Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1980 Aug;35(2):551–554. doi: 10.1128/jvi.35.2.551-554.1980

Bacteriophage T4-related macromolecular synthesis under restriction of plasmid Rts1.

N Raghavan, M Ishaq, A Kaji
PMCID: PMC288841  PMID: 6255193

Abstract

Rts1 is a plasmid which confers upon the host bacteria the capacity to restrict T4 bacteriophage growth at 32 degrees C but not at 42 degrees C. Pulse-labeling of phage-infected cells showed that Rts1 restricts the synthesis of T1 DNA. Despite efficient restriction of T4 phage growth and DNA synthesis, infected Escherichia coli 20SO harboring Rts1 synthesized both early and late T4 phage RNA. Synthesis of early T4 phage RNA under restrictive conditions (32 degrees C) was almost equal to that found under nonrestrictive conditions, and a lesser, but significant, amount of late T4 phage RNA was made in almost complete absence of T4 DNA synthesis. Moreover, very little, if any, T4 phage-coded lysozyme was detected in the infected E. coli 20SO/Rts1 at 32 degrees C, whereas normal amounts of lysozyme were present at 42 degrees C.

Full text

PDF
551

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bautz E. K., Kasai T., Reilly E., Bautz F. A. Gene-specific mRNA. II. Regulation of mRNA synthesis in E. coli after infection with bacteriophage T4. Proc Natl Acad Sci U S A. 1966 May;55(5):1081–1088. doi: 10.1073/pnas.55.5.1081. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bolle A., Epstein R. H., Salser W., Geiduschek E. P. Transcription during bacteriophage T4 development: synthesis and relative stability of early and late RNA. J Mol Biol. 1968 Feb 14;31(3):325–348. doi: 10.1016/0022-2836(68)90413-0. [DOI] [PubMed] [Google Scholar]
  3. Boyer H. W. DNA restriction and modification mechanisms in bacteria. Annu Rev Microbiol. 1971;25:153–176. doi: 10.1146/annurev.mi.25.100171.001101. [DOI] [PubMed] [Google Scholar]
  4. Dharmalingam K., Goldberg E. B. Mechanism localisation and control of restriction cleavage of phage T4 and lambda chromosomes in vivo. Nature. 1976 Apr 1;260(5550):406–410. doi: 10.1038/260406a0. [DOI] [PubMed] [Google Scholar]
  5. Dharmalingam K., Goldberg E. B. Restriction in vivo. III. General effects of glucosylation and restriction on phage T4 gene expression and replication. Virology. 1979 Jul 30;96(2):393–403. doi: 10.1016/0042-6822(79)90097-7. [DOI] [PubMed] [Google Scholar]
  6. DiJoseph C. G., Bayer M. E., Kaji A. Host cell growth in the presence of the thermosensitive drug resistance factor, Rts1. J Bacteriol. 1973 Jul;115(1):399–410. doi: 10.1128/jb.115.1.399-410.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. DiJoseph C. G., Kaji A. Change in the cell envelope of Escherichia coli carrying the thermosensitive drug resistance factor, Rts 1, at the nonpermissive temperature. Antimicrob Agents Chemother. 1975 Oct;8(4):504–509. doi: 10.1128/aac.8.4.504. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. DiJoseph C. G. The thermosensitive lesion in the replication of the drug resistance factor, Rts1. Proc Natl Acad Sci U S A. 1974 Jun;71(6):2515–2519. doi: 10.1073/pnas.71.6.2515. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Duckworth D. H. Biological activity of bacteriophage ghosts and "take-over" of host functions by bacteriophage. Bacteriol Rev. 1970 Sep;34(3):344–363. doi: 10.1128/br.34.3.344-363.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gillespie D., Spiegelman S. A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane. J Mol Biol. 1965 Jul;12(3):829–842. doi: 10.1016/s0022-2836(65)80331-x. [DOI] [PubMed] [Google Scholar]
