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. 1980 Mar;33(3):1203–1207. doi: 10.1128/jvi.33.3.1203-1207.1980

Purification and preliminary immunological characterization of the type 5 adenovirus, nonstructural 100,000-dalton protein.

E A Oosterom-Dragon, H S Ginsberg
PMCID: PMC288654  PMID: 6988609

Abstract

The nonstructural 100,000-dalton (100K) protein of type 5 adenovirus was isolated and purified from infected KB cells by a combination of ion-exchange and affinity chromatographies. Rabbit antiserum containing specific 100K protein antibodies was used for indirect immunofluorescence examination of cells infected with wild-type virus, 100K mutants, and hexon mutants. The 100K protein, which is synthesized as a late protein, was observed primarily in the cytoplasm of cells infected with wild-type and mutant viruses.

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Selected References

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