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. 1980 Aug;35(2):519–530. doi: 10.1128/jvi.35.2.519-530.1980

Mutations in Coliphage P1 Affecting Host Cell Lysis

Jean Tweedy Walker 1, Donald H Walker Jr 1
PMCID: PMC288837  PMID: 16789200

Abstract

A total of 103 amber mutants of coliphage P1 were tested for lysis of nonpermissive cells. Of these, 83 caused cell lysis at the normal lysis time and have defects in particle morphogenesis. Five amber mutants, with mutations in the same gene (gene 2), caused premature lysis and may have a defect in a lysis regulator. Fifteen amber mutants were unable to cause cell lysis. Artificially lysed cells infected with five of these mutants produced viable phage particles, and phage particles were seen in thin sections of unlysed, infected cells. However, phage production by these mutants was not continued after the normal lysis time. We conclude that the defect of these five mutants is in a lysis function. The five mutations were found to be in the same gene (designated gene 17). The remaining 10 amber mutants, whose mutations were found to be in the same gene (gene 10), were also unable to cause cell lysis. They differed from those in gene 17 in that no viable phage particles were produced from artificially lysed cells, and no phage particles were seen in thin sections of unlysed, infected cells. We conclude that the gene 10 mutants cannot synthesize late proteins, and it is possible that gene 10 may code for a regulator of late gene expression for P1.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BERTANI G. Studies on lysogenesis. I. The mode of phage liberation by lysogenic Escherichia coli. J Bacteriol. 1951 Sep;62(3):293–300. doi: 10.1128/jb.62.3.293-300.1951. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bächi B., Arber W. Physical mapping of BglII, BamHI, EcoRI, HindIII and PstI restriction fragments of bacteriophage P1 DNA. Mol Gen Genet. 1977 Jun 24;153(3):311–324. doi: 10.1007/BF00431596. [DOI] [PubMed] [Google Scholar]
  3. CAMPBELL A. Sensitive mutants of bacteriophage lambda. Virology. 1961 May;14:22–32. doi: 10.1016/0042-6822(61)90128-3. [DOI] [PubMed] [Google Scholar]
  4. Campbell J. H., Rolfe B. G. Evidence for a dual control of the initiation of host-cell lysis caused by phage lambda. Mol Gen Genet. 1975 Aug 5;139(1):1–8. doi: 10.1007/BF00267990. [DOI] [PubMed] [Google Scholar]
  5. Dove W. F. Action of the lambda chromosome. I. Control of functions late in bacteriophage development. J Mol Biol. 1966 Aug;19(1):187–201. doi: 10.1016/s0022-2836(66)80060-8. [DOI] [PubMed] [Google Scholar]
  6. Edgar R. S., Wood W. B. Morphogenesis of bacteriophage T4 in extracts of mutant-infected cells. Proc Natl Acad Sci U S A. 1966 Mar;55(3):498–505. doi: 10.1073/pnas.55.3.498. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Enquist L. W., Skalka A. Replication of bacteriophage lambda DNA dependent on the function of host and viral genes. I. Interaction of red, gam and rec. J Mol Biol. 1973 Apr 5;75(2):185–212. doi: 10.1016/0022-2836(73)90016-8. [DOI] [PubMed] [Google Scholar]
  8. Goldberg A. R., Howe M. New mutations in the S cistron of bacteriophage lambda affecting host cell lysis. Virology. 1969 May;38(1):200–202. doi: 10.1016/0042-6822(69)90148-2. [DOI] [PubMed] [Google Scholar]
  9. Heilmann H., Reeve J. N., Pühler A. Identification of the repressor and repressor bypass (antirepressor) polypeptides of bacteriophage P1 synthesized in infected minicells. Mol Gen Genet. 1980 Apr;178(1):149–154. doi: 10.1007/BF00267223. [DOI] [PubMed] [Google Scholar]
  10. Hertman I., Scott J. R. Recombination of phage P1 in recombination deficient hosts. Virology. 1973 Jun;53(2):468–470. doi: 10.1016/0042-6822(73)90227-4. [DOI] [PubMed] [Google Scholar]
  11. Iida S., Arber W. Plaque forming specialized transducing phage P1: isolation of P1CmSmSu, a precursor of P1Cm. Mol Gen Genet. 1977 Jun 24;153(3):259–269. doi: 10.1007/BF00431591. [DOI] [PubMed] [Google Scholar]
