Abstract
Crude initiation factor preparations from poliovirus-infected cells stimulated the translation of poliovirus RNA in vitro, but were inactive for the translation of host cell or vesicular stomatitis virus mRNA's. In contrast, similar preparations from either uninfected or vesicular stomatitis virus-infected cells supported the initiation of translation of host cell mRNA's and both viral mRNA's. These results reflect a specific alteration of some components(s) of the initiation factor preparation from poliovirus-infected cells which is consistent with the ability of the virus to inhibit the translation of host cell and vesicular stomatitis virus-directed protein synthesis.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Brown B. A., Ehrenfeld E. Translation of poliovirus RNA in vitro: changes in cleavage pattern and initiation sites by ribosomal salt wash. Virology. 1979 Sep;97(2):396–405. doi: 10.1016/0042-6822(79)90350-7. [DOI] [PubMed] [Google Scholar]
- Celma M. L., Ehrenfeld E. Effect of poliovirus double-stranded RNA on viral and host-cell protein synthesis. Proc Natl Acad Sci U S A. 1974 Jun;71(6):2440–2444. doi: 10.1073/pnas.71.6.2440. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Celma M. L., Ehrenfeld E. Translation of poliovirus RNA in vitro: detection of two different initiation sites. J Mol Biol. 1975 Nov 15;98(4):761–780. doi: 10.1016/s0022-2836(75)80009-x. [DOI] [PubMed] [Google Scholar]
- Chamberlain J. P. Fluorographic detection of radioactivity in polyacrylamide gels with the water-soluble fluor, sodium salicylate. Anal Biochem. 1979 Sep 15;98(1):132–135. doi: 10.1016/0003-2697(79)90716-4. [DOI] [PubMed] [Google Scholar]
- Doyle M., Holland J. J. Virus-induced interference in heterologously infected HeLa cells. J Virol. 1972 Jan;9(1):22–28. doi: 10.1128/jvi.9.1.22-28.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ehrenfeld E., Lund H. Untranslated vesicular stomatitis virus messenger RNA after poliovirus infection. Virology. 1977 Jul 15;80(2):297–308. doi: 10.1016/s0042-6822(77)80006-8. [DOI] [PubMed] [Google Scholar]
- Helentjaris T., Ehrenfeld E., Brown-Luedi M. L., Hershey J. W. Alterations in initiation factor activity from poliovirus-infected HeLa cells. J Biol Chem. 1979 Nov 10;254(21):10973–10978. [PubMed] [Google Scholar]
- Helentjaris T., Ehrenfeld E. Control of protein synthesis in extracts from poliovirus-infected cells. I. mRNA discrimination by crude initiation factors. J Virol. 1978 May;26(2):510–521. doi: 10.1128/jvi.26.2.510-521.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Humphries S., Knauert F., Ehrenfeld E. Capsid protein precursor is one of two initiated products of translation of poliovirus RNA in vitro. J Virol. 1979 May;30(2):481–488. doi: 10.1128/jvi.30.2.481-488.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Knauert F., Ehrenfeld E. Translation of poliovirus RNA in vitro: studies on n-formylmethionine-labeled polypeptides initiated in cell-free extracts prepared from poliovirus infected HeLa cells. Virology. 1979 Mar;93(2):537–546. doi: 10.1016/0042-6822(79)90256-3. [DOI] [PubMed] [Google Scholar]
- Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
- Pelham H. R., Jackson R. J. An efficient mRNA-dependent translation system from reticulocyte lysates. Eur J Biochem. 1976 Aug 1;67(1):247–256. doi: 10.1111/j.1432-1033.1976.tb10656.x. [DOI] [PubMed] [Google Scholar]
- Rose J. K., Trachsel H., Leong K., Baltimore D. Inhibition of translation by poliovirus: inactivation of a specific initiation factor. Proc Natl Acad Sci U S A. 1978 Jun;75(6):2732–2736. doi: 10.1073/pnas.75.6.2732. [DOI] [PMC free article] [PubMed] [Google Scholar]