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. 1980 Jul;35(1):93–104. doi: 10.1128/jvi.35.1.93-104.1980

Exclusion of bacteriophage T1 by bacteriophage lambda. II. Synthesis of T1-specific macromolecules under N-mediated excluding conditions.

M C Gawron, J R Christensen, T M Shoemaker
PMCID: PMC288785  PMID: 6447803

Abstract

The results of experiments investigating T1 macromolecular synthesis under N-mediated excluding conditions failed to demonstrate a substantial alteration in the T1 mRNA production in excluding cultures at any stage in the T1 infectious cycle. The number of T1 DNA sequences in the excluding culture was found to be one-third to one-half that found in T1-infected cultures. The most severe reduction in T1-specific macromolecules was seen in protein synthesis. Total incorporation of labeled amino acids was reduced sixfold, and gel experiments confirmed that the T1-specific proteins capable of detection are reduced in excluding cells.

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Selected References

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  1. Adhya S., Gottesman M. Control of transcription termination. Annu Rev Biochem. 1978;47:967–996. doi: 10.1146/annurev.bi.47.070178.004535. [DOI] [PubMed] [Google Scholar]
  2. Bertrand K., Squires C., Yanofsky C. Transcription termination in vivo in the leader region of the tryptophan operon of Escherichia coli. J Mol Biol. 1976 May 15;103(2):319–337. doi: 10.1016/0022-2836(76)90315-6. [DOI] [PubMed] [Google Scholar]
  3. Botchan M., Topp W., Sambrook J. The arrangement of simian virus 40 sequences in the DNA of transformed cells. Cell. 1976 Oct;9(2):269–287. doi: 10.1016/0092-8674(76)90118-5. [DOI] [PubMed] [Google Scholar]
  4. Brody E. N., Mackal R. P., Evans E. A., Jr Properties of infectious T1 deoxyribonucleic acid. J Virol. 1967 Feb;1(1):76–85. doi: 10.1128/jvi.1.1.76-85.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Christensen J. R., Gawron M. C., Halpern J. Exclusion of bacteriophage T1 by bacteriophage lambda. I. Early exclusion requires lambda N gene product and host factors involved in N gene expression. J Virol. 1978 Feb;25(2):527–534. doi: 10.1128/jvi.25.2.527-534.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Denhardt D. T. A membrane-filter technique for the detection of complementary DNA. Biochem Biophys Res Commun. 1966 Jun 13;23(5):641–646. doi: 10.1016/0006-291x(66)90447-5. [DOI] [PubMed] [Google Scholar]
  7. Figurski D. H., Christensen J. R. Functional characterization of the genes of bacteriophage T1. Virology. 1974 Jun;59(2):397–407. doi: 10.1016/0042-6822(74)90453-x. [DOI] [PubMed] [Google Scholar]
  8. Greenblatt J. Regulation of the expression of the N gene of bacteriophage lambda. Proc Natl Acad Sci U S A. 1973 Feb;70(2):421–424. doi: 10.1073/pnas.70.2.421. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hayward G. S. Gel electrophoretic separation of the complementary strands of bacteriophage DNA. Virology. 1972 Jul;49(1):342–344. doi: 10.1016/s0042-6822(72)80042-4. [DOI] [PubMed] [Google Scholar]
  10. Kasai T., Bautz E. K. Regulation of gene-specific RNA synthesis in bacteriophage T4. J Mol Biol. 1969 May 14;41(3):401–417. doi: 10.1016/0022-2836(69)90284-8. [DOI] [PubMed] [Google Scholar]
  11. LABAW L. W. The origin of phosphorus in the T1, T5, T6, and T7 bacteriophages of Escherichia coli. J Bacteriol. 1953 Oct;66(4):429–436. doi: 10.1128/jb.66.4.429-436.1953. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lodish H. F. Translational control of protein synthesis. Annu Rev Biochem. 1976;45:39–72. doi: 10.1146/annurev.bi.45.070176.000351. [DOI] [PubMed] [Google Scholar]
