. [DOI] [PubMed] [Google Scholar]
  12. Semancik J. S., Vidaver A. K., Van Etten J. L. Characterization of segmented double-helical RNA from bacteriophage phi6. J Mol Biol. 1973 Aug 25;78(4):617–625. doi: 10.1016/0022-2836(73)90283-0. [DOI] [PubMed] [Google Scholar]
  13. Sinclair J. F., Mindich L. RNA synthesis during infection with bacteriophage phi6. Virology. 1976 Nov;75(1):209–217. doi: 10.1016/0042-6822(76)90019-2. [DOI] [PubMed] [Google Scholar]
  14. Sinclair J. F., Tzagoloff A., Levine D., Mindich L. Proteins of bacteriophage phi6. J Virol. 1975 Sep;16(3):685–695. doi: 10.1128/jvi.16.3.685-695.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Van Etten J. L., Vidaver A. K., Koski R. K., Burnett J. P. Base composition and hybridization studies of the three double-stranded RNA segments of bacteriophage phi 6. J Virol. 1974 Jun;13(6):1254–1262. doi: 10.1128/jvi.13.6.1254-1262.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Van Etten J. L., Vidaver A. K., Koski R. K., Semancik J. S. RNA polymerase activity associated with bacteriophage phi 6. J Virol. 1973 Sep;12(3):464–471. doi: 10.1128/jvi.12.3.464-471.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Vidaver A. K., Koski R. K., Van Etten J. L. Bacteriophage phi6: a Lipid-Containing Virus of Pseudomonas phaseolicola. J Virol. 1973 May;11(5):799–805. doi: 10.1128/jvi.11.5.799-805.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]

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