Abstract
Treatment of Newcastle disease virus with lithium diiodosalicylate differentially elutes the internally disposed proteins, M and NP, showing that these proteins are extrinsic, i.e., not associated with the lipid hydrophobic core. This selective elution requires disruption of the viral envelope, a process that is maximal at low temperature and influenced by the lipid composition of the virus envelope.
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- Anker H. S. A solubilizable acrylamide gel for electrophoresis. FEBS Lett. 1970 Apr 16;7(3):293–293. doi: 10.1016/0014-5793(70)80185-5. [DOI] [PubMed] [Google Scholar]
- Hightower L. E., Morrison T. G., Bratt M. A. Relationships among the polypeptides of Newcastle disease virus. J Virol. 1975 Dec;16(6):1599–1607. doi: 10.1128/jvi.16.6.1599-1607.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Lamb R. A., Choppin P. W. The synthesis of Sendai virus polypeptides in infected cells. II. Intracellular distribution of polypeptides. Virology. 1977 Sep;81(2):371–381. doi: 10.1016/0042-6822(77)90153-2. [DOI] [PubMed] [Google Scholar]
- Lenard J., Compans R. W. The membrane structure of lipid-containing viruses. Biochim Biophys Acta. 1974 Apr 8;344(1):51–94. doi: 10.1016/0304-4157(74)90008-2. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Li J. K., Fox C. F. Radioiodination of the envelope proteins of Newcastle disease virus. J Supramol Struct. 1975;3(1):51–60. doi: 10.1002/jss.400030106. [DOI] [PubMed] [Google Scholar]
- Li J. K., Williams R. E., Fox C. F. Effects of temperature and host lipid composition on the infection of cells by Newcastle disease virus. Biochem Biophys Res Commun. 1975 Jan 20;62(2):470–477. doi: 10.1016/s0006-291x(75)80162-8. [DOI] [PubMed] [Google Scholar]
- Markwell M. A., Fox C. F. Protein-protein interactions within paramyxoviruses identified by native disulfide bonding or reversible chemical cross-linking. J Virol. 1980 Jan;33(1):152–166. doi: 10.1128/jvi.33.1.152-166.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Markwell M. A., Fox C. F. Surface-specific iodination of membrane proteins of viruses and eucaryotic cells using 1,3,4,6-tetrachloro-3alpha,6alpha-diphenylglycoluril. Biochemistry. 1978 Oct 31;17(22):4807–4817. doi: 10.1021/bi00615a031. [DOI] [PubMed] [Google Scholar]
- Mountcastle W. E., Compans R. W., Caliguiri L. A., Choppin P. W. Nucleocapsid protein subunits of simian virus 5, Newcastle disease virus, and Sendai virus. J Virol. 1970 Nov;6(5):677–684. doi: 10.1128/jvi.6.5.677-684.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mountcastle W. E., Compans R. W., Choppin P. W. Proteins and glycoproteins of paramyxoviruses: a comparison of simian virus 5, Newcastle disease virus, and Sendai virus. J Virol. 1971 Jan;7(1):47–52. doi: 10.1128/jvi.7.1.47-52.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nagai Y., Klenk H. D., Rott R. Proteolytic cleavage of the viral glycoproteins and its significance for the virulence of Newcastle disease virus. Virology. 1976 Jul 15;72(2):494–508. doi: 10.1016/0042-6822(76)90178-1. [DOI] [PubMed] [Google Scholar]
- Nagai Y., Ogura H., Klenk H. Studies on the assembly of the envelope of Newcastle disease virus. Virology. 1976 Feb;69(2):523–538. doi: 10.1016/0042-6822(76)90482-7. [DOI] [PubMed] [Google Scholar]
- Scheid A., Choppin P. W. Identification of biological activities of paramyxovirus glycoproteins. Activation of cell fusion, hemolysis, and infectivity of proteolytic cleavage of an inactive precursor protein of Sendai virus. Virology. 1974 Feb;57(2):475–490. doi: 10.1016/0042-6822(74)90187-1. [DOI] [PubMed] [Google Scholar]
- Scheid A., Choppin P. W. Isolation and purification of the envelope proteins of Newcastle disease virus. J Virol. 1973 Feb;11(2):263–271. doi: 10.1128/jvi.11.2.263-271.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shapiro S. C., Bratt M. S. Proteins of four biologically distinct strains of Newcastle disease virus. Proc Soc Exp Biol Med. 1971 Mar;136(3):834–838. doi: 10.3181/00379727-136-35375. [DOI] [PubMed] [Google Scholar]
- Steck T. L. The organization of proteins in the human red blood cell membrane. A review. J Cell Biol. 1974 Jul;62(1):1–19. doi: 10.1083/jcb.62.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Steck T. L., Yu J. Selective solubilization of proteins from red blood cell membranes by protein perturbants. J Supramol Struct. 1973;1(3):220–232. doi: 10.1002/jss.400010307. [DOI] [PubMed] [Google Scholar]
- Williams V. P., Freidenreich P., Glazer A. N. Homology of amino-terminal regions of C-phycocyanins from a prokaryote and a eukaryote. Biochem Biophys Res Commun. 1974 Jul 24;59(2):462–466. doi: 10.1016/s0006-291x(74)80002-1. [DOI] [PubMed] [Google Scholar]
- Wisnieski B. J., Parkes J. G., Huang Y. O., Fox C. F. Physical and physiological evidence for two phase transitions in cytoplasmic membranes of animal cells. Proc Natl Acad Sci U S A. 1974 Nov;71(11):4381–4385. doi: 10.1073/pnas.71.11.4381. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yoshida T., Nagai Y., Maeno K., Iinuma M., Hamaguchi M., Matsumoto T., Nagayoshi S., Hoshino M. Studies on the role of M protein in virus assembly using a ts mutant of HVJ (Sendai virus). Virology. 1979 Jan 15;92(1):139–154. doi: 10.1016/0042-6822(79)90220-4. [DOI] [PubMed] [Google Scholar]
- Yu J., Fischman D. A., Steck T. L. Selective solubilization of proteins and phospholipids from red blood cell membranes by nonionic detergents. J Supramol Struct. 1973;1(3):233–248. doi: 10.1002/jss.400010308. [DOI] [PubMed] [Google Scholar]