  11. Guha A., Szybalski W. Fractionation of the complementary strands of coliphage T4 DNA based on the asymmetric distribution of the poly U and poly U,G binding sites. Virology. 1968 Apr;34(4):608–616. doi: 10.1016/0042-6822(68)90082-2. [DOI] [PubMed] [Google Scholar]
  12. Hattman S., Revel H. R., Luria S. E. Enzyme synthesis directed by nonglucosylated T-even bacteriophages in restrictive hosts. Virology. 1966 Nov;30(3):427–438. doi: 10.1016/0042-6822(66)90120-6. [DOI] [PubMed] [Google Scholar]
  13. Ishaq M., Kaji A. Mechanism of T4 phage restriction by plasmid Rts 1. Cleavage of T4 phage DNA by Rts 1-specific enzyme. J Biol Chem. 1980 May 10;255(9):4040–4047. [PubMed] [Google Scholar]
  14. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  15. Luria S. E. Reactivation of Irradiated Bacteriophage by Transfer of Self-Reproducing Units. Proc Natl Acad Sci U S A. 1947 Sep;33(9):253–264. doi: 10.1073/pnas.33.9.253. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. O'Farrell P. Z., Gold L. M. Bacteriophage T4 gene expression. Evidence for two classes of prereplicative cistrons. J Biol Chem. 1973 Aug 10;248(15):5502–5511. [PubMed] [Google Scholar]
  17. Pizer L. I., Smith H. S., Miovic M., Pylkas L. Effect of prophage W on the propagation of bacteriophages T2 and T4. J Virol. 1968 Nov;2(11):1339–1345. doi: 10.1128/jvi.2.11.1339-1345.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Revel H. R., Luria S. E. DNA-glucosylation in T-even phage: genetic determination and role in phagehost interaction. Annu Rev Genet. 1970;4(0):177–192. doi: 10.1146/annurev.ge.04.120170.001141. [DOI] [PubMed] [Google Scholar]
  19. Riva S., Cascino A., Geiduschek E. P. Coupling of late transcription to viral replication in bacteriophage T4 development. J Mol Biol. 1970 Nov 28;54(1):85–102. doi: 10.1016/0022-2836(70)90447-x. [DOI] [PubMed] [Google Scholar]
  20. SEKIGUCHI M., COHEN S. S. THE SYNTHESIS OF MESSENGER RNA WITHOUT PROTEIN SYNTHESIS. II. SYNTHESIS OF PHAGE-INDUCED RNA AND SEQUENTIAL ENZYME PRODUCTION. J Mol Biol. 1964 May;8:638–659. doi: 10.1016/s0022-2836(64)80114-5. [DOI] [PubMed] [Google Scholar]
  21. Silverstein J. L., Goldberg E. B. T4 DNA injection. II. Protection of entering DNA from host exonuclease V. Virology. 1976 Jul 1;72(1):212–223. doi: 10.1016/0042-6822(76)90324-x. [DOI] [PubMed] [Google Scholar]
  22. Takeishi K., Kaji A. Presence of active polyribosomes in bacterial cells infected with T4 bacteriophage ghosts. J Virol. 1975 Jul;16(1):62–69. doi: 10.1128/jvi.16.1.62-69.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Wu R., Geiduschek E. P. The role of replication proteins in the regulation of bacteriophage T4 transcription. II. Gene 45 and late transcription uncoupled from replication. J Mol Biol. 1975 Aug 25;96(4):539–562. doi: 10.1016/0022-2836(75)90138-2. [DOI] [PubMed] [Google Scholar]
  24. Yokota T., Kanamaru Y., Mori R., Akiba T. Recombination between a thermosensitive kanamycin resistance factor and a nonthermosensitive multiple-drug resistant factor. J Bacteriol. 1969 Jun;98(3):863–873. doi: 10.1128/jb.98.3.863-873.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES