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. 1980 Apr;34(1):293–296. doi: 10.1128/jvi.34.1.293-296.1980

Specificity of protein synthesis inhibitors in the inhibition of encephalomyocarditis virus replication.

T V Ramabhadran, R E Thach
PMCID: PMC288699  PMID: 6246261

Abstract

Effect of protein synthesis inhibitors on encephalomyocarditis virus production in L-cells was studied. Inhibition of initiation by hypertonicity, harringtonine, or pactamycin decreased viral protein synthesis to a lesser extent than that of host. Virus yield was unaffected or actually enhanced by low concentrations of these inhibitors. On the contrary, the elongation inhibitors cycloheximide, anisomycin, and emetine, shown previously to inhibit viral protein synthesis preferentially, had a greater effect on virus yield than on overall protein synthesis. These results support our earlier proposal that the antiviral activity of cycloheximide derives from its specific effect on the rate of elongation of protein synthesis, and that elongation inhibitors in general may show varying degrees of specific antiviral activity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Fresno M., Jiménez A., Vázquez D. Inhibition of translation in eukaryotic systems by harringtonine. Eur J Biochem. 1977 Jan;72(2):323–330. doi: 10.1111/j.1432-1033.1977.tb11256.x. [DOI] [PubMed] [Google Scholar]
  2. Golini F., Thach S. S., Birge C. H., Safer B., Merrick W. C., Thach R. E. Competition between cellular and viral mRNAs in vitro is regulated by a messenger discriminatory initiation factor. Proc Natl Acad Sci U S A. 1976 Sep;73(9):3040–3044. doi: 10.1073/pnas.73.9.3040. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Grollman A. P., Huang M. T. Inhibitors of protein synthesis in eukaryotes: tools in cell research. Fed Proc. 1973 Jun;32(6):1673–1678. [PubMed] [Google Scholar]
  4. Huang M. T. Harringtonine, an inhibitor of initiation of protein biosynthesis. Mol Pharmacol. 1975 Sep;11(5):511–519. [PubMed] [Google Scholar]
  5. Jen G., Birge C. H., Thach R. E. Comparison of initiation rates of encephalomyocarditis virus and host protein synthesis in infected cells. J Virol. 1978 Sep;27(3):640–647. doi: 10.1128/jvi.27.3.640-647.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Lawrence C., Thach R. E. Encephalomyocarditis virus infection of mouse plasmacytoma cells. I. Inhibition of cellular protein synthesis. J Virol. 1974 Sep;14(3):598–610. doi: 10.1128/jvi.14.3.598-610.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Lodish H. F. Alpha and beta globin messenger ribonucleic acid. Different amounts and rates of initiation of translation. J Biol Chem. 1971 Dec 10;246(23):7131–7138. [PubMed] [Google Scholar]
  8. Lodish H. F. Model for the regulation of mRNA translation applied to haemoglobin synthesis. Nature. 1974 Oct 4;251(5474):385–388. doi: 10.1038/251385a0. [DOI] [PubMed] [Google Scholar]
  9. Nuss D. L., Koch G. Differential inhibition of vesicular stomatitis virus polypeptide synthesis by hypertonic initiation block. J Virol. 1975 Jan;17(1):283–286. doi: 10.1128/jvi.17.1.283-286.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Nuss D. L., Oppermann H., Koch G. Selective blockage of initiation of host protein synthesis in RNA-virus-infected cells. Proc Natl Acad Sci U S A. 1975 Apr;72(4):1258–1262. doi: 10.1073/pnas.72.4.1258. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Oppermann H., Koch G. On the regulation of protein synthesis in vaccinia virus infected cells. J Gen Virol. 1976 Aug;32(2):261–273. doi: 10.1099/0022-1317-32-2-261. [DOI] [PubMed] [Google Scholar]
  12. Saborio J. L., Pong S. S., Koch G. Selective and reversible inhibition of initiation of protein synthesis in mammalian cells. J Mol Biol. 1974 May 15;85(2):195–211. doi: 10.1016/0022-2836(74)90360-x. [DOI] [PubMed] [Google Scholar]
  13. Tscherne J. S., Pestka S. Inhibition of protein synthesis in intact HeLa cells. Antimicrob Agents Chemother. 1975 Oct;8(4):479–487. doi: 10.1128/aac.8.4.479. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Vaughan M. H., Hansen B. S. Control of initiation of protein synthesis in human cells. Evidence for a role of uncharged transfer ribonucleic acid. J Biol Chem. 1973 Oct 25;248(20):7087–7096. [PubMed] [Google Scholar]
  15. Yau P. M., Godefroy-Colburn T., Birge C. H., Ramabhadran T. V., Thach R. E. Specificity of interferon action in protein synthesis. J Virol. 1978 Sep;27(3):648–658. doi: 10.1128/jvi.27.3.648-658.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]

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