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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: Biomaterials. 2010 Feb 2;31(12):3185–3200. doi: 10.1016/j.biomaterials.2010.01.041

Figure 8.

Figure 8

Biochemical analysis of cardiomyogenic differentiation of BMSCs/ECMs co-culture in 3-D collagen-gel tubular scaffold. Quantitative analysis of ANP and BNP hormones in myotube culture supernatants over a period of 14 days measured by enzyme linked immunosorbent assay, ELISA. (A) Quantification of ANP (Nppa protein) levels was compared between ECMs only cultures and BMSCs/ECMs co-cultures. *p < 0.05. The data represent the mean ± SEM of triplicate cultures (n = 3). (B) Quantification of BNP (Nppb protein) levels was compared between ECMs only cultures and BMSCs/ECMs co-cultures. *p < 0.05. The data represent the mean ± SEM of triplicate cultures (n = 3).