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Journal of Virology logoLink to Journal of Virology
. 1980 Jun;34(3):772–776. doi: 10.1128/jvi.34.3.772-776.1980

Transformation of rat cells by fusion-infection with Rous sarcoma virus.

S Kawai
PMCID: PMC288765  PMID: 6247511

Abstract

The transformation of a rat cell line, 3Y1, by nonmammalian tropic strains of avian sarcoma virus was tested using cell-virus fusion mediated by Sendai virus or polyethylene glycol. Furthermore, the establishment of several transformed 3Y1 cell clones induced by the Schmidt-Ruppin strain of Rous sarcoma virus (RSV), its derivative mutants, and the Bryan high-titer strain of RSV is reported. The presence and expression of the viral genomes in these cells were examined, and all transformed cell clones tested were found to contain rescuable RSV genomes when they had been fused with normal chicken embryo fibroblast cells or those preinfected with Rous-associated virus type 1. However, the gag gene product, pr76, was barely detectable in wild-type RSV-transformed cells, whereas it was produced in considerable amounts in cells transformed by env-deleted mutants, the Bryan high-titer strain of RSV and NY8 derived from the Schmidt-Ruppin strain of RSV.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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