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. Author manuscript; available in PMC: 2011 May 18.
Published in final edited form as: Biochemistry. 2010 May 18;49(19):4159–4168. doi: 10.1021/bi100150v

Table 3.

Relative activity of TEG12 mutant sulfotransferases1

Catalytic
or PAP
binding		 Activity
(%)		 V3
aglycone
binding		 Activity
(%)		 Non-V3
aglycone
binding		 Activity
(%)
S9A N/A M232A 74 R101A 29
K12A 0 K233A 79 S106A 92
T16A 0 G234A 82 R107A 82
W17A N/A G235A 100 D108A 82
K65A 61 P236A 73 Y167A N/A
H67A N/A G238A 56 K171A 69
H67E N/A A239R 37 E206A 0
H67Q 0 R240A 48 R207A 79
R90A N/A P241A 0 E210A 97
S98A 27 Q242A 64 E212A 26
K213A 99
R214A 67
R248A 196
Q251A 41
1

N/A indicates a mutation that produced an insoluble protein