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. 1980 Apr;34(1):9–27. doi: 10.1128/jvi.34.1.9-27.1980

Characterization of Herpes Simplex Virus Type 1 RNA Present in the Absence of De Novo Protein Synthesis

Kevin P Anderson 1, Robert H Costa 1, Louis E Holland 1, Edward K Wagner 1
PMCID: PMC288666  PMID: 6246265

Abstract

We used herpes simplex virus type 1 (HSV-1) DNA and restriction fragments of HSV-1 DNA covalently coupled to cellulose as a reagent to isolate for further characterization the major and minor HSV-1 immediate-early mRNA species in HeLa cells infected and maintained in the absence of de novo protein synthesis. Five major and several minor immediate-early mRNA species were characterized. One major species was a 4.2-kilobase mRNA mapping in the TRS/IRS region with its 3′ end distal to the US region; this mRNA encoded a 170,000-dalton polypeptide in vitro. A 2.8-kilobase mRNA, encoding a 120,000-dalton polypeptide, was mapped in the TRL/IRL region with its 3′ end directed toward the UL region. Three 1.8-kilobase mRNA species were mapped. One, mapping in the IRS region with its 3′ end in the US, encoded a 68,000-dalton polypeptide. One mapped in the TRS region and had its 3′ end in the US region; the third one encoded a 64,000-dalton polypeptide and mapped in the UL region near the IRL region. One minor species 5.2 kilobases in size was clearly detectable mapping in the UL region. Furthermore, there were indications that one or more immediate-early mRNA species approximately 3 kilobases in size hybridized to regions near the TRL and in or near the TRS/IRS regions. Nuclear immediate-early RNA mapped only in those regions where polyribosomal immediate-early mRNA mapped, although minor differences were seen. Finally, we demonstrated that at least three major immediate-early mRNA's—4.2 kilobases, 2.8 kilobases, and the 1.8-kilobase one mapping in the IRS/US region—continued to appear on polyribosomes as functional mRNA late after infection.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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