Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1980 Mar;33(3):1004–1012. doi: 10.1128/jvi.33.3.1004-1012.1980

Complementation analysis of measles virus mutants isolated from persistently infected lymphoblastoid cell lines.

G Ju, M Birrer, S Udem, B R Bloom
PMCID: PMC288634  PMID: 7365873

Abstract

Human lymphoblastoid cell lines persistently infected with measles virus release a heterogeneous population of virions. At least 80% of the infectious particles were temperature sensitive for plaque formation at 39 degrees C. Plaque-purified temperature-sensitive mutants from four persistently infected human lymphoblastoid cell lines were shown to be heterogeneous with respect to efficiency of plating at 31 and 39 degrees C, as well as to antigen and RNA production at 39 degrees C. The heterogeneity was confirmed by complementation analysis in which 21 temperature-sensitive isolates were found to represent at least four of the five previously described complementation groups of measles virus. Two isolates complemented four reference temperature-sensitive mutants. These isolates either represent new complementation groups or are members of the fifth complementation group, group E. The majority of isolates were found to have multiple mutations, and group B mutants (RNA-) predominated. Two temperature-sensitive isolates were able to interfere with production of parental measles virus at both permissive and nonpermissive temperatures.

Full text

PDF
1004

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ahmed R., Graham A. F. Persistent infections in L cells with temperature-sensitive mutants of reovirus. J Virol. 1977 Aug;23(2):250–262. doi: 10.1128/jvi.23.2.250-262.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Barry D. W., Sullivan J. L., Lucas S. J., Dunlap R. C., Albrecht P. Acute and chronic infection of human lymphoblastoid cell lines with measles virus. J Immunol. 1976 Jan;116(1):89–98. [PubMed] [Google Scholar]
  3. Bergholz C. M., Kiley M. P., Payne F. E. Isolation and characterization of temperature-sensitive mutants of measles virus. J Virol. 1975 Jul;16(1):192–202. doi: 10.1128/jvi.16.1.192-202.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bloom B. R., Ju G., Brosnan C., Cammer W., Norton W. Notes on the pathogenesis of multiple sclerosis. Neurology. 1978 Sep;28(9 Pt 2):93–101. doi: 10.1212/wnl.28.9_part_2.93. [DOI] [PubMed] [Google Scholar]
  5. Bloom B. R., Senik A., Stoner G., Ju G., Nowakowski M., Kano S., Jimenez L. Studies on the interactions between viruses and lymphocytes. Cold Spring Harb Symp Quant Biol. 1977;41(Pt 1):73–83. doi: 10.1101/sqb.1977.041.01.011. [DOI] [PubMed] [Google Scholar]
  6. Breschkin A. M., Rapp F., Payne F. E. Complementation analysis of measles virus temperature-sensitive mutants. J Virol. 1977 Jan;21(1):439–441. doi: 10.1128/jvi.21.1.439-441.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Buckton K. E., Brown W. M., Smith P. G. Lymphocyte survival in men treated with x-rays for ankylosing spondylitis. Nature. 1967 Apr 29;214(5087):470–473. doi: 10.1038/214470a0. [DOI] [PubMed] [Google Scholar]
  8. Desmyter J., Melnick J. L., Rawls W. E. Defectiveness of interferon production and of rubella virus interference in a line of African green monkey kidney cells (Vero). J Virol. 1968 Oct;2(10):955–961. doi: 10.1128/jvi.2.10.955-961.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hall W. W., Martin S. J. Purification and characterization of measles virus. J Gen Virol. 1973 May;19(2):175–188. doi: 10.1099/0022-1317-19-2-175. [DOI] [PubMed] [Google Scholar]
  10. Haspel M. V., Duff R., Rapp F. Isolation and preliminary characterization of temperature-sensitive mutants of measles virus. J Virol. 1975 Oct;16(4):1000–1009. doi: 10.1128/jvi.16.4.1000-1009.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Holland J. J., Villarreal L. P., Welsh R. M., Oldstone M. B., Kohne D., Lazzarini R., Scolnick E. Long-term persistent vesicular stomatitis virus and rabies virus infection of cells in vitro. J Gen Virol. 1976 Nov;33(2):193–211. doi: 10.1099/0022-1317-33-2-193. [DOI] [PubMed] [Google Scholar]