  13. Martin D. T., Adair C. A., Ritchie D. A. Polypeptides specified by bacteriophage T1. J Gen Virol. 1976 Nov;33(2):309–319. doi: 10.1099/0022-1317-33-2-309. [DOI] [PubMed] [Google Scholar]
  14. Morse D. E., Yanofsky C. A transcription-initiating mutation within a structural gene of the tryptophan operon. J Mol Biol. 1969 May 14;41(3):317–328. doi: 10.1016/0022-2836(69)90278-2. [DOI] [PubMed] [Google Scholar]
  15. Puga A., Borrás M. T., Tessman E. S., Tessman I. Difference between functional and structural integrity of messenger RNA. Proc Natl Acad Sci U S A. 1973 Jul;70(7):2171–2175. doi: 10.1073/pnas.70.7.2171. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Ritchie D. A., Joicey D. H. Formation of concatemeric DNA as an intermediate in the replication of bacteriophage T1 DNA molecules. J Gen Virol. 1978 Dec;41(3):609–622. doi: 10.1099/0022-1317-41-3-609. [DOI] [PubMed] [Google Scholar]
  17. Rose J. K., Mosteller R. D., Yanofsky C. Tryptophan messenger ribonucleic acid elongation rates and steady-state levels of tryptophan operon enzymes under various growth conditions. J Mol Biol. 1970 Aug;51(3):541–550. doi: 10.1016/0022-2836(70)90007-0. [DOI] [PubMed] [Google Scholar]
  18. Schwartz M. On the function of the N cistron in phage lambda. Virology. 1970 Jan;40(1):23–33. doi: 10.1016/0042-6822(70)90375-2. [DOI] [PubMed] [Google Scholar]
  19. Studier F. W., Maizel J. V., Jr T7-directed protein synthesis. Virology. 1969 Nov;39(3):575–586. doi: 10.1016/0042-6822(69)90105-6. [DOI] [PubMed] [Google Scholar]
  20. Studier F. W. The genetics and physiology of bacteriophage T7. Virology. 1969 Nov;39(3):562–574. doi: 10.1016/0042-6822(69)90104-4. [DOI] [PubMed] [Google Scholar]
  21. Talkington C., Pero J. Restriction fragment analysis of the temporal program of bacteriophage SPO1 transcription and its control by phage-modified RNA polymerases. Virology. 1977 Dec;83(2):365–379. doi: 10.1016/0042-6822(77)90181-7. [DOI] [PubMed] [Google Scholar]
  22. WAITES W. M., FRY B. A. EFFECT OF INFECTION WITH PHAGE LAMBDA ON THE SYNTHESIS OF PROTEIN, RNA AND DNA IN ESCHERICHIA COLI. J Gen Microbiol. 1964 Mar;34:413–426. doi: 10.1099/00221287-34-3-413. [DOI] [PubMed] [Google Scholar]
  23. Wagner E. F., Ponta H., Schweiger M. Development of E.coli virus T1: the pattern of gene expression. Mol Gen Genet. 1977 Jan 7;150(1):21–28. doi: 10.1007/BF02425321. [DOI] [PubMed] [Google Scholar]
  24. Wagner E. F., Ponta H., Schweiger M. Development of Escherichia coli virus T1: repression of host gene expression. Eur J Biochem. 1977 Oct 17;80(1):255–260. doi: 10.1111/j.1432-1033.1977.tb11877.x. [DOI] [PubMed] [Google Scholar]
  25. Wilhelm J. M., Jessop J. J., Pettitt S. E. Aminoglycoside antibiotics and eukaryotic protein synthesis: stimulation of errors in the translation of natural messengers in extracts of cultured human cells. Biochemistry. 1978 Apr 4;17(7):1149–1153. doi: 10.1021/bi00600a002. [DOI] [PubMed] [Google Scholar]
  26. Wilson G. A., Young F. E. Intergenotic transformation of the Bacillus subtilis genospecies. J Bacteriol. 1972 Sep;111(3):705–716. doi: 10.1128/jb.111.3.705-716.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. YOUNG E. T., 2nd, SINSHEIMER R. L. A COMPARISON OF THE INITIAL ACTIONS OF SPLEEN DEOXYRIBONUCLEASE AND PANCREATIC DEOXYRIBONUCLEASE. J Biol Chem. 1965 Mar;240:1274–1280. [PubMed] [Google Scholar]
  28. Zetter B. R., Cohen P. S. Post-transcriptional regulation of T4 enzyme synthesis. Arch Biochem Biophys. 1974 Jun;162(2):560–567. doi: 10.1016/0003-9861(74)90216-1. [DOI] [PubMed] [Google Scholar]

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