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. 1980 Aug;35(2):331–339. doi: 10.1128/jvi.35.2.331-339.1980

Free viral DNA in BK virus-induced hamster tumor cells.

J ter Schegget, J Voves, A van Strien, J van der Noordaa
PMCID: PMC288817  PMID: 6255174

Abstract

The biological properties of nine clonal lines of BK virus-induced hamster tumor cells were studied. All clonal lines were oncogenic and showed an enhanced ability to form colonies in semisolid medium. The cells of each clonal line contained T antigen; no virus could be rescued from any of the clonal lines. The number of viral DNA copies was determined in three of the clonal lines and varied from 10 to 20 copies per diploid amount of cell DNA. The state of the viral genome was studied in these lines, and the great majority of the viral DNA molecules appeared to be present as free (nonintegrated) molecules. At least six length classes of free defective BK virus DNA molecules, which all lacked a part of the late region of the genome, were detected in these cells. Three of the six length classes of BK virus DNA molecules acquired a TaqI recognition site, which suggested substitution of cellular DNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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