Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1980 Mar;33(3):969–975. doi: 10.1128/jvi.33.3.969-975.1980

Possible involvement of a calcium-stimulated ATP-hydrolyzing activity associated with mycobacteriophage I3 in the DNA injection process.

S S Karnik, K P Gopinathan
PMCID: PMC288630  PMID: 6445012

Abstract

Ca2+ ions are necessary for the successful propagation of mycobacteriophage I3. An assay for the phage DNA release in the presence of an isolated cell wall preparation from the host was established, and in this system Ca2+ ions also stimulated the release of DNA. The inhibition of phage DNA injection caused by Tween 80 (polyoxyethylene sorbitan monooleate), a nonionic detergent routinely used in mycobacterial cultures, was reversed by Ca2+. The presence of a phage-associated ATP-hydrolyzing activity was demonstrated. This enzyme was stimulated by Ca2+ ions and inhibited by Tween 80. From this and the behavior of the two agents at the level of DNA injection, as well as the fact that phage I3 has a contractile tail structure, we conclude that the phage-associated ATPase is involved in the DNA injection process.

Full text

PDF
969

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BOWMAN B., Jr Quantitative studies on some mycobacterial phage-host systems. J Bacteriol. 1958 Jul;76(1):52–62. doi: 10.1128/jb.76.1.52-62.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Barksdale L., Kim K. S. Mycobacterium. Bacteriol Rev. 1977 Mar;41(1):217–372. doi: 10.1128/br.41.1.217-372.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bradley D. E. Ultrastructure of bacteriophage and bacteriocins. Bacteriol Rev. 1967 Dec;31(4):230–314. doi: 10.1128/br.31.4.230-314.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. DUKES P. P., KOZLOFF L. M. Phosphatases in bacteriophages T2, T4, and T5. J Biol Chem. 1959 Mar;234(3):534–538. [PubMed] [Google Scholar]
  5. Gadagkar R. R., Gopinathan K. P. Inhibition of DNA injection from mycobacteriophage I3 by Tween-80. Virology. 1978 Dec;91(2):487–488. doi: 10.1016/0042-6822(78)90396-3. [DOI] [PubMed] [Google Scholar]
  6. Grover G., Dhariwal K. R., Venkitasubramanian T. A. Studies on Mg2+-(Ca2+-)activated adenosine triphosphatase from Mycobacterium smegmatis CDC 46. J Gen Microbiol. 1978 Apr;105(2):343–345. doi: 10.1099/00221287-105-2-343. [DOI] [PubMed] [Google Scholar]
  7. KOZLOFF L. M., LUTE M. A contractile protein in the tail of bacteriophage T2. J Biol Chem. 1959 Mar;234(3):539–546. [PubMed] [Google Scholar]
  8. Kimmich G. A., Randles J., Brand J. S. Assay of picomole amounts of ATP, ADP, and AMP using the luciferase enzyme system. Anal Biochem. 1975 Nov;69(1):187–206. doi: 10.1016/0003-2697(75)90580-1. [DOI] [PubMed] [Google Scholar]
  9. Kozloff L. M., Sundar Raj C. V., Rao R. N., Chapman V. A., DeLong S. Structure of a transducing mycobacteriophage. J Virol. 1972 Feb;9(2):390–393. doi: 10.1128/jvi.9.2.390-393.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Raj C. V., Ramakrishnan T. Transduction in Mycobacterium smegmatis. Nature. 1970 Oct 17;228(5268):280–281. doi: 10.1038/228280b0. [DOI] [PubMed] [Google Scholar]
  11. Reinhardt A., Cadden S., Sands J. A. Inhibitory effect of fatty acids on the entry of the lipid-containing bacteriophage PR4 into Escherichia coli. J Virol. 1978 Feb;25(2):479–485. doi: 10.1128/jvi.25.2.479-485.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Sastry P. S., Ganguly J. Studies on vitamin A esterase. 5. A comparative study of vitamin A esterase and cholesterol esterase of rat and chicken liver. Biochem J. 1961 Aug;80(2):397–406. doi: 10.1042/bj0800397. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Simon L. D., Anderson T. F. The infection of Escherichia coli by T2 and T4 bacteriophages as seen in the electron microscope. I. Attachment and penetration. Virology. 1967 Jun;32(2):279–297. doi: 10.1016/0042-6822(67)90277-2. [DOI] [PubMed] [Google Scholar]
  14. WAHL R., KOZLOFF L. M. The nucleoside triphosphate content of various bacteriophages. J Biol Chem. 1962 Jun;237:1953–1960. [PubMed] [Google Scholar]
  15. YAMAGUCHI T. COMPARISON OF THE CELL-WALL COMPOSITION OF MORPHOLOGICALLY DISTINCT ACTINOMYCETES. J Bacteriol. 1965 Feb;89:444–453. doi: 10.1128/jb.89.2.444-453.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES