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. 1980 May;34(2):532–541. doi: 10.1128/jvi.34.2.532-541.1980

Expression of Wound Tumor Virus Gene Products In Vivo and In Vitro

Donald L Nuss 1, Andrew J Peterson 1
PMCID: PMC288733  PMID: 16789196

Abstract

Infection of Agallia constricta vector cell monolayers with wound tumor virus results in the synthesis of 12 virus-specific polypeptides. Confirmation that these polypeptides are virus encoded rather than virus induced was obtained by cell-free translation of in vitro synthesized viral mRNA. In addition, transcription by purified wound tumor virus particles was coupled with translation of the resulting transcripts in a wheat embryo cell-free extract. Six previously described structural polypeptides, one presumptive structural polypeptide, and five previously unidentified nonstructural polypeptides were synthesized in infected vector cell monolayers, in cell-free extracts directed by in vitro synthesized viral mRNA, and in the homologous plant cell-free system, in which viral transcription was coupled with translation. Pulse-chase experiments revealed no evidence of precursor-product relationships for the wound tumor virus-specific polypeptides.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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