  12. Horta-Barbosa L., Hamilton R., Wittig B., Fuccillo D. A., Sever J. L., Vernon M. L. Subacute sclerosing panencephalitis: isolation of suppressed measles virus from lymph node biopsies. Science. 1971 Aug 27;173(3999):840–841. doi: 10.1126/science.173.3999.840. [DOI] [PubMed] [Google Scholar]
  13. Inglot A. D., Albin M., Chudzio T. Persistent infection of mouse cells with Sindbis virus: role of virulence of strains, auto-interfering particles and interferon. J Gen Virol. 1973 Jul;20(1):105–110. doi: 10.1099/0022-1317-20-1-105. [DOI] [PubMed] [Google Scholar]
  14. Ju G., Udem S., Rager-Zisman B., Bloom B. R. Isolation of a heterogeneous population of temperature-sensitive mutants of measles virus from persistently infected human lymphoblastoid cell lines. J Exp Med. 1978 Jun 1;147(6):1637–1652. doi: 10.1084/jem.147.6.1637. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. McGuire T. C., Crawford T. B., Henson J. B. Immunofluorescent localization of equine infectious anemia virus in tissue. Am J Pathol. 1971 Feb;62(2):283–294. [PMC free article] [PubMed] [Google Scholar]
  16. Morgan E. M., Rapp F. Measles virus and its associated diseases. Bacteriol Rev. 1977 Sep;41(3):636–666. doi: 10.1128/br.41.3.636-666.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Narayan O., Griffin D. E., Chase J. Antigenic shift of visna virus in persistently infected sheep. Science. 1977 Jul 22;197(4301):376–378. doi: 10.1126/science.195339. [DOI] [PubMed] [Google Scholar]
  18. Preble O. T., Youngner J. S. Temperature-sensitive defect of mutants isolated from L cells persistently infected with Newcastle disease virus. J Virol. 1973 Sep;12(3):472–480. doi: 10.1128/jvi.12.3.472-480.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Preble O. T., Youngner J. S. Temperature-sensitive viruses and the etiology of chronic and inapparent infections. J Infect Dis. 1975 Apr;131(4):467–473. doi: 10.1093/infdis/131.4.467. [DOI] [PubMed] [Google Scholar]
  20. Pétursson G., Nathanson N., Georgsson G., Panitch H., Pálsson P. A. Pathogenesis of visna. I. Sequential virologic, serologic, and pathologic studies. Lab Invest. 1976 Oct;35(4):402–412. [PubMed] [Google Scholar]
  21. Rima R. K., Martin S. J. Persistent infection of tissue culture cells by RNA viruses. Med Microbiol Immunol. 1976 Jun 1;162(2):89–119. doi: 10.1007/BF02121320. [DOI] [PubMed] [Google Scholar]
  22. Simpson R. W., Iinuma M. Recovery of infectious proviral DNA from mammalian cells infected with respiratory syncytial virus. Proc Natl Acad Sci U S A. 1975 Aug;72(8):3230–3234. doi: 10.1073/pnas.72.8.3230. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. WALKER D. L. THE VIRAL CARRIER STATE IN ANIMAL CELL CULTURES. Prog Med Virol. 1964;6:111–148. [PubMed] [Google Scholar]
  24. Wiktor T. J., Clark H. F. Chronic rabies virus infection of cell cultures. Infect Immun. 1972 Dec;6(6):988–995. doi: 10.1128/iai.6.6.988-995.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Youngner J. S., Preble O. T., Jones E. V. Persistent infection of L cells with vesicular stomatitis virus: evolution of virus populations. J Virol. 1978 Oct;28(1):6–12. doi: 10.1128/jvi.28.1.6-13.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Youngner J. S., Quagliana D. O. Temperature-sensitive mutants of vesicular stomatitis virus are conditionally defective particles that interfere with and are rescued by wild-type virus. J Virol. 1976 Jul;19(1):102–107. doi: 10.1128/jvi.19.1.102-107.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Zhdanov V. M. Integration of viral genomes. Nature. 1975 Aug 7;256(5517):471–473. doi: 10.1038/256471a0. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES