Abstract
The footpad skin and the lumbosacral dorsal root ganglia were removed from inbred guinea pigs at different times after subcutaneous infection with herpes simplex virus type 2 (HSV-2) in both hind footpads. These tissues, shown by our previous study to harbor latent HSV, were dispersed into single cells. The presence of virus-specific thymidine kinase (TK) in these cells was assayed by the uptake and phosphorylation of [125I]deoxycytidine in culture. [125I]deoxycytidine was shown to be a specific substrate for the HSV-coded TK. The method could detect herpes TK activity in a culture of 10(6) cells with less than 0.1% of the cells being virally infected. The enzyme was readily detected in footpad cells of acutely (24 h) but not of latently (14 days to 1 year) infected guinea pigs. No herpes TK was found either in the sensory ganglionic cells of guinea pigs during the early and late phases of latent infection. It is concluded that HSV-2, while residing in the footpads and the lumbosacral ganglia of the guinea pig during latent infection, does not express any viral TK function.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Baringer J. R. Recovery of herpes simplex virus from human sacral ganglions. N Engl J Med. 1974 Oct 17;291(16):828–830. doi: 10.1056/NEJM197410172911606. [DOI] [PubMed] [Google Scholar]
- Baringer J. R., Swoveland P. Recovery of herpes-simplex virus from human trigeminal ganglions. N Engl J Med. 1973 Mar 29;288(13):648–650. doi: 10.1056/NEJM197303292881303. [DOI] [PubMed] [Google Scholar]
- Bastian F. O., Rabson A. S., Yee C. L., Tralka T. S. Herpesvirus hominis: isolation from human trigeminal ganglion. Science. 1972 Oct 20;178(4058):306–307. doi: 10.1126/science.178.4058.306. [DOI] [PubMed] [Google Scholar]
- Bittlingmaier K., Schneider D., Falke D. Thymidine transport in herpesvirus hominis type 1 and 2 infected BHK 21 cells. J Gen Virol. 1977 Apr;35(1):159–173. doi: 10.1099/0022-1317-35-1-159. [DOI] [PubMed] [Google Scholar]
- Galloway D. A., Fenoglio C., Shevchuk M., McDougall J. K. Detection of herpes simplex RNA in human sensory ganglia. Virology. 1979 May;95(1):265–268. doi: 10.1016/0042-6822(79)90429-x. [DOI] [PubMed] [Google Scholar]
- Jamieson A. T., Subak-Shape J. H. Herpes simplex virus specified deoxypyrimidine kinase and the uptake of exogenous nucleosides by infected cells. J Gen Virol. 1976 Jun;31(3):303–314. doi: 10.1099/0022-1317-31-3-303. [DOI] [PubMed] [Google Scholar]
- Knotts F. B., Cook M. L., Stevens J. G. Latent herpes simplex virus in the central nervous system of rabbits and mice. J Exp Med. 1973 Sep 1;138(3):740–744. doi: 10.1084/jem.138.3.740. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Price R. W., Katz B. J., Notkins A. L. Latent infection of the peripheral ANS with herpes simplex virus. Nature. 1975 Oct 23;257(5528):686–688. doi: 10.1038/257686a0. [DOI] [PubMed] [Google Scholar]
- Puga A., Rosenthal J. D., Openshaw H., Notkins A. L. Herpes simplex virus DNA and mRNA sequences in acutely and chronically infected trigeminal ganglia of mice. Virology. 1978 Aug;89(1):102–111. doi: 10.1016/0042-6822(78)90044-2. [DOI] [PubMed] [Google Scholar]
- Reeves W. C., DiGiacomo R. F., Alexander E. R., Lee C. K. Latent Herpesvirus hominis from trigeminal and sacral dorsal root ganglia of Cebus monkeys. Proc Soc Exp Biol Med. 1976 Nov;153(2):258–261. doi: 10.3181/00379727-153-39523. [DOI] [PubMed] [Google Scholar]
- Scriba M. Extraneural localisation of herpes simplex virus in latently infected guinea pigs. Nature. 1977 Jun 9;267(5611):529–531. doi: 10.1038/267529a0. [DOI] [PubMed] [Google Scholar]
- Scriba M. Herpes simplex virus infection in guinea pigs: an animal model for studying latent and recurrent herpes simplex virus infection. Infect Immun. 1975 Jul;12(1):162–165. doi: 10.1128/iai.12.1.162-165.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stevens J. G., Cook M. L. Latent herpes simplex virus in spinal ganglia of mice. Science. 1971 Aug 27;173(3999):843–845. doi: 10.1126/science.173.3999.843. [DOI] [PubMed] [Google Scholar]
- Stevens J. G., Nesburn A. B., Cook M. L. Latent herpes simplex virus from trigeminal ganglia of rabbits with recurrent eye infection. Nat New Biol. 1972 Feb 16;235(59):216–217. doi: 10.1038/newbio235216a0. [DOI] [PubMed] [Google Scholar]
- Summers W. C., Summers W. P. [125I]deoxycytidine used in a rapid, sensitive, and specific assay for herpes simplex virus type 1 thymidine kinase. J Virol. 1977 Oct;24(1):314–318. doi: 10.1128/jvi.24.1.314-318.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Walz M. A., Yamamoto H., Notkins A. L. Immunological response restricts number of cells in sensory ganglia infected with herpes simplex virus. Nature. 1976 Dec 9;264(5586):554–556. doi: 10.1038/264554a0. [DOI] [PubMed] [Google Scholar]
- Yamamoto H., Walz M. A., Notkins A. L. Viral-specific thymidine kinase in sensory ganglia of mice infected with herpes simplex virus. Virology. 1977 Feb;76(2):866–869. doi: 10.1016/0042-6822(77)90267-7. [DOI] [PubMed] [Google Scholar]