  12. Joyner A., Isaacs L. N., Echols H., Sly W. S. DNA replication and messenger RNA production after induction of wild-type lambda bacteriophage and lambda mutants. J Mol Biol. 1966 Aug;19(1):174–186. doi: 10.1016/s0022-2836(66)80059-1. [DOI] [PubMed] [Google Scholar]
  13. Lickfeld K. G., Menge B. Morphogenesis of bacteriophage lambda: electron microscopy of thin sections. J Mol Biol. 1976 May 15;103(2):299–318. doi: 10.1016/0022-2836(76)90314-4. [DOI] [PubMed] [Google Scholar]
  14. Lindahl G. Characterization of conditional lethal mutants of bacteriophage P2. Mol Gen Genet. 1974 Feb 6;128(3):249–260. doi: 10.1007/BF00267114. [DOI] [PubMed] [Google Scholar]
  15. Mural R. J., Chesney R. H., Vapnek D., Kropf M. M., Scott J. R. Isolation and characterization of cloned fragments of bacteriophage P1 DNA. Virology. 1979 Mar;93(2):387–397. doi: 10.1016/0042-6822(79)90243-5. [DOI] [PubMed] [Google Scholar]
  16. Oda K. I., Sakakibara Y., Tomizawa J. I. Regulation of transcription of the lambda bacteriophage genome. Virology. 1969 Dec;39(4):901–918. doi: 10.1016/0042-6822(69)90026-9. [DOI] [PubMed] [Google Scholar]
  17. Reader R. W., Siminovitch L. Lysis defective mutants of bacteriophage lambda: genetics and physiology of S cistron mutants. Virology. 1971 Mar;43(3):607–622. doi: 10.1016/0042-6822(71)90286-8. [DOI] [PubMed] [Google Scholar]
  18. Reader R. W., Siminovitch L. Lysis defective mutants of bacteriophage lambda: on the role of the S function in lysis. Virology. 1971 Mar;43(3):623–637. doi: 10.1016/0042-6822(71)90287-x. [DOI] [PubMed] [Google Scholar]
  19. Reeve J. N. Bacteriophage infection of minicells: a general method for identification of "in vivo" bacteriophage directed polypeptide biosynthesis. Mol Gen Genet. 1977 Dec 14;158(1):73–79. doi: 10.1007/BF00455121. [DOI] [PubMed] [Google Scholar]
  20. Rolfe B. G., Campbell J. H. Genetic and physiological control of host cell lysis by bacteriophage lambda. J Virol. 1977 Sep;23(3):626–636. doi: 10.1128/jvi.23.3.626-636.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Scott J. R. A new gene controlling lysogeny in phage P1. Virology. 1972 Apr;48(1):282–283. doi: 10.1016/0042-6822(72)90139-0. [DOI] [PubMed] [Google Scholar]
  22. Scott J. R. Clear plaque mutants of phage P1. Virology. 1970 May;41(1):66–71. doi: 10.1016/0042-6822(70)90054-1. [DOI] [PubMed] [Google Scholar]
  23. Scott J. R. Genetic studies on bacteriophage P1. Virology. 1968 Dec;36(4):564–574. doi: 10.1016/0042-6822(68)90188-8. [DOI] [PubMed] [Google Scholar]
  24. Scott J. R., Kropf M. M. Location of new clear plaque genes on the P1 map. Virology. 1977 Oct 15;82(2):362–368. doi: 10.1016/0042-6822(77)90011-3. [DOI] [PubMed] [Google Scholar]
  25. Sternberg N. A characterization of bacteriophage P1 DNA fragments cloned in a lambda vector. Virology. 1979 Jul 15;96(1):129–142. doi: 10.1016/0042-6822(79)90179-x. [DOI] [PubMed] [Google Scholar]
  26. Taylor A. Endopeptidase activity of phage lamba-endolysin. Nat New Biol. 1971 Dec 1;234(48):144–145. doi: 10.1038/newbio234144a0. [DOI] [PubMed] [Google Scholar]
  27. Tsugita A., Inouye M. Purification of bacteriophage T4 lysozyme. J Biol Chem. 1968 Jan 25;243(2):391–397. [PubMed] [Google Scholar]
  28. Walker D. H., Jr, Walker J. T. Genetic studies of coliphage P1. I. Mapping by use of prophage deletions. J Virol. 1975 Sep;16(3):525–534. doi: 10.1128/jvi.16.3.525-534.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Walker D. H., Jr, Walker J. T. Genetic studies of coliphage P1. III. Extended genetic map. J Virol. 1976 Oct;20(1):177–187. doi: 10.1128/jvi.20.1.177-187.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Young R., Way J., Way S., Yin J., Syvanen M. Transposition mutagenesis of bacteriophage lambda: a new gene affecting cell lysis. J Mol Biol. 1979 Aug 15;132(3):307–322. doi: 10.1016/0022-2836(79)90262-6. [DOI] [PubMed] [Google Scholar]